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Blood group genotyping in alloimmunized multi‐transfused thalassemia patients from Iran
OBJECTIVES: Serological methods may not be reliable for RBC antigen typing, especially in multi‐transfused patients. The blood group systems provoking the most severe transfusion reactions are mainly Rh, Kell, Kidd, and Duffy. We intended to determine the genotype of these blood group system antigen...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8372074/ https://www.ncbi.nlm.nih.gov/pubmed/33963817 http://dx.doi.org/10.1002/mgg3.1701 |
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author | Sarihi, Reyhaneh Oodi, Arezoo Dadkhah Tehrani, Raziyeh Jalali, Seyedeh Farzaneh Mardani, Fahimeh Azarkeivan, Azita Gudarzi, Samira Amirizadeh, Naser |
author_facet | Sarihi, Reyhaneh Oodi, Arezoo Dadkhah Tehrani, Raziyeh Jalali, Seyedeh Farzaneh Mardani, Fahimeh Azarkeivan, Azita Gudarzi, Samira Amirizadeh, Naser |
author_sort | Sarihi, Reyhaneh |
collection | PubMed |
description | OBJECTIVES: Serological methods may not be reliable for RBC antigen typing, especially in multi‐transfused patients. The blood group systems provoking the most severe transfusion reactions are mainly Rh, Kell, Kidd, and Duffy. We intended to determine the genotype of these blood group system antigens among Iranian alloimmunized thalassemia patients using molecular methods and compare the results with serological phenotyping. METHODS: Two hundred patients participated in this study. Blood group phenotype and genotype were determined using the serological method and PCR‐SSP, respectively. The genotypes of patients with incompatibility between phenotype and genotype were re‐evaluated by RFLP‐PCR and confirmed by DNA sequencing. RESULTS: Discrepancies between phenotype and genotype results were found in 132 alleles and 83 (41.5%) patients; however, there was complete accordance between the three genotyping methods. Most discrepancies were detected in Rh and Duffy systems with 47 and 45 cases, respectively, and the main discrepancy was in the FY*B/FY*B allele when serologically showed Fy(a+b+). All 39 undetermined phenotypes, due to mixed‐field reactions, were resolved by molecular genotyping. CONCLUSION: Molecular genotyping is more reliable compared with the serological method, especially in multi‐transfused patients. Therefore, the addition of blood group genotyping to serological assays can lead to an antigen‐matched transfusion in these patients. |
format | Online Article Text |
id | pubmed-8372074 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83720742021-08-23 Blood group genotyping in alloimmunized multi‐transfused thalassemia patients from Iran Sarihi, Reyhaneh Oodi, Arezoo Dadkhah Tehrani, Raziyeh Jalali, Seyedeh Farzaneh Mardani, Fahimeh Azarkeivan, Azita Gudarzi, Samira Amirizadeh, Naser Mol Genet Genomic Med Original Articles OBJECTIVES: Serological methods may not be reliable for RBC antigen typing, especially in multi‐transfused patients. The blood group systems provoking the most severe transfusion reactions are mainly Rh, Kell, Kidd, and Duffy. We intended to determine the genotype of these blood group system antigens among Iranian alloimmunized thalassemia patients using molecular methods and compare the results with serological phenotyping. METHODS: Two hundred patients participated in this study. Blood group phenotype and genotype were determined using the serological method and PCR‐SSP, respectively. The genotypes of patients with incompatibility between phenotype and genotype were re‐evaluated by RFLP‐PCR and confirmed by DNA sequencing. RESULTS: Discrepancies between phenotype and genotype results were found in 132 alleles and 83 (41.5%) patients; however, there was complete accordance between the three genotyping methods. Most discrepancies were detected in Rh and Duffy systems with 47 and 45 cases, respectively, and the main discrepancy was in the FY*B/FY*B allele when serologically showed Fy(a+b+). All 39 undetermined phenotypes, due to mixed‐field reactions, were resolved by molecular genotyping. CONCLUSION: Molecular genotyping is more reliable compared with the serological method, especially in multi‐transfused patients. Therefore, the addition of blood group genotyping to serological assays can lead to an antigen‐matched transfusion in these patients. John Wiley and Sons Inc. 2021-05-08 /pmc/articles/PMC8372074/ /pubmed/33963817 http://dx.doi.org/10.1002/mgg3.1701 Text en © 2021 The Authors. Molecular Genetics & Genomic Medicine published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Sarihi, Reyhaneh Oodi, Arezoo Dadkhah Tehrani, Raziyeh Jalali, Seyedeh Farzaneh Mardani, Fahimeh Azarkeivan, Azita Gudarzi, Samira Amirizadeh, Naser Blood group genotyping in alloimmunized multi‐transfused thalassemia patients from Iran |
title | Blood group genotyping in alloimmunized multi‐transfused thalassemia patients from Iran |
title_full | Blood group genotyping in alloimmunized multi‐transfused thalassemia patients from Iran |
title_fullStr | Blood group genotyping in alloimmunized multi‐transfused thalassemia patients from Iran |
title_full_unstemmed | Blood group genotyping in alloimmunized multi‐transfused thalassemia patients from Iran |
title_short | Blood group genotyping in alloimmunized multi‐transfused thalassemia patients from Iran |
title_sort | blood group genotyping in alloimmunized multi‐transfused thalassemia patients from iran |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8372074/ https://www.ncbi.nlm.nih.gov/pubmed/33963817 http://dx.doi.org/10.1002/mgg3.1701 |
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