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author Gonçalves, Cássia Cristina Alves
Barroso, Shana Priscila Coutinho
Herlinger, Alice Laschuk
Galliez, Rafael de Mello
de Almeida, Tailah Bernardo
Boullosa, Lidia Theodoro
Nascimento, Erica Ramos dos Santos
de Almeida, Jessica M
da Costa, Raissa Mirella dos Santos Cunha
da Paixão, Tatiana Monteiro
Couceiro, José Nelson dos Santos Silva
Frauches, Thiago Silva
de Souza Jr, Wilson Rodrigues
Costa, Andréa Ribeiro
Faffe, Débora Souza
Leitão, Isabela de Carvalho
da Silva, Bianca Ortiz
de Lira, Guilherme Sant’Anna
de Almeida, Isabela Labarba Carvalho
Ferreira, Orlando da Costa
Castiñeiras, Terezinha Marta Pereira Pinto
Mariani, Diana
Tanuri, Amilcar
author_facet Gonçalves, Cássia Cristina Alves
Barroso, Shana Priscila Coutinho
Herlinger, Alice Laschuk
Galliez, Rafael de Mello
de Almeida, Tailah Bernardo
Boullosa, Lidia Theodoro
Nascimento, Erica Ramos dos Santos
de Almeida, Jessica M
da Costa, Raissa Mirella dos Santos Cunha
da Paixão, Tatiana Monteiro
Couceiro, José Nelson dos Santos Silva
Frauches, Thiago Silva
de Souza Jr, Wilson Rodrigues
Costa, Andréa Ribeiro
Faffe, Débora Souza
Leitão, Isabela de Carvalho
da Silva, Bianca Ortiz
de Lira, Guilherme Sant’Anna
de Almeida, Isabela Labarba Carvalho
Ferreira, Orlando da Costa
Castiñeiras, Terezinha Marta Pereira Pinto
Mariani, Diana
Tanuri, Amilcar
author_sort Gonçalves, Cássia Cristina Alves
collection PubMed
description BACKGROUND: The high demand for adequate material for the gold standard reverse transcription real-time polymerase chain reaction (RT-qPCR)-based diagnosis imposed by the Coronavirus disease 2019 (COVID-19) pandemic, combined with the inherent contamination risks for healthcare workers during nasopharyngeal swab (NP) sample collection and the discomfort it causes patients, brought the need to identify alternative specimens suitable for the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). OBJECTIVES: The aim of this work was to compare saliva and gingival fluid swabs to NP swabs as specimens for RT-qPCR-based SARS-CoV-2 diagnosis. METHODS: We compared gingival fluid swabs (n = 158) and saliva (n = 207) to the rayon-tipped NP swabs obtained from mild-symptomatic and asymptomatic subjects as specimens for RT-qPCR for SARS-CoV-2 detection. FINDINGS: When compared to NP swabs, gingival fluid swabs had a concordance rate of 15.4% among positive samples, zero among inconclusive, and 100% among negative ones. For saliva samples, the concordance rate was 67.6% among positive samples, 42.9% among inconclusive, and 96.8% among negative ones. However, the concordance rate between saliva and NP swabs was higher (96.9%) within samples with lower cycle threshold (Ct) values (Ct > 10 ≤ 25). MAIN CONCLUSIONS: Our data suggests that whereas gingival fluid swabs are not substitutes for NP swabs, saliva might be considered whenever NP swabs are not available or recommended.
