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Metabolomic Analysis Provides New Insight Into Tolerance of Huanglongbing in Citrus

There have been efforts to develop citrus cultivars that are tolerant of Huanglongbing (HLB), a catastrophic phloem-limited disease. Previous studies demonstrated that continuous plant growth with phloem regeneration is one of the major characteristics of HLB tolerance. In this study, the metabolic...

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Autores principales: Suh, Joon Hyuk, Tang, Xixuan, Zhang, Yi, Gmitter, Frederick G., Wang, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371912/
https://www.ncbi.nlm.nih.gov/pubmed/34421957
http://dx.doi.org/10.3389/fpls.2021.710598
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author Suh, Joon Hyuk
Tang, Xixuan
Zhang, Yi
Gmitter, Frederick G.
Wang, Yu
author_facet Suh, Joon Hyuk
Tang, Xixuan
Zhang, Yi
Gmitter, Frederick G.
Wang, Yu
author_sort Suh, Joon Hyuk
collection PubMed
description There have been efforts to develop citrus cultivars that are tolerant of Huanglongbing (HLB), a catastrophic phloem-limited disease. Previous studies demonstrated that continuous plant growth with phloem regeneration is one of the major characteristics of HLB tolerance. In this study, the metabolic mechanisms of HLB tolerance in citrus were elucidated using a multiple pathway-targeted metabolomic approach. Comparative analysis of healthy and infected HLB-tolerant and HLB-sensitive mandarin cultivars (Citrus reticulata) revealed differentially expressed metabolic responses among different groups. Pathway enrichment analysis indicated aspartate and glutamate metabolism, purine metabolism, and biosynthesis of plant hormones were upregulated in the tolerant group, except salicylic acid signaling. Catabolic pathways linked to energy-yielding metabolism were also upregulated in the tolerant group. These metabolisms and pathways were interconnected with each other, unveiling a pivotal metabolic network associated with HLB tolerance. In the network, auxins and cytokinins, the plant hormones responsible for plant growth and phloem regeneration, were accumulated. In addition, purine metabolites serving as energy carriers and nitrogen sources of plants were increased. Only salicylic acid-related metabolites for plant defense responses were decreased in the tolerant group. Our findings may evidence the strategy of HLB-tolerant cultivars that sustain plant growth and phloem formation rather than displaying direct plant defense to overcome the disease.
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spelling pubmed-83719122021-08-19 Metabolomic Analysis Provides New Insight Into Tolerance of Huanglongbing in Citrus Suh, Joon Hyuk Tang, Xixuan Zhang, Yi Gmitter, Frederick G. Wang, Yu Front Plant Sci Plant Science There have been efforts to develop citrus cultivars that are tolerant of Huanglongbing (HLB), a catastrophic phloem-limited disease. Previous studies demonstrated that continuous plant growth with phloem regeneration is one of the major characteristics of HLB tolerance. In this study, the metabolic mechanisms of HLB tolerance in citrus were elucidated using a multiple pathway-targeted metabolomic approach. Comparative analysis of healthy and infected HLB-tolerant and HLB-sensitive mandarin cultivars (Citrus reticulata) revealed differentially expressed metabolic responses among different groups. Pathway enrichment analysis indicated aspartate and glutamate metabolism, purine metabolism, and biosynthesis of plant hormones were upregulated in the tolerant group, except salicylic acid signaling. Catabolic pathways linked to energy-yielding metabolism were also upregulated in the tolerant group. These metabolisms and pathways were interconnected with each other, unveiling a pivotal metabolic network associated with HLB tolerance. In the network, auxins and cytokinins, the plant hormones responsible for plant growth and phloem regeneration, were accumulated. In addition, purine metabolites serving as energy carriers and nitrogen sources of plants were increased. Only salicylic acid-related metabolites for plant defense responses were decreased in the tolerant group. Our findings may evidence the strategy of HLB-tolerant cultivars that sustain plant growth and phloem formation rather than displaying direct plant defense to overcome the disease. Frontiers Media S.A. 2021-08-04 /pmc/articles/PMC8371912/ /pubmed/34421957 http://dx.doi.org/10.3389/fpls.2021.710598 Text en Copyright © 2021 Suh, Tang, Zhang, Gmitter and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Suh, Joon Hyuk
Tang, Xixuan
Zhang, Yi
Gmitter, Frederick G.
Wang, Yu
Metabolomic Analysis Provides New Insight Into Tolerance of Huanglongbing in Citrus
title Metabolomic Analysis Provides New Insight Into Tolerance of Huanglongbing in Citrus
title_full Metabolomic Analysis Provides New Insight Into Tolerance of Huanglongbing in Citrus
title_fullStr Metabolomic Analysis Provides New Insight Into Tolerance of Huanglongbing in Citrus
title_full_unstemmed Metabolomic Analysis Provides New Insight Into Tolerance of Huanglongbing in Citrus
title_short Metabolomic Analysis Provides New Insight Into Tolerance of Huanglongbing in Citrus
title_sort metabolomic analysis provides new insight into tolerance of huanglongbing in citrus
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371912/
https://www.ncbi.nlm.nih.gov/pubmed/34421957
http://dx.doi.org/10.3389/fpls.2021.710598
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