Cargando…
Metabolomic Analysis Provides New Insight Into Tolerance of Huanglongbing in Citrus
There have been efforts to develop citrus cultivars that are tolerant of Huanglongbing (HLB), a catastrophic phloem-limited disease. Previous studies demonstrated that continuous plant growth with phloem regeneration is one of the major characteristics of HLB tolerance. In this study, the metabolic...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371912/ https://www.ncbi.nlm.nih.gov/pubmed/34421957 http://dx.doi.org/10.3389/fpls.2021.710598 |
_version_ | 1783739737292406784 |
---|---|
author | Suh, Joon Hyuk Tang, Xixuan Zhang, Yi Gmitter, Frederick G. Wang, Yu |
author_facet | Suh, Joon Hyuk Tang, Xixuan Zhang, Yi Gmitter, Frederick G. Wang, Yu |
author_sort | Suh, Joon Hyuk |
collection | PubMed |
description | There have been efforts to develop citrus cultivars that are tolerant of Huanglongbing (HLB), a catastrophic phloem-limited disease. Previous studies demonstrated that continuous plant growth with phloem regeneration is one of the major characteristics of HLB tolerance. In this study, the metabolic mechanisms of HLB tolerance in citrus were elucidated using a multiple pathway-targeted metabolomic approach. Comparative analysis of healthy and infected HLB-tolerant and HLB-sensitive mandarin cultivars (Citrus reticulata) revealed differentially expressed metabolic responses among different groups. Pathway enrichment analysis indicated aspartate and glutamate metabolism, purine metabolism, and biosynthesis of plant hormones were upregulated in the tolerant group, except salicylic acid signaling. Catabolic pathways linked to energy-yielding metabolism were also upregulated in the tolerant group. These metabolisms and pathways were interconnected with each other, unveiling a pivotal metabolic network associated with HLB tolerance. In the network, auxins and cytokinins, the plant hormones responsible for plant growth and phloem regeneration, were accumulated. In addition, purine metabolites serving as energy carriers and nitrogen sources of plants were increased. Only salicylic acid-related metabolites for plant defense responses were decreased in the tolerant group. Our findings may evidence the strategy of HLB-tolerant cultivars that sustain plant growth and phloem formation rather than displaying direct plant defense to overcome the disease. |
format | Online Article Text |
id | pubmed-8371912 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83719122021-08-19 Metabolomic Analysis Provides New Insight Into Tolerance of Huanglongbing in Citrus Suh, Joon Hyuk Tang, Xixuan Zhang, Yi Gmitter, Frederick G. Wang, Yu Front Plant Sci Plant Science There have been efforts to develop citrus cultivars that are tolerant of Huanglongbing (HLB), a catastrophic phloem-limited disease. Previous studies demonstrated that continuous plant growth with phloem regeneration is one of the major characteristics of HLB tolerance. In this study, the metabolic mechanisms of HLB tolerance in citrus were elucidated using a multiple pathway-targeted metabolomic approach. Comparative analysis of healthy and infected HLB-tolerant and HLB-sensitive mandarin cultivars (Citrus reticulata) revealed differentially expressed metabolic responses among different groups. Pathway enrichment analysis indicated aspartate and glutamate metabolism, purine metabolism, and biosynthesis of plant hormones were upregulated in the tolerant group, except salicylic acid signaling. Catabolic pathways linked to energy-yielding metabolism were also upregulated in the tolerant group. These metabolisms and pathways were interconnected with each other, unveiling a pivotal metabolic network associated with HLB tolerance. In the network, auxins and cytokinins, the plant hormones responsible for plant growth and phloem regeneration, were accumulated. In addition, purine metabolites serving as energy carriers and nitrogen sources of plants were increased. Only salicylic acid-related metabolites for plant defense responses were decreased in the tolerant group. Our findings may evidence the strategy of HLB-tolerant cultivars that sustain plant growth and phloem formation rather than displaying direct plant defense to overcome the disease. Frontiers Media S.A. 2021-08-04 /pmc/articles/PMC8371912/ /pubmed/34421957 http://dx.doi.org/10.3389/fpls.2021.710598 Text en Copyright © 2021 Suh, Tang, Zhang, Gmitter and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Suh, Joon Hyuk Tang, Xixuan Zhang, Yi Gmitter, Frederick G. Wang, Yu Metabolomic Analysis Provides New Insight Into Tolerance of Huanglongbing in Citrus |
title | Metabolomic Analysis Provides New Insight Into Tolerance of Huanglongbing in Citrus |
title_full | Metabolomic Analysis Provides New Insight Into Tolerance of Huanglongbing in Citrus |
title_fullStr | Metabolomic Analysis Provides New Insight Into Tolerance of Huanglongbing in Citrus |
title_full_unstemmed | Metabolomic Analysis Provides New Insight Into Tolerance of Huanglongbing in Citrus |
title_short | Metabolomic Analysis Provides New Insight Into Tolerance of Huanglongbing in Citrus |
title_sort | metabolomic analysis provides new insight into tolerance of huanglongbing in citrus |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371912/ https://www.ncbi.nlm.nih.gov/pubmed/34421957 http://dx.doi.org/10.3389/fpls.2021.710598 |
work_keys_str_mv | AT suhjoonhyuk metabolomicanalysisprovidesnewinsightintotoleranceofhuanglongbingincitrus AT tangxixuan metabolomicanalysisprovidesnewinsightintotoleranceofhuanglongbingincitrus AT zhangyi metabolomicanalysisprovidesnewinsightintotoleranceofhuanglongbingincitrus AT gmitterfrederickg metabolomicanalysisprovidesnewinsightintotoleranceofhuanglongbingincitrus AT wangyu metabolomicanalysisprovidesnewinsightintotoleranceofhuanglongbingincitrus |