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Effect of heat acclimation on metabolic adaptations induced by endurance training in soleus rat muscle
Aerobic training leads to well‐known systemic metabolic and muscular alterations. Heat acclimation may also increase mitochondrial muscle mass. We studied the effects of heat acclimation combined with endurance training on metabolic adaptations of skeletal muscle. Thirty‐two rats were divided into f...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371354/ https://www.ncbi.nlm.nih.gov/pubmed/34405575 http://dx.doi.org/10.14814/phy2.14686 |
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author | Tardo‐Dino, Pierre‐Emmanuel Taverny, Cindy Siracusa, Julien Bourdon, Stéphanie Baugé, Stéphane Koulmann, Nathalie Malgoyre, Alexandra |
author_facet | Tardo‐Dino, Pierre‐Emmanuel Taverny, Cindy Siracusa, Julien Bourdon, Stéphanie Baugé, Stéphane Koulmann, Nathalie Malgoyre, Alexandra |
author_sort | Tardo‐Dino, Pierre‐Emmanuel |
collection | PubMed |
description | Aerobic training leads to well‐known systemic metabolic and muscular alterations. Heat acclimation may also increase mitochondrial muscle mass. We studied the effects of heat acclimation combined with endurance training on metabolic adaptations of skeletal muscle. Thirty‐two rats were divided into four groups: control (C), trained (T), heat‐acclimated (H), and trained with heat acclimation (H+T) for 6 weeks. Soleus muscle metabolism was studied, notably by the in situ measurement of mitochondrial respiration with pyruvate (Pyr) or palmitoyl‐coenzyme A (PCoA), under phosphorylating conditions ([Formula: see text]) or not ([Formula: see text]). Aerobic performance increased, and retroperitoneal fat mass decreased with training, independently of heat exposure (p < 0.001 and p < 0.001, respectively). Citrate synthase and hydroxyl‐acyl‐dehydrogenase activity increased with endurance training (p < 0.001 and p < 0.01, respectively), without any effect of heat acclimation. Training induced an increase of the [Formula: see text] and [Formula: see text] for PCoA (p < .001 and p < .01, respectively), without interference with heat acclimation. The training‐induced increase of [Formula: see text] (p < 0.01) for pyruvate oxidation was limited when combined with heat acclimation (−23%, p < 0.01). Training and heat acclimation independently increased the [Formula: see text] for pyruvate (+60% p < 0.001 and +50% p = 0.01, respectively), without an additive effect of the combination. Heat acclimation doubled the training effect on muscle glycogen storage (p < 0.001). Heat acclimation did not improve mitochondrial adaptations induced by endurance training in the soleus muscle, possibly limiting the alteration of carbohydrate oxidation while not facilitating fatty‐acid utilization. Furthermore, the increase in glycogen storage observed after HA combined with endurance training, without the improvement of pyruvate oxidation, appears to be a hypoxic metabolic phenotype. |
format | Online Article Text |
id | pubmed-8371354 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83713542021-08-23 Effect of heat acclimation on metabolic adaptations induced by endurance training in soleus rat muscle Tardo‐Dino, Pierre‐Emmanuel Taverny, Cindy Siracusa, Julien Bourdon, Stéphanie Baugé, Stéphane Koulmann, Nathalie Malgoyre, Alexandra Physiol Rep Original Articles Aerobic training leads to well‐known systemic metabolic and muscular alterations. Heat acclimation may also increase mitochondrial muscle mass. We studied the effects of heat acclimation combined with endurance training on metabolic adaptations of skeletal muscle. Thirty‐two rats were divided into four groups: control (C), trained (T), heat‐acclimated (H), and trained with heat acclimation (H+T) for 6 weeks. Soleus muscle metabolism was studied, notably by the in situ measurement of mitochondrial respiration with pyruvate (Pyr) or palmitoyl‐coenzyme A (PCoA), under phosphorylating conditions ([Formula: see text]) or not ([Formula: see text]). Aerobic performance increased, and retroperitoneal fat mass decreased with training, independently of heat exposure (p < 0.001 and p < 0.001, respectively). Citrate synthase and hydroxyl‐acyl‐dehydrogenase activity increased with endurance training (p < 0.001 and p < 0.01, respectively), without any effect of heat acclimation. Training induced an increase of the [Formula: see text] and [Formula: see text] for PCoA (p < .001 and p < .01, respectively), without interference with heat acclimation. The training‐induced increase of [Formula: see text] (p < 0.01) for pyruvate oxidation was limited when combined with heat acclimation (−23%, p < 0.01). Training and heat acclimation independently increased the [Formula: see text] for pyruvate (+60% p < 0.001 and +50% p = 0.01, respectively), without an additive effect of the combination. Heat acclimation doubled the training effect on muscle glycogen storage (p < 0.001). Heat acclimation did not improve mitochondrial adaptations induced by endurance training in the soleus muscle, possibly limiting the alteration of carbohydrate oxidation while not facilitating fatty‐acid utilization. Furthermore, the increase in glycogen storage observed after HA combined with endurance training, without the improvement of pyruvate oxidation, appears to be a hypoxic metabolic phenotype. John Wiley and Sons Inc. 2021-08-17 /pmc/articles/PMC8371354/ /pubmed/34405575 http://dx.doi.org/10.14814/phy2.14686 Text en © 2021 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Tardo‐Dino, Pierre‐Emmanuel Taverny, Cindy Siracusa, Julien Bourdon, Stéphanie Baugé, Stéphane Koulmann, Nathalie Malgoyre, Alexandra Effect of heat acclimation on metabolic adaptations induced by endurance training in soleus rat muscle |
title | Effect of heat acclimation on metabolic adaptations induced by endurance training in soleus rat muscle |
title_full | Effect of heat acclimation on metabolic adaptations induced by endurance training in soleus rat muscle |
title_fullStr | Effect of heat acclimation on metabolic adaptations induced by endurance training in soleus rat muscle |
title_full_unstemmed | Effect of heat acclimation on metabolic adaptations induced by endurance training in soleus rat muscle |
title_short | Effect of heat acclimation on metabolic adaptations induced by endurance training in soleus rat muscle |
title_sort | effect of heat acclimation on metabolic adaptations induced by endurance training in soleus rat muscle |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371354/ https://www.ncbi.nlm.nih.gov/pubmed/34405575 http://dx.doi.org/10.14814/phy2.14686 |
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