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14-3-3 Proteins Are Involved in BR-Induced Ray Petal Elongation in Gerbera hybrida

14-3-3 proteins play a major role in the regulation of primary metabolism, protein transport, ion channel activity, signal transduction and biotic/abiotic stress responses. However, their involvement in petal growth and development is largely unknown. Here, we identified and characterized the expres...

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Autores principales: Lin, Xiaohui, Huang, Shina, Huang, Gan, Chen, Yanbo, Wang, Xiaojing, Wang, Yaqin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371339/
https://www.ncbi.nlm.nih.gov/pubmed/34421972
http://dx.doi.org/10.3389/fpls.2021.718091
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author Lin, Xiaohui
Huang, Shina
Huang, Gan
Chen, Yanbo
Wang, Xiaojing
Wang, Yaqin
author_facet Lin, Xiaohui
Huang, Shina
Huang, Gan
Chen, Yanbo
Wang, Xiaojing
Wang, Yaqin
author_sort Lin, Xiaohui
collection PubMed
description 14-3-3 proteins play a major role in the regulation of primary metabolism, protein transport, ion channel activity, signal transduction and biotic/abiotic stress responses. However, their involvement in petal growth and development is largely unknown. Here, we identified and characterized the expression patterns of seven genes of the 14-3-3 family in gerbera. While none of the genes showed any tissue or developmental specificity of spatiotemporal expression, all seven predicted proteins have the nine α-helices typical of 14-3-3 proteins. Following treatment with brassinolide, an endogenous brassinosteroid, the Gh14-3-3 genes displayed various response patterns; for example, Gh14-3-3b and Gh14-3-3f reached their highest expression level at early (2 h) and late (24 h) timepoints, respectively. Further study revealed that overexpression of Gh14-3-3b or Gh14-3-3f promoted cell elongation, leading to an increase in ray petal length. By contrast, silencing of Gh14-3-3b or Gh14-3-3f inhibited petal elongation, which was eliminated partly by brassinolide. Correspondingly, the expression of petal elongation-related and brassinosteroid signaling-related genes was modified in transgenic petals. Taken together, our research suggests that Gh14-3-3b and Gh14-3-3f are positive regulators of brassinosteroid-induced ray petal elongation and thus provides novel insights into the molecular mechanism of petal growth and development.
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spelling pubmed-83713392021-08-19 14-3-3 Proteins Are Involved in BR-Induced Ray Petal Elongation in Gerbera hybrida Lin, Xiaohui Huang, Shina Huang, Gan Chen, Yanbo Wang, Xiaojing Wang, Yaqin Front Plant Sci Plant Science 14-3-3 proteins play a major role in the regulation of primary metabolism, protein transport, ion channel activity, signal transduction and biotic/abiotic stress responses. However, their involvement in petal growth and development is largely unknown. Here, we identified and characterized the expression patterns of seven genes of the 14-3-3 family in gerbera. While none of the genes showed any tissue or developmental specificity of spatiotemporal expression, all seven predicted proteins have the nine α-helices typical of 14-3-3 proteins. Following treatment with brassinolide, an endogenous brassinosteroid, the Gh14-3-3 genes displayed various response patterns; for example, Gh14-3-3b and Gh14-3-3f reached their highest expression level at early (2 h) and late (24 h) timepoints, respectively. Further study revealed that overexpression of Gh14-3-3b or Gh14-3-3f promoted cell elongation, leading to an increase in ray petal length. By contrast, silencing of Gh14-3-3b or Gh14-3-3f inhibited petal elongation, which was eliminated partly by brassinolide. Correspondingly, the expression of petal elongation-related and brassinosteroid signaling-related genes was modified in transgenic petals. Taken together, our research suggests that Gh14-3-3b and Gh14-3-3f are positive regulators of brassinosteroid-induced ray petal elongation and thus provides novel insights into the molecular mechanism of petal growth and development. Frontiers Media S.A. 2021-08-04 /pmc/articles/PMC8371339/ /pubmed/34421972 http://dx.doi.org/10.3389/fpls.2021.718091 Text en Copyright © 2021 Lin, Huang, Huang, Chen, Wang and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Lin, Xiaohui
Huang, Shina
Huang, Gan
Chen, Yanbo
Wang, Xiaojing
Wang, Yaqin
14-3-3 Proteins Are Involved in BR-Induced Ray Petal Elongation in Gerbera hybrida
title 14-3-3 Proteins Are Involved in BR-Induced Ray Petal Elongation in Gerbera hybrida
title_full 14-3-3 Proteins Are Involved in BR-Induced Ray Petal Elongation in Gerbera hybrida
title_fullStr 14-3-3 Proteins Are Involved in BR-Induced Ray Petal Elongation in Gerbera hybrida
title_full_unstemmed 14-3-3 Proteins Are Involved in BR-Induced Ray Petal Elongation in Gerbera hybrida
title_short 14-3-3 Proteins Are Involved in BR-Induced Ray Petal Elongation in Gerbera hybrida
title_sort 14-3-3 proteins are involved in br-induced ray petal elongation in gerbera hybrida
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371339/
https://www.ncbi.nlm.nih.gov/pubmed/34421972
http://dx.doi.org/10.3389/fpls.2021.718091
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