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spelling pubmed-83704692021-08-27 COVID-19 diagnosis by RT-qPCR in alternative specimens Gonçalves, Cássia Cristina Alves Barroso, Shana Priscila Coutinho Herlinger, Alice Laschuk Galliez, Rafael de Mello de Almeida, Tailah Bernardo Boullosa, Lidia Theodoro Nascimento, Erica Ramos dos Santos de Almeida, Jessica M da Costa, Raissa Mirella dos Santos Cunha da Paixão, Tatiana Monteiro Couceiro, José Nelson dos Santos Silva Frauches, Thiago Silva de Souza Jr, Wilson Rodrigues Costa, Andréa Ribeiro Faffe, Débora Souza Leitão, Isabela de Carvalho da Silva, Bianca Ortiz de Lira, Guilherme Sant’Anna de Almeida, Isabela Labarba Carvalho Ferreira, Orlando da Costa Castiñeiras, Terezinha Marta Pereira Pinto Mariani, Diana Tanuri, Amilcar Mem Inst Oswaldo Cruz Original Article BACKGROUND: The high demand for adequate material for the gold standard reverse transcription real-time polymerase chain reaction (RT-qPCR)-based diagnosis imposed by the Coronavirus disease 2019 (COVID-19) pandemic, combined with the inherent contamination risks for healthcare workers during nasopharyngeal swab (NP) sample collection and the discomfort it causes patients, brought the need to identify alternative specimens suitable for the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). OBJECTIVES: The aim of this work was to compare saliva and gingival fluid swabs to NP swabs as specimens for RT-qPCR-based SARS-CoV-2 diagnosis. METHODS: We compared gingival fluid swabs (n = 158) and saliva (n = 207) to the rayon-tipped NP swabs obtained from mild-symptomatic and asymptomatic subjects as specimens for RT-qPCR for SARS-CoV-2 detection. FINDINGS: When compared to NP swabs, gingival fluid swabs had a concordance rate of 15.4% among positive samples, zero among inconclusive, and 100% among negative ones. For saliva samples, the concordance rate was 67.6% among positive samples, 42.9% among inconclusive, and 96.8% among negative ones. However, the concordance rate between saliva and NP swabs was higher (96.9%) within samples with lower cycle threshold (Ct) values (Ct > 10 ≤ 25). MAIN CONCLUSIONS: Our data suggests that whereas gingival fluid swabs are not substitutes for NP swabs, saliva might be considered whenever NP swabs are not available or recommended. Instituto Oswaldo Cruz, Ministério da Saúde 2021-08-13 /pmc/articles/PMC8370469/ /pubmed/34406222 http://dx.doi.org/10.1590/0074-02760210085 Text en https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Original Article
Gonçalves, Cássia Cristina Alves
Barroso, Shana Priscila Coutinho
Herlinger, Alice Laschuk
Galliez, Rafael de Mello
de Almeida, Tailah Bernardo
Boullosa, Lidia Theodoro
Nascimento, Erica Ramos dos Santos
de Almeida, Jessica M
da Costa, Raissa Mirella dos Santos Cunha
da Paixão, Tatiana Monteiro
Couceiro, José Nelson dos Santos Silva
Frauches, Thiago Silva
de Souza Jr, Wilson Rodrigues
Costa, Andréa Ribeiro
Faffe, Débora Souza
Leitão, Isabela de Carvalho
da Silva, Bianca Ortiz
de Lira, Guilherme Sant’Anna
de Almeida, Isabela Labarba Carvalho
Ferreira, Orlando da Costa
Castiñeiras, Terezinha Marta Pereira Pinto
Mariani, Diana
Tanuri, Amilcar
COVID-19 diagnosis by RT-qPCR in alternative specimens
title COVID-19 diagnosis by RT-qPCR in alternative specimens
title_full COVID-19 diagnosis by RT-qPCR in alternative specimens
title_fullStr COVID-19 diagnosis by RT-qPCR in alternative specimens
title_full_unstemmed COVID-19 diagnosis by RT-qPCR in alternative specimens
title_short COVID-19 diagnosis by RT-qPCR in alternative specimens
title_sort covid-19 diagnosis by rt-qpcr in alternative specimens
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8370469/
https://www.ncbi.nlm.nih.gov/pubmed/34406222
http://dx.doi.org/10.1590/0074-02760210085
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