Cargando…
Overview of Portable Assays for the Detection of Mycotoxins, Allergens, and Sanitation Monitoring
BACKGROUND: Many food recalls are related to the presence of undeclared allergens and microorganisms in food products. To reduce these occurrences, portable diagnostic assay kits are available to quantitate mycotoxins, to detect allergens and gluten in foods and on environmental surfaces, and for sa...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8372037/ https://www.ncbi.nlm.nih.gov/pubmed/33249442 http://dx.doi.org/10.1093/jaoacint/qsaa113 |
_version_ | 1783739753716252672 |
---|---|
author | Sarver, Ronald W Almy, David J Bergeron, Eric R Strong, Benjamin F Steiner, Brent A Donofrio, Robert Lupo, Anthony J Gray, R Lucas Sperry, Amanda K |
author_facet | Sarver, Ronald W Almy, David J Bergeron, Eric R Strong, Benjamin F Steiner, Brent A Donofrio, Robert Lupo, Anthony J Gray, R Lucas Sperry, Amanda K |
author_sort | Sarver, Ronald W |
collection | PubMed |
description | BACKGROUND: Many food recalls are related to the presence of undeclared allergens and microorganisms in food products. To reduce these occurrences, portable diagnostic assay kits are available to quantitate mycotoxins, to detect allergens and gluten in foods and on environmental surfaces, and for sanitation monitoring. OBJECTIVE: This article reviews diagnostic kits that can detect sources of contamination in food and ingredients as well as on surfaces and clean-in-place rinses. METHOD: Mycotoxins and gluten were detected using lateral flow diagnostic assays. Sanitation monitoring of surfaces was completed using a chemiluminescent assay to detect adenosine 5′-triphosphate disodium salt hydrate (ATP) and another assay to detect protein. RESULTS: Gluten was detected at 10 ppm in spiked commodities and on wet and dry surfaces at 2.5 µg/100cm(2). Deoxynivalenol was quantitated in dry distillers grains plus solubles and mean results were within two SDs of those determined by HPLC. The chemiluminescent assay had an LOD of 6 fmol of ATP and was able to detect a 1:10 000 dilution of orange juice from surfaces. The protein assay detected 5 µg of bovine serum albumin (BSA) directly applied to the sampler, 100 µg of BSA on surfaces, and detected 1:10 dilutions of Greek yogurt and raw beef from surfaces. CONCLUSIONS: Portable diagnostic kits evaluated in this work provided accurate, rapid, and sensitive results for detection of mycotoxins, gluten, proteins, and ATP. These methods can be used in facilities with minimal training and provide results that are important to ensure food safety. HIGHLIGHTS: Portable methods to detect gluten, mycotoxins, proteins, and ATP are presented. |
format | Online Article Text |
id | pubmed-8372037 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-83720372021-12-28 Overview of Portable Assays for the Detection of Mycotoxins, Allergens, and Sanitation Monitoring Sarver, Ronald W Almy, David J Bergeron, Eric R Strong, Benjamin F Steiner, Brent A Donofrio, Robert Lupo, Anthony J Gray, R Lucas Sperry, Amanda K J AOAC Int Special Guest Editor (Popping & Diaz-Amigo) BACKGROUND: Many food recalls are related to the presence of undeclared allergens and microorganisms in food products. To reduce these occurrences, portable diagnostic assay kits are available to quantitate mycotoxins, to detect allergens and gluten in foods and on environmental surfaces, and for sanitation monitoring. OBJECTIVE: This article reviews diagnostic kits that can detect sources of contamination in food and ingredients as well as on surfaces and clean-in-place rinses. METHOD: Mycotoxins and gluten were detected using lateral flow diagnostic assays. Sanitation monitoring of surfaces was completed using a chemiluminescent assay to detect adenosine 5′-triphosphate disodium salt hydrate (ATP) and another assay to detect protein. RESULTS: Gluten was detected at 10 ppm in spiked commodities and on wet and dry surfaces at 2.5 µg/100cm(2). Deoxynivalenol was quantitated in dry distillers grains plus solubles and mean results were within two SDs of those determined by HPLC. The chemiluminescent assay had an LOD of 6 fmol of ATP and was able to detect a 1:10 000 dilution of orange juice from surfaces. The protein assay detected 5 µg of bovine serum albumin (BSA) directly applied to the sampler, 100 µg of BSA on surfaces, and detected 1:10 dilutions of Greek yogurt and raw beef from surfaces. CONCLUSIONS: Portable diagnostic kits evaluated in this work provided accurate, rapid, and sensitive results for detection of mycotoxins, gluten, proteins, and ATP. These methods can be used in facilities with minimal training and provide results that are important to ensure food safety. HIGHLIGHTS: Portable methods to detect gluten, mycotoxins, proteins, and ATP are presented. Oxford University Press 2020-08-25 /pmc/articles/PMC8372037/ /pubmed/33249442 http://dx.doi.org/10.1093/jaoacint/qsaa113 Text en © AOAC INTERNATIONAL 2020. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Special Guest Editor (Popping & Diaz-Amigo) Sarver, Ronald W Almy, David J Bergeron, Eric R Strong, Benjamin F Steiner, Brent A Donofrio, Robert Lupo, Anthony J Gray, R Lucas Sperry, Amanda K Overview of Portable Assays for the Detection of Mycotoxins, Allergens, and Sanitation Monitoring |
title | Overview of Portable Assays for the Detection of Mycotoxins, Allergens, and Sanitation Monitoring |
title_full | Overview of Portable Assays for the Detection of Mycotoxins, Allergens, and Sanitation Monitoring |
title_fullStr | Overview of Portable Assays for the Detection of Mycotoxins, Allergens, and Sanitation Monitoring |
title_full_unstemmed | Overview of Portable Assays for the Detection of Mycotoxins, Allergens, and Sanitation Monitoring |
title_short | Overview of Portable Assays for the Detection of Mycotoxins, Allergens, and Sanitation Monitoring |
title_sort | overview of portable assays for the detection of mycotoxins, allergens, and sanitation monitoring |
topic | Special Guest Editor (Popping & Diaz-Amigo) |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8372037/ https://www.ncbi.nlm.nih.gov/pubmed/33249442 http://dx.doi.org/10.1093/jaoacint/qsaa113 |
work_keys_str_mv | AT sarverronaldw overviewofportableassaysforthedetectionofmycotoxinsallergensandsanitationmonitoring AT almydavidj overviewofportableassaysforthedetectionofmycotoxinsallergensandsanitationmonitoring AT bergeronericr overviewofportableassaysforthedetectionofmycotoxinsallergensandsanitationmonitoring AT strongbenjaminf overviewofportableassaysforthedetectionofmycotoxinsallergensandsanitationmonitoring AT steinerbrenta overviewofportableassaysforthedetectionofmycotoxinsallergensandsanitationmonitoring AT donofriorobert overviewofportableassaysforthedetectionofmycotoxinsallergensandsanitationmonitoring AT lupoanthonyj overviewofportableassaysforthedetectionofmycotoxinsallergensandsanitationmonitoring AT grayrlucas overviewofportableassaysforthedetectionofmycotoxinsallergensandsanitationmonitoring AT sperryamandak overviewofportableassaysforthedetectionofmycotoxinsallergensandsanitationmonitoring |