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Inflammasome Signaling Regulates the Microbial–Neuroimmune Axis and Visceral Pain in Mice
Interactions between the intestinal microbiota, immune system and nervous system are essential for homeostasis in the gut. Inflammasomes contribute to innate immunity and brain–gut interactions, but their role in microbiota–neuro–immune interactions is not clear. Therefore, we investigated the effec...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371481/ https://www.ncbi.nlm.nih.gov/pubmed/34361102 http://dx.doi.org/10.3390/ijms22158336 |
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author | Aguilera, Mònica Rossini, Valerio Hickey, Ana Simnica, Donjete Grady, Fiona Felice, Valeria D. Moloney, Amy Pawley, Lauren Fanning, Aine McCarthy, Lorraine O’Mahony, Siobhan M. Cryan, John F. Nally, Ken Shanahan, Fergus Melgar, Silvia |
author_facet | Aguilera, Mònica Rossini, Valerio Hickey, Ana Simnica, Donjete Grady, Fiona Felice, Valeria D. Moloney, Amy Pawley, Lauren Fanning, Aine McCarthy, Lorraine O’Mahony, Siobhan M. Cryan, John F. Nally, Ken Shanahan, Fergus Melgar, Silvia |
author_sort | Aguilera, Mònica |
collection | PubMed |
description | Interactions between the intestinal microbiota, immune system and nervous system are essential for homeostasis in the gut. Inflammasomes contribute to innate immunity and brain–gut interactions, but their role in microbiota–neuro–immune interactions is not clear. Therefore, we investigated the effect of the inflammasome on visceral pain and local and systemic neuroimmune responses after antibiotic-induced changes to the microbiota. Wild-type (WT) and caspase-1/11 deficient (Casp1 KO) mice were orally treated for 2 weeks with an antibiotic cocktail (Abx, Bacitracin A and Neomycin), followed by quantification of representative fecal commensals (by qPCR), cecal short chain fatty acids (by HPLC), pathways implicated in the gut–neuro-immune axis (by RT-qPCR, immunofluorescence staining, and flow cytometry) in addition to capsaicin-induced visceral pain responses. Abx-treatment in WT-mice resulted in an increase in colonic macrophages, central neuro-immune interactions, colonic inflammasome and nociceptive receptor gene expression and a reduction in capsaicin-induced visceral pain. In contrast, these responses were attenuated in Abx-treated Casp1 KO mice. Collectively, the data indicate an important role for the inflammasome pathway in functional and inflammatory gastrointestinal conditions where pain and alterations in microbiota composition are prominent. |
format | Online Article Text |
id | pubmed-8371481 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83714812021-08-19 Inflammasome Signaling Regulates the Microbial–Neuroimmune Axis and Visceral Pain in Mice Aguilera, Mònica Rossini, Valerio Hickey, Ana Simnica, Donjete Grady, Fiona Felice, Valeria D. Moloney, Amy Pawley, Lauren Fanning, Aine McCarthy, Lorraine O’Mahony, Siobhan M. Cryan, John F. Nally, Ken Shanahan, Fergus Melgar, Silvia Int J Mol Sci Article Interactions between the intestinal microbiota, immune system and nervous system are essential for homeostasis in the gut. Inflammasomes contribute to innate immunity and brain–gut interactions, but their role in microbiota–neuro–immune interactions is not clear. Therefore, we investigated the effect of the inflammasome on visceral pain and local and systemic neuroimmune responses after antibiotic-induced changes to the microbiota. Wild-type (WT) and caspase-1/11 deficient (Casp1 KO) mice were orally treated for 2 weeks with an antibiotic cocktail (Abx, Bacitracin A and Neomycin), followed by quantification of representative fecal commensals (by qPCR), cecal short chain fatty acids (by HPLC), pathways implicated in the gut–neuro-immune axis (by RT-qPCR, immunofluorescence staining, and flow cytometry) in addition to capsaicin-induced visceral pain responses. Abx-treatment in WT-mice resulted in an increase in colonic macrophages, central neuro-immune interactions, colonic inflammasome and nociceptive receptor gene expression and a reduction in capsaicin-induced visceral pain. In contrast, these responses were attenuated in Abx-treated Casp1 KO mice. Collectively, the data indicate an important role for the inflammasome pathway in functional and inflammatory gastrointestinal conditions where pain and alterations in microbiota composition are prominent. MDPI 2021-08-03 /pmc/articles/PMC8371481/ /pubmed/34361102 http://dx.doi.org/10.3390/ijms22158336 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Aguilera, Mònica Rossini, Valerio Hickey, Ana Simnica, Donjete Grady, Fiona Felice, Valeria D. Moloney, Amy Pawley, Lauren Fanning, Aine McCarthy, Lorraine O’Mahony, Siobhan M. Cryan, John F. Nally, Ken Shanahan, Fergus Melgar, Silvia Inflammasome Signaling Regulates the Microbial–Neuroimmune Axis and Visceral Pain in Mice |
title | Inflammasome Signaling Regulates the Microbial–Neuroimmune Axis and Visceral Pain in Mice |
title_full | Inflammasome Signaling Regulates the Microbial–Neuroimmune Axis and Visceral Pain in Mice |
title_fullStr | Inflammasome Signaling Regulates the Microbial–Neuroimmune Axis and Visceral Pain in Mice |
title_full_unstemmed | Inflammasome Signaling Regulates the Microbial–Neuroimmune Axis and Visceral Pain in Mice |
title_short | Inflammasome Signaling Regulates the Microbial–Neuroimmune Axis and Visceral Pain in Mice |
title_sort | inflammasome signaling regulates the microbial–neuroimmune axis and visceral pain in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371481/ https://www.ncbi.nlm.nih.gov/pubmed/34361102 http://dx.doi.org/10.3390/ijms22158336 |
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