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Sensitivity of SARS-CoV-2 antibody tests with late convalescent sera
SARS-CoV-2-specific IgM antibodies wane during the first three months after infection and IgG antibody levels decline. This may limit the ability of antibody tests to identify previous SARS-CoV-2 infection at later time points. To examine if the diagnostic sensitivity of antibody tests falls off, we...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Author(s). Published by Elsevier Ltd.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8372433/ https://www.ncbi.nlm.nih.gov/pubmed/35262021 http://dx.doi.org/10.1016/j.jcvp.2021.100038 |
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author | Kannenberg, Judith Schnurra, Carolin Reiners, Nina Henschler, Reinhard Buhmann, Raymund Kaiser, Thorsten Biemann, Ronald Hönemann, Mario Ackermann, Grit Trawinski, Henning Jassoy, Christian |
author_facet | Kannenberg, Judith Schnurra, Carolin Reiners, Nina Henschler, Reinhard Buhmann, Raymund Kaiser, Thorsten Biemann, Ronald Hönemann, Mario Ackermann, Grit Trawinski, Henning Jassoy, Christian |
author_sort | Kannenberg, Judith |
collection | PubMed |
description | SARS-CoV-2-specific IgM antibodies wane during the first three months after infection and IgG antibody levels decline. This may limit the ability of antibody tests to identify previous SARS-CoV-2 infection at later time points. To examine if the diagnostic sensitivity of antibody tests falls off, we compared the sensitivity of two nucleoprotein-based antibody tests, the Roche Elecsis II Anti-SARS-CoV-2 and the Abbott SARS-CoV-2 IgG assay and three glycoprotein-based tests, the Abbott SARS-CoV-2 IgG II Quant, Siemens Atellica IM COV2T and Euroimmun SARS-CoV-2 assay with 53 sera obtained 6 months after SARS-CoV-2 infection. The sensitivity of the Roche, Abbott SARS-CoV-2 IgG II Quant and Siemens antibody assays was 94.3% (95% confidence interval (CI) 84.3–98.8%), 98.1 % (95% CI: 89.9–100%) and 100 % (95% CI: 93.3–100%). The sensitivity of the N-based Abbott SARS-CoV-2 IgG and the glycoprotein-based Euroimmun ELISA was 45.3 % (95% CI: 31.6–59.6%) and 83.3% (95% CI: 70.2–91.9%). The nucleoprotein-based Roche and the glycoprotein-based Abbott receptor binding domain (RBD) and Siemens tests were more sensitive than the N-based Abbott and the Euroimmun antibody tests (p = 0.0001 to p = 0.039). The N-based Abbott antibody test was less sensitive 6 months than 4–10 weeks after SARS-CoV-2 infection (p = 0.0001). The findings show that most SARS-CoV-2 antibody assays correctly identified previous infection 6 months after infection. The sensitivity of pan-Ig antibody tests was not reduced at 6 months when IgM antibodies have usually disappeared. However, one of the nucleoprotein-based antibody tests significantly lost diagnostic sensitivity over time. |
format | Online Article Text |
id | pubmed-8372433 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Author(s). Published by Elsevier Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83724332021-08-18 Sensitivity of SARS-CoV-2 antibody tests with late convalescent sera Kannenberg, Judith Schnurra, Carolin Reiners, Nina Henschler, Reinhard Buhmann, Raymund Kaiser, Thorsten Biemann, Ronald Hönemann, Mario Ackermann, Grit Trawinski, Henning Jassoy, Christian Journal of Clinical Virology Plus Article SARS-CoV-2-specific IgM antibodies wane during the first three months after infection and IgG antibody levels decline. This may limit the ability of antibody tests to identify previous SARS-CoV-2 infection at later time points. To examine if the diagnostic sensitivity of antibody tests falls off, we compared the sensitivity of two nucleoprotein-based antibody tests, the Roche Elecsis II Anti-SARS-CoV-2 and the Abbott SARS-CoV-2 IgG assay and three glycoprotein-based tests, the Abbott SARS-CoV-2 IgG II Quant, Siemens Atellica IM COV2T and Euroimmun SARS-CoV-2