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COVID-19 diagnosis by RT-qPCR in alternative specimens
BACKGROUND: The high demand for adequate material for the gold standard reverse transcription real-time polymerase chain reaction (RT-qPCR)-based diagnosis imposed by the Coronavirus disease 2019 (COVID-19) pandemic, combined with the inherent contamination risks for healthcare workers during nasoph...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto Oswaldo Cruz, Ministério da Saúde
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8370469/ https://www.ncbi.nlm.nih.gov/pubmed/34406222 http://dx.doi.org/10.1590/0074-02760210085 |
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author | Gonçalves, Cássia Cristina Alves Barroso, Shana Priscila Coutinho Herlinger, Alice Laschuk Galliez, Rafael de Mello de Almeida, Tailah Bernardo Boullosa, Lidia Theodoro Nascimento, Erica Ramos dos Santos de Almeida, Jessica M da Costa, Raissa Mirella dos Santos Cunha da Paixão, Tatiana Monteiro Couceiro, José Nelson dos Santos Silva Frauches, Thiago Silva de Souza Jr, Wilson Rodrigues Costa, Andréa Ribeiro Faffe, Débora Souza Leitão, Isabela de Carvalho da Silva, Bianca Ortiz de Lira, Guilherme Sant’Anna de Almeida, Isabela Labarba Carvalho Ferreira, Orlando da Costa Castiñeiras, Terezinha Marta Pereira Pinto Mariani, Diana Tanuri, Amilcar |
author_facet | Gonçalves, Cássia Cristina Alves Barroso, Shana Priscila Coutinho Herlinger, Alice Laschuk Galliez, Rafael de Mello de Almeida, Tailah Bernardo Boullosa, Lidia Theodoro Nascimento, Erica Ramos dos Santos de Almeida, Jessica M da Costa, Raissa Mirella dos Santos Cunha da Paixão, Tatiana Monteiro Couceiro, José Nelson dos Santos Silva Frauches, Thiago Silva de Souza Jr, Wilson Rodrigues Costa, Andréa Ribeiro Faffe, Débora Souza Leitão, Isabela de Carvalho da Silva, Bianca Ortiz de Lira, Guilherme Sant’Anna de Almeida, Isabela Labarba Carvalho Ferreira, Orlando da Costa Castiñeiras, Terezinha Marta Pereira Pinto Mariani, Diana Tanuri, Amilcar |
author_sort | Gonçalves, Cássia Cristina Alves |
collection | PubMed |
description | BACKGROUND: The high demand for adequate material for the gold standard reverse transcription real-time polymerase chain reaction (RT-qPCR)-based diagnosis imposed by the Coronavirus disease 2019 (COVID-19) pandemic, combined with the inherent contamination risks for healthcare workers during nasopharyngeal swab (NP) sample collection and the discomfort it causes patients, brought the need to identify alternative specimens suitable for the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). OBJECTIVES: The aim of this work was to compare saliva and gingival fluid swabs to NP swabs as specimens for RT-qPCR-based SARS-CoV-2 diagnosis. METHODS: We compared gingival fluid swabs (n = 158) and saliva (n = 207) to the rayon-tipped NP swabs obtained from mild-symptomatic and asymptomatic subjects as specimens for RT-qPCR for SARS-CoV-2 detection. FINDINGS: When compared to NP swabs, gingival fluid swabs had a concordance rate of 15.4% among positive samples, zero among inconclusive, and 100% among negative ones. For saliva samples, the concordance rate was 67.6% among positive samples, 42.9% among inconclusive, and 96.8% among negative ones. However, the concordance rate between saliva and NP swabs was higher (96.9%) within samples with lower cycle threshold (Ct) values (Ct > 10 ≤ 25). MAIN CONCLUSIONS: Our data suggests that whereas gingival fluid swabs are not substitutes for NP swabs, saliva might be considered whenever NP swabs are not available or recommended. |
format | Online Article Text |
id | pubmed-8370469 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Instituto Oswaldo Cruz, Ministério da Saúde |
record_format | MEDLINE/PubMed |
