Protocol for the generation of blastocyst-like structures from mouse extended pluripotent stem cells

Extended/expanded pluripotent stem (EPS) cells can efficiently contribute to both embryonic and extraembryonic lineages in vitro and in vivo. Starting from these cells, we established a 3D differentiation system that enabled the generation of blastocyst-like structures (EPS-blastoids) through lineag...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Ronghui, Izpisua Belmonte, Juan Carlos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371245/
https://www.ncbi.nlm.nih.gov/pubmed/34430918
http://dx.doi.org/10.1016/j.xpro.2021.100745
_version_ 1783739601238622208
author Li, Ronghui
Izpisua Belmonte, Juan Carlos
author_facet Li, Ronghui
Izpisua Belmonte, Juan Carlos
author_sort Li, Ronghui
collection PubMed
description Extended/expanded pluripotent stem (EPS) cells can efficiently contribute to both embryonic and extraembryonic lineages in vitro and in vivo. Starting from these cells, we established a 3D differentiation system that enabled the generation of blastocyst-like structures (EPS-blastoids) through lineage segregation and self-organization. We also provide proof of concept that EPS-blastoids can be generated from adult cells via cellular reprogramming. EPS-blastoids provide a unique platform for studying early embryogenesis. For complete details on the use and execution of this protocol, please refer to Li et al. (2019).
format Online
Article
Text
id pubmed-8371245
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-83712452021-08-23 Protocol for the generation of blastocyst-like structures from mouse extended pluripotent stem cells Li, Ronghui Izpisua Belmonte, Juan Carlos STAR Protoc Protocol Extended/expanded pluripotent stem (EPS) cells can efficiently contribute to both embryonic and extraembryonic lineages in vitro and in vivo. Starting from these cells, we established a 3D differentiation system that enabled the generation of blastocyst-like structures (EPS-blastoids) through lineage segregation and self-organization. We also provide proof of concept that EPS-blastoids can be generated from adult cells via cellular reprogramming. EPS-blastoids provide a unique platform for studying early embryogenesis. For complete details on the use and execution of this protocol, please refer to Li et al. (2019). Elsevier 2021-08-13 /pmc/articles/PMC8371245/ /pubmed/34430918 http://dx.doi.org/10.1016/j.xpro.2021.100745 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Li, Ronghui
Izpisua Belmonte, Juan Carlos
Protocol for the generation of blastocyst-like structures from mouse extended pluripotent stem cells
title Protocol for the generation of blastocyst-like structures from mouse extended pluripotent stem cells
title_full Protocol for the generation of blastocyst-like structures from mouse extended pluripotent stem cells
title_fullStr Protocol for the generation of blastocyst-like structures from mouse extended pluripotent stem cells
title_full_unstemmed Protocol for the generation of blastocyst-like structures from mouse extended pluripotent stem cells
title_short Protocol for the generation of blastocyst-like structures from mouse extended pluripotent stem cells
title_sort protocol for the generation of blastocyst-like structures from mouse extended pluripotent stem cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371245/
https://www.ncbi.nlm.nih.gov/pubmed/34430918
http://dx.doi.org/10.1016/j.xpro.2021.100745
work_keys_str_mv AT lironghui protocolforthegenerationofblastocystlikestructuresfrommouseextendedpluripotentstemcells
AT izpisuabelmontejuancarlos protocolforthegenerationofblastocystlikestructuresfrommouseextendedpluripotentstemcells

Ejemplares similares