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Direct differentiation of tonsillar biopsy-derived stem cells to the neuronal lineage
BACKGROUND: Neurological disorders are considered one of the greatest burdens to global public health and a leading cause of death. Stem cell therapies hold great promise for the cure of neurological disorders, as stem cells can serve as cell replacement, while also secreting factors to enhance endo...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371824/ https://www.ncbi.nlm.nih.gov/pubmed/34407767 http://dx.doi.org/10.1186/s11658-021-00279-4 |
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author | Arad, Michal Brown, Robert A. Khatri, Raju Taylor, Rodney J. Zalzman, Michal |
author_facet | Arad, Michal Brown, Robert A. Khatri, Raju Taylor, Rodney J. Zalzman, Michal |
author_sort | Arad, Michal |
collection | PubMed |
description | BACKGROUND: Neurological disorders are considered one of the greatest burdens to global public health and a leading cause of death. Stem cell therapies hold great promise for the cure of neurological disorders, as stem cells can serve as cell replacement, while also secreting factors to enhance endogenous tissue regeneration. Adult human multipotent stem cells (MSCs) reside on blood vessels, and therefore can be found in many tissues throughout the body, including palatine tonsils. Several studies have reported the capacity of MSCs to differentiate into, among other cell types, the neuronal lineage. However, unlike the case with embryonic stem cells, it is unclear whether MSCs can develop into mature neurons. METHODS: Human tonsillar MSCs (T-MSCs) were isolated from a small, 0.6-g sample, of tonsillar biopsies with high viability and yield as we recently reported. Then, these cells were differentiated by a rapid, multi-stage procedure, into committed, post-mitotic, neuron-like cells using defined conditions. RESULTS: Here we describe for the first time the derivation and differentiation of tonsillar biopsy-derived MSCs (T-MSCs), by a rapid, multi-step protocol, into post-mitotic, neuron-like cells using defined conditions without genetic manipulation. We characterized our T-MSC-derived neuronal cells and demonstrate their robust differentiation in vitro. CONCLUSIONS: Our procedure leads to a rapid neuronal lineage commitment and loss of stemness markers, as early as three days following neurogenic differentiation. Our studies identify biopsy-derived T-MSCs as a potential source for generating neuron-like cells which may have potential use for in vitro modeling of neurodegenerative diseases or cell replacement therapies. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s11658-021-00279-4. |
format | Online Article Text |
id | pubmed-8371824 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-83718242021-08-18 Direct differentiation of tonsillar biopsy-derived stem cells to the neuronal lineage Arad, Michal Brown, Robert A. Khatri, Raju Taylor, Rodney J. Zalzman, Michal Cell Mol Biol Lett Research BACKGROUND: Neurological disorders are considered one of the greatest burdens to global public health and a leading cause of death. Stem cell therapies hold great promise for the cure of neurological disorders, as stem cells can serve as cell replacement, while also secreting factors to enhance endogenous tissue regeneration. Adult human multipotent stem cells (MSCs) reside on blood vessels, and therefore can be found in many tissues throughout the body, including palatine tonsils. Several studies have reported the capacity of MSCs to differentiate into, among other cell types, the neuronal lineage. However, unlike the case with embryonic stem cells, it is unclear whether MSCs can develop into mature neurons. METHODS: Human tonsillar MSCs (T-MSCs) were isolated from a small, 0.6-g sample, of tonsillar biopsies with high viability and yield as we recently reported. Then, these cells were differentiated by a rapid, multi-stage procedure, into committed, post-mitotic, neuron-like cells using defined conditions. RESULTS: Here we describe for the first time the derivation and differentiation of tonsillar biopsy-derived MSCs (T-MSCs), by a rapid, multi-step protocol, into post-mitotic, neuron-like cells using defined conditions without genetic manipulation. We characterized our T-MSC-derived neuronal cells and demonstrate their robust differentiation in vitro. CONCLUSIONS: Our procedure leads to a rapid neuronal lineage commitment and loss of stemness markers, as early as three days following neurogenic differentiation. Our studies identify biopsy-derived T-MSCs as a potential source for generating neuron-like cells which may have potential use for in vitro modeling of neurodegenerative diseases or cell replacement therapies. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s11658-021-00279-4. BioMed Central 2021-08-18 /pmc/articles/PMC8371824/ /pubmed/34407767 http://dx.doi.org/10.1186/s11658-021-00279-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Arad, Michal Brown, Robert A. Khatri, Raju Taylor, Rodney J. Zalzman, Michal Direct differentiation of tonsillar biopsy-derived stem cells to the neuronal lineage |
title | Direct differentiation of tonsillar biopsy-derived stem cells to the neuronal lineage |
title_full | Direct differentiation of tonsillar biopsy-derived stem cells to the neuronal lineage |
title_fullStr | Direct differentiation of tonsillar biopsy-derived stem cells to the neuronal lineage |
title_full_unstemmed | Direct differentiation of tonsillar biopsy-derived stem cells to the neuronal lineage |
title_short | Direct differentiation of tonsillar biopsy-derived stem cells to the neuronal lineage |
title_sort | direct differentiation of tonsillar biopsy-derived stem cells to the neuronal lineage |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371824/ https://www.ncbi.nlm.nih.gov/pubmed/34407767 http://dx.doi.org/10.1186/s11658-021-00279-4 |
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