Cargando…
Facile Assembly of Thermosensitive Liposomes for Active Targeting Imaging and Synergetic Chemo-/Magnetic Hyperthermia Therapy
Cancer stem cells (CSCs) are thought to be responsible for the recurrence of liver cancer, highlighting the urgent need for the development of effective treatment regimens. In this study, 17-allylamino-17-demethoxygeldanamycin (17-AAG) and thermosensitive magnetoliposomes (TMs) conjugated to anti-CD...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371754/ https://www.ncbi.nlm.nih.gov/pubmed/34422777 http://dx.doi.org/10.3389/fbioe.2021.691091 |
_version_ | 1783739706508312576 |
---|---|
author | An, Yanli Yang, Rui Wang, Xihui Han, Yong Jia, Gang Hu, Chunmei Zhang, Zhiyuan Liu, Dongfang Tang, Qiusha |
author_facet | An, Yanli Yang, Rui Wang, Xihui Han, Yong Jia, Gang Hu, Chunmei Zhang, Zhiyuan Liu, Dongfang Tang, Qiusha |
author_sort | An, Yanli |
collection | PubMed |
description | Cancer stem cells (CSCs) are thought to be responsible for the recurrence of liver cancer, highlighting the urgent need for the development of effective treatment regimens. In this study, 17-allylamino-17-demethoxygeldanamycin (17-AAG) and thermosensitive magnetoliposomes (TMs) conjugated to anti-CD90 (CD90@17-AAG/TMs) were developed for temperature-responsive CD90-targeted synergetic chemo-/magnetic hyperthermia therapy and simultaneous imaging in vivo. The targeting ability of CD90@DiR/TMs was studied with near-infrared (NIR) resonance imaging and magnetic resonance imaging (MRI), and the antitumor effect of CD90@17-AAG/TM-mediated magnetic thermotherapy was evaluated in vivo. After treatment, the tumors were analyzed with Western blotting, hematoxylin and eosin staining, and immunohistochemical (IHC) staining. The relative intensity of fluorescence was approximately twofold higher in the targeted group than in the non-targeted group, while the T(2) relaxation time was significantly lower in the targeted group than in the non-targeted group. The combined treatment of chemotherapy, thermotherapy, and targeting therapy exhibited the most significant antitumor effect as compared to any of the treatments alone. The anti-CD90 monoclonal antibody (mAb)-targeted delivery system, CD90@17-AAG/TMs, exhibited powerful targeting and antitumor efficacies against CD90(+) liver cancer stem cells in vivo. |
format | Online Article Text |
id | pubmed-8371754 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83717542021-08-19 Facile Assembly of Thermosensitive Liposomes for Active Targeting Imaging and Synergetic Chemo-/Magnetic Hyperthermia Therapy An, Yanli Yang, Rui Wang, Xihui Han, Yong Jia, Gang Hu, Chunmei Zhang, Zhiyuan Liu, Dongfang Tang, Qiusha Front Bioeng Biotechnol Bioengineering and Biotechnology Cancer stem cells (CSCs) are thought to be responsible for the recurrence of liver cancer, highlighting the urgent need for the development of effective treatment regimens. In this study, 17-allylamino-17-demethoxygeldanamycin (17-AAG) and thermosensitive magnetoliposomes (TMs) conjugated to anti-CD90 (CD90@17-AAG/TMs) were developed for temperature-responsive CD90-targeted synergetic chemo-/magnetic hyperthermia therapy and simultaneous imaging in vivo. The targeting ability of CD90@DiR/TMs was studied with near-infrared (NIR) resonance imaging and magnetic resonance imaging (MRI), and the antitumor effect of CD90@17-AAG/TM-mediated magnetic thermotherapy was evaluated in vivo. After treatment, the tumors were analyzed with Western blotting, hematoxylin and eosin staining, and immunohistochemical (IHC) staining. The relative intensity of fluorescence was approximately twofold higher in the targeted group than in the non-targeted group, while the T(2) relaxation time was significantly lower in the targeted group than in the non-targeted group. The combined treatment of chemotherapy, thermotherapy, and targeting therapy exhibited the most significant antitumor effect as compared to any of the treatments alone. The anti-CD90 monoclonal antibody (mAb)-targeted delivery system, CD90@17-AAG/TMs, exhibited powerful targeting and antitumor efficacies against CD90(+) liver cancer stem cells in vivo. Frontiers Media S.A. 2021-08-04 /pmc/articles/PMC8371754/ /pubmed/34422777 http://dx.doi.org/10.3389/fbioe.2021.691091 Text en Copyright © 2021 An, Yang, Wang, Han, Jia, Hu, Zhang, Liu and Tang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology An, Yanli Yang, Rui Wang, Xihui Han, Yong Jia, Gang Hu, Chunmei Zhang, Zhiyuan Liu, Dongfang Tang, Qiusha Facile Assembly of Thermosensitive Liposomes for Active Targeting Imaging and Synergetic Chemo-/Magnetic Hyperthermia Therapy |
title | Facile Assembly of Thermosensitive Liposomes for Active Targeting Imaging and Synergetic Chemo-/Magnetic Hyperthermia Therapy |
title_full | Facile Assembly of Thermosensitive Liposomes for Active Targeting Imaging and Synergetic Chemo-/Magnetic Hyperthermia Therapy |
title_fullStr | Facile Assembly of Thermosensitive Liposomes for Active Targeting Imaging and Synergetic Chemo-/Magnetic Hyperthermia Therapy |
title_full_unstemmed | Facile Assembly of Thermosensitive Liposomes for Active Targeting Imaging and Synergetic Chemo-/Magnetic Hyperthermia Therapy |
title_short | Facile Assembly of Thermosensitive Liposomes for Active Targeting Imaging and Synergetic Chemo-/Magnetic Hyperthermia Therapy |
title_sort | facile assembly of thermosensitive liposomes for active targeting imaging and synergetic chemo-/magnetic hyperthermia therapy |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371754/ https://www.ncbi.nlm.nih.gov/pubmed/34422777 http://dx.doi.org/10.3389/fbioe.2021.691091 |
work_keys_str_mv | AT anyanli facileassemblyofthermosensitiveliposomesforactivetargetingimagingandsynergeticchemomagnetichyperthermiatherapy AT yangrui facileassemblyofthermosensitiveliposomesforactivetargetingimagingandsynergeticchemomagnetichyperthermiatherapy AT wangxihui facileassemblyofthermosensitiveliposomesforactivetargetingimagingandsynergeticchemomagnetichyperthermiatherapy AT hanyong facileassemblyofthermosensitiveliposomesforactivetargetingimagingandsynergeticchemomagnetichyperthermiatherapy AT jiagang facileassemblyofthermosensitiveliposomesforactivetargetingimagingandsynergeticchemomagnetichyperthermiatherapy AT huchunmei facileassemblyofthermosensitiveliposomesforactivetargetingimagingandsynergeticchemomagnetichyperthermiatherapy AT zhangzhiyuan facileassemblyofthermosensitiveliposomesforactivetargetingimagingandsynergeticchemomagnetichyperthermiatherapy AT liudongfang facileassemblyofthermosensitiveliposomesforactivetargetingimagingandsynergeticchemomagnetichyperthermiatherapy AT tangqiusha facileassemblyofthermosensitiveliposomesforactivetargetingimagingandsynergeticchemomagnetichyperthermiatherapy |