spelling | pubmed-83704692021-08-27 COVID-19 diagnosis by RT-qPCR in alternative specimens Gonçalves, Cássia Cristina Alves Barroso, Shana Priscila Coutinho Herlinger, Alice Laschuk Galliez, Rafael de Mello de Almeida, Tailah Bernardo Boullosa, Lidia Theodoro Nascimento, Erica Ramos dos Santos de Almeida, Jessica M da Costa, Raissa Mirella dos Santos Cunha da Paixão, Tatiana Monteiro Couceiro, José Nelson dos Santos Silva Frauches, Thiago Silva de Souza Jr, Wilson Rodrigues Costa, Andréa Ribeiro Faffe, Débora Souza Leitão, Isabela de Carvalho da Silva, Bianca Ortiz de Lira, Guilherme Sant’Anna de Almeida, Isabela Labarba Carvalho Ferreira, Orlando da Costa Castiñeiras, Terezinha Marta Pereira Pinto Mariani, Diana Tanuri, Amilcar Mem Inst Oswaldo Cruz Original Article BACKGROUND: The high demand for adequate material for the gold standard reverse transcription real-time polymerase chain reaction (RT-qPCR)-based diagnosis imposed by the Coronavirus disease 2019 (COVID-19) pandemic, combined with the inherent contamination risks for healthcare workers during nasopharyngeal swab (NP) sample collection and the discomfort it causes patients, brought the need to identify alternative specimens suitable for the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). OBJECTIVES: The aim of this work was to compare saliva and gingival fluid swabs to NP swabs as specimens for RT-qPCR-based SARS-CoV-2 diagnosis. METHODS: We compared gingival fluid swabs (n = 158) and saliva (n = 207) to the rayon-tipped NP swabs obtained from mild-symptomatic and asymptomatic subjects as specimens for RT-qPCR for SARS-CoV-2 detection. FINDINGS: When compared to NP swabs, gingival fluid swabs had a concordance rate of 15.4% among positive samples, zero among inconclusive, and 100% among negative ones. For saliva samples, the concordance rate was 67.6% among positive samples, 42.9% among inconclusive, and 96.8% among negative ones. However, the concordance rate between saliva and NP swabs was higher (96.9%) within samples with lower cycle threshold (Ct) values (Ct > 10 ≤ 25). MAIN CONCLUSIONS: Our data suggests that whereas gingival fluid swabs are not substitutes for NP swabs, saliva might be considered whenever NP swabs are not available or recommended. Instituto Oswaldo Cruz, Ministério da Saúde 2021-08-13 /pmc/articles/PMC8370469/ /pubmed/34406222 http://dx.doi.org/10.1590/0074-02760210085 Text en https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License |
spellingShingle | Original Article Gonçalves, Cássia Cristina Alves Barroso, Shana Priscila Coutinho Herlinger, Alice Laschuk Galliez, Rafael de Mello de Almeida, Tailah Bernardo Boullosa, Lidia Theodoro Nascimento, Erica Ramos dos Santos de Almeida, Jessica M da Costa, Raissa Mirella dos Santos Cunha da Paixão, Tatiana Monteiro Couceiro, José Nelson dos Santos Silva Frauches, Thiago Silva de Souza Jr, Wilson Rodrigues Costa, Andréa Ribeiro Faffe, Débora Souza Leitão, Isabela de Carvalho da Silva, Bianca Ortiz de Lira, Guilherme Sant’Anna de Almeida, Isabela Labarba Carvalho Ferreira, Orlando da Costa Castiñeiras, Terezinha Marta Pereira Pinto Mariani, Diana Tanuri, Amilcar COVID-19 diagnosis by RT-qPCR in alternative specimens |
title | COVID-19 diagnosis by RT-qPCR in alternative specimens |
title_full | COVID-19 diagnosis by RT-qPCR in alternative specimens |
title_fullStr | COVID-19 diagnosis by RT-qPCR in alternative specimens |
title_full_unstemmed | COVID-19 diagnosis by RT-qPCR in alternative specimens |
title_short | COVID-19 diagnosis by RT-qPCR in alternative specimens |
title_sort | covid-19 diagnosis by rt-qpcr in alternative specimens |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8370469/ https://www.ncbi.nlm.nih.gov/pubmed/34406222 http://dx.doi.org/10.1590/0074-02760210085 |
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