Cargando…

Cre Recombinase Driver Mice Reveal Lineage-Dependent and -Independent Expression of Brs3 in the Mouse Brain

Bombesin receptor subtype-3 (BRS3) is an orphan receptor that regulates energy homeostasis. We compared Brs3 driver mice with constitutive or inducible Cre recombinase activity. The constitutive BRS3-Cre mice show a reporter signal (Cre-dependent tdTomato) in the adult brain because of lineage traci...

Descripción completa

Detalles Bibliográficos
Autores principales: Mogul, Allison S., Hadley, Colleen K., Province, Haley S., Pauli, Jordan, Gavrilova, Oksana, Xiao, Cuiying, Palmiter, Richard D., Piñol, Ramón A., Reitman, Marc L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society for Neuroscience 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371926/
https://www.ncbi.nlm.nih.gov/pubmed/34326065
http://dx.doi.org/10.1523/ENEURO.0252-21.2021
_version_ 1783739739163066368
author Mogul, Allison S.
Hadley, Colleen K.
Province, Haley S.
Pauli, Jordan
Gavrilova, Oksana
Xiao, Cuiying
Palmiter, Richard D.
Piñol, Ramón A.
Reitman, Marc L.
author_facet Mogul, Allison S.
Hadley, Colleen K.
Province, Haley S.
Pauli, Jordan
Gavrilova, Oksana
Xiao, Cuiying
Palmiter, Richard D.
Piñol, Ramón A.
Reitman, Marc L.
author_sort Mogul, Allison S.
collection PubMed
description Bombesin receptor subtype-3 (BRS3) is an orphan receptor that regulates energy homeostasis. We compared Brs3 driver mice with constitutive or inducible Cre recombinase activity. The constitutive BRS3-Cre mice show a reporter signal (Cre-dependent tdTomato) in the adult brain because of lineage tracing in the dentate gyrus, striatal patches, and indusium griseum, in addition to sites previously identified in the inducible BRS3-Cre mice (including hypothalamic and amygdala subregions, and parabrachial nucleus). We detected Brs3 reporter expression in the dentate gyrus at day 23 but not at postnatal day 1 or 5 months of age. Hypothalamic sites expressed reporter at all three time points, and striatal patches expressed Brs3 reporter at 1 day but not 5 months. Parabrachial nucleus Brs3 neurons project to the preoptic area, hypothalamus, amygdala, and thalamus. Both Cre recombinase insertions reduced Brs3 mRNA levels and BRS3 function, causing obesity phenotypes of different severity. These results demonstrate that driver mice should be characterized phenotypically and illustrate the need for knock-in strategies with less effect on the endogenous gene.
format Online
Article
Text
id pubmed-8371926
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Society for Neuroscience
record_format MEDLINE/PubMed
spelling pubmed-83719262021-08-18 Cre Recombinase Driver Mice Reveal Lineage-Dependent and -Independent Expression of Brs3 in the Mouse Brain Mogul, Allison S. Hadley, Colleen K. Province, Haley S. Pauli, Jordan Gavrilova, Oksana Xiao, Cuiying Palmiter, Richard D. Piñol, Ramón A. Reitman, Marc L. eNeuro Research Article: New Research Bombesin receptor subtype-3 (BRS3) is an orphan receptor that regulates energy homeostasis. We compared Brs3 driver mice with constitutive or inducible Cre recombinase activity. The constitutive BRS3-Cre mice show a reporter signal (Cre-dependent tdTomato) in the adult brain because of lineage tracing in the dentate gyrus, striatal patches, and indusium griseum, in addition to sites previously identified in the inducible BRS3-Cre mice (including hypothalamic and amygdala subregions, and parabrachial nucleus). We detected Brs3 reporter expression in the dentate gyrus at day 23 but not at postnatal day 1 or 5 months of age. Hypothalamic sites expressed reporter at all three time points, and striatal patches expressed Brs3 reporter at 1 day but not 5 months. Parabrachial nucleus Brs3 neurons project to the preoptic area, hypothalamus, amygdala, and thalamus. Both Cre recombinase insertions reduced Brs3 mRNA levels and BRS3 function, causing obesity phenotypes of different severity. These results demonstrate that driver mice should be characterized phenotypically and illustrate the need for knock-in strategies with less effect on the endogenous gene. Society for Neuroscience 2021-08-16 /pmc/articles/PMC8371926/ /pubmed/34326065 http://dx.doi.org/10.1523/ENEURO.0252-21.2021 Text en Copyright © 2021 Mogul et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article: New Research
Mogul, Allison S.
Hadley, Colleen K.
Province, Haley S.
Pauli, Jordan
Gavrilova, Oksana
Xiao, Cuiying
Palmiter, Richard D.
Piñol, Ramón A.
Reitman, Marc L.
Cre Recombinase Driver Mice Reveal Lineage-Dependent and -Independent Expression of Brs3 in the Mouse Brain
title Cre Recombinase Driver Mice Reveal Lineage-Dependent and -Independent Expression of Brs3 in the Mouse Brain
title_full Cre Recombinase Driver Mice Reveal Lineage-Dependent and -Independent Expression of Brs3 in the Mouse Brain
title_fullStr Cre Recombinase Driver Mice Reveal Lineage-Dependent and -Independent Expression of Brs3 in the Mouse Brain
title_full_unstemmed Cre Recombinase Driver Mice Reveal Lineage-Dependent and -Independent Expression of Brs3 in the Mouse Brain
title_short Cre Recombinase Driver Mice Reveal Lineage-Dependent and -Independent Expression of Brs3 in the Mouse Brain
title_sort cre recombinase driver mice reveal lineage-dependent and -independent expression of brs3 in the mouse brain
topic Research Article: New Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371926/
https://www.ncbi.nlm.nih.gov/pubmed/34326065
http://dx.doi.org/10.1523/ENEURO.0252-21.2021
work_keys_str_mv AT mogulallisons crerecombinasedrivermicereveallineagedependentandindependentexpressionofbrs3inthemousebrain
AT hadleycolleenk crerecombinasedrivermicereveallineagedependentandindependentexpressionofbrs3inthemousebrain
AT provincehaleys crerecombinasedrivermicereveallineagedependentandindependentexpressionofbrs3inthemousebrain
AT paulijordan crerecombinasedrivermicereveallineagedependentandindependentexpressionofbrs3inthemousebrain
AT gavrilovaoksana crerecombinasedrivermicereveallineagedependentandindependentexpressionofbrs3inthemousebrain
AT xiaocuiying crerecombinasedrivermicereveallineagedependentandindependentexpressionofbrs3inthemousebrain
AT palmiterrichardd crerecombinasedrivermicereveallineagedependentandindependentexpressionofbrs3inthemousebrain
AT pinolramona crerecombinasedrivermicereveallineagedependentandindependentexpressionofbrs3inthemousebrain
AT reitmanmarcl crerecombinasedrivermicereveallineagedependentandindependentexpressionofbrs3inthemousebrain