Cargando…

The Analysis of Docosahexaenoic Acid (DHA) in Dried Dog Food Enriched with an Aurantiochytrium limacinum Biomass: Matrix Extension Validation and Verification of AOAC Method 996.06

BACKGROUND: Docosahexaenoic acid (DHA) plays an important role in brain and retinal development in dogs. However, supranutritional dietary supplementation can result in health issues, including gastrointestinal bleeding, making the accurate analysis of DHA in dog food important for nutritional and w...

Descripción completa

Detalles Bibliográficos
Autores principales: Dillon, Gerald Patrick, Cardinall, Cathy, Keegan, Jason D, Yiannikouris, Alexandros, Brandl, Walter, Moran, Colm Anthony
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8372133/
https://www.ncbi.nlm.nih.gov/pubmed/33150938
http://dx.doi.org/10.1093/jaoacint/qsaa097
_version_ 1783739764137000960
author Dillon, Gerald Patrick
Cardinall, Cathy
Keegan, Jason D
Yiannikouris, Alexandros
Brandl, Walter
Moran, Colm Anthony
author_facet Dillon, Gerald Patrick
Cardinall, Cathy
Keegan, Jason D
Yiannikouris, Alexandros
Brandl, Walter
Moran, Colm Anthony
author_sort Dillon, Gerald Patrick
collection PubMed
description BACKGROUND: Docosahexaenoic acid (DHA) plays an important role in brain and retinal development in dogs. However, supranutritional dietary supplementation can result in health issues, including gastrointestinal bleeding, making the accurate analysis of DHA in dog food important for nutritional and welfare regulatory compliance. OBJECTIVE: The aim of this study was to conduct a validation and verification of the AOAC 996.06 method, and hence establish its fitness for purpose, for the analysis of DHA in dried dog food supplemented with a heterotrophically grown unextracted DHA-rich Aurantiochytrium limacinum biomass. METHODS: The AOAC 996.06 method, which involves the use of gas chromatography coupled to flame ionization detection (GC-FID), was used to conduct a validation of the analysis of DHA in dried dog food and the results were verified in a second laboratory. RESULTS: The method was found to be linear over the ranges analyzed and results were found to be within the acceptance criteria for precision and accuracy, verifying the applicability for this matrix. The selectivity and sensitivity of the method were also determined. CONCLUSIONS: The AOAC 996.06 method is fit for purpose for the analysis of DHA in dry dog food kibble. HIGHLIGHTS: The method can be applied to various dog food samples, supplemented with an unextracted Aurantiochytrium limacinum biomass, using alternative manufacturing methods, i.e. pelleted and extruded with no significant matrix effects being observed.
format Online
Article
Text
id pubmed-8372133
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-83721332021-12-28 The Analysis of Docosahexaenoic Acid (DHA) in Dried Dog Food Enriched with an Aurantiochytrium limacinum Biomass: Matrix Extension Validation and Verification of AOAC Method 996.06 Dillon, Gerald Patrick Cardinall, Cathy Keegan, Jason D Yiannikouris, Alexandros Brandl, Walter Moran, Colm Anthony J AOAC Int Animal Food, Pet Food, and Plant Nutrient Methods BACKGROUND: Docosahexaenoic acid (DHA) plays an important role in brain and retinal development in dogs. However, supranutritional dietary supplementation can result in health issues, including gastrointestinal bleeding, making the accurate analysis of DHA in dog food important for nutritional and welfare regulatory compliance. OBJECTIVE: The aim of this study was to conduct a validation and verification of the AOAC 996.06 method, and hence establish its fitness for purpose, for the analysis of DHA in dried dog food supplemented with a heterotrophically grown unextracted DHA-rich Aurantiochytrium limacinum biomass. METHODS: The AOAC 996.06 method, which involves the use of gas chromatography coupled to flame ionization detection (GC-FID), was used to conduct a validation of the analysis of DHA in dried dog food and the results were verified in a second laboratory. RESULTS: The method was found to be linear over the ranges analyzed and results were found to be within the acceptance criteria for precision and accuracy, verifying the applicability for this matrix. The selectivity and sensitivity of the method were also determined. CONCLUSIONS: The AOAC 996.06 method is fit for purpose for the analysis of DHA in dry dog food kibble. HIGHLIGHTS: The method can be applied to various dog food samples, supplemented with an unextracted Aurantiochytrium limacinum biomass, using alternative manufacturing methods, i.e. pelleted and extruded with no significant matrix effects being observed. Oxford University Press 2020-07-19 /pmc/articles/PMC8372133/ /pubmed/33150938 http://dx.doi.org/10.1093/jaoacint/qsaa097 Text en © AOAC INTERNATIONAL 2020. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Animal Food, Pet Food, and Plant Nutrient Methods
Dillon, Gerald Patrick
Cardinall, Cathy
Keegan, Jason D
Yiannikouris, Alexandros
Brandl, Walter
Moran, Colm Anthony
The Analysis of Docosahexaenoic Acid (DHA) in Dried Dog Food Enriched with an Aurantiochytrium limacinum Biomass: Matrix Extension Validation and Verification of AOAC Method 996.06
title The Analysis of Docosahexaenoic Acid (DHA) in Dried Dog Food Enriched with an Aurantiochytrium limacinum Biomass: Matrix Extension Validation and Verification of AOAC Method 996.06
title_full The Analysis of Docosahexaenoic Acid (DHA) in Dried Dog Food Enriched with an Aurantiochytrium limacinum Biomass: Matrix Extension Validation and Verification of AOAC Method 996.06
title_fullStr The Analysis of Docosahexaenoic Acid (DHA) in Dried Dog Food Enriched with an Aurantiochytrium limacinum Biomass: Matrix Extension Validation and Verification of AOAC Method 996.06
title_full_unstemmed The Analysis of Docosahexaenoic Acid (DHA) in Dried Dog Food Enriched with an Aurantiochytrium limacinum Biomass: Matrix Extension Validation and Verification of AOAC Method 996.06
title_short The Analysis of Docosahexaenoic Acid (DHA) in Dried Dog Food Enriched with an Aurantiochytrium limacinum Biomass: Matrix Extension Validation and Verification of AOAC Method 996.06
title_sort analysis of docosahexaenoic acid (dha) in dried dog food enriched with an aurantiochytrium limacinum biomass: matrix extension validation and verification of aoac method 996.06
topic Animal Food, Pet Food, and Plant Nutrient Methods
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8372133/
https://www.ncbi.nlm.nih.gov/pubmed/33150938
http://dx.doi.org/10.1093/jaoacint/qsaa097
work_keys_str_mv AT dillongeraldpatrick theanalysisofdocosahexaenoicaciddhaindrieddogfoodenrichedwithanaurantiochytriumlimacinumbiomassmatrixextensionvalidationandverificationofaoacmethod99606
AT cardinallcathy theanalysisofdocosahexaenoicaciddhaindrieddogfoodenrichedwithanaurantiochytriumlimacinumbiomassmatrixextensionvalidationandverificationofaoacmethod99606
AT keeganjasond theanalysisofdocosahexaenoicaciddhaindrieddogfoodenrichedwithanaurantiochytriumlimacinumbiomassmatrixextensionvalidationandverificationofaoacmethod99606
AT yiannikourisalexandros theanalysisofdocosahexaenoicaciddhaindrieddogfoodenrichedwithanaurantiochytriumlimacinumbiomassmatrixextensionvalidationandverificationofaoacmethod99606
AT brandlwalter theanalysisofdocosahexaenoicaciddhaindrieddogfoodenrichedwithanaurantiochytriumlimacinumbiomassmatrixextensionvalidationandverificationofaoacmethod99606
AT morancolmanthony theanalysisofdocosahexaenoicaciddhaindrieddogfoodenrichedwithanaurantiochytriumlimacinumbiomassmatrixextensionvalidationandverificationofaoacmethod99606
AT dillongeraldpatrick analysisofdocosahexaenoicaciddhaindrieddogfoodenrichedwithanaurantiochytriumlimacinumbiomassmatrixextensionvalidationandverificationofaoacmethod99606
AT cardinallcathy analysisofdocosahexaenoicaciddhaindrieddogfoodenrichedwithanaurantiochytriumlimacinumbiomassmatrixextensionvalidationandverificationofaoacmethod99606
AT keeganjasond analysisofdocosahexaenoicaciddhaindrieddogfoodenrichedwithanaurantiochytriumlimacinumbiomassmatrixextensionvalidationandverificationofaoacmethod99606
AT yiannikourisalexandros analysisofdocosahexaenoicaciddhaindrieddogfoodenrichedwithanaurantiochytriumlimacinumbiomassmatrixextensionvalidationandverificationofaoacmethod99606
AT brandlwalter analysisofdocosahexaenoicaciddhaindrieddogfoodenrichedwithanaurantiochytriumlimacinumbiomassmatrixextensionvalidationandverificationofaoacmethod99606
AT morancolmanthony analysisofdocosahexaenoicaciddhaindrieddogfoodenrichedwithanaurantiochytriumlimacinumbiomassmatrixextensionvalidationandverificationofaoacmethod99606