Protocol for the generation of blastocyst-like structures from mouse extended pluripotent stem cells

Extended/expanded pluripotent stem (EPS) cells can efficiently contribute to both embryonic and extraembryonic lineages in vitro and in vivo. Starting from these cells, we established a 3D differentiation system that enabled the generation of blastocyst-like structures (EPS-blastoids) through lineag...

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Detalles Bibliográficos
Autores principales: Li, Ronghui, Izpisua Belmonte, Juan Carlos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371245/
https://www.ncbi.nlm.nih.gov/pubmed/34430918
http://dx.doi.org/10.1016/j.xpro.2021.100745
Descripción
Sumario:Extended/expanded pluripotent stem (EPS) cells can efficiently contribute to both embryonic and extraembryonic lineages in vitro and in vivo. Starting from these cells, we established a 3D differentiation system that enabled the generation of blastocyst-like structures (EPS-blastoids) through lineage segregation and self-organization. We also provide proof of concept that EPS-blastoids can be generated from adult cells via cellular reprogramming. EPS-blastoids provide a unique platform for studying early embryogenesis. For complete details on the use and execution of this protocol, please refer to Li et al. (2019).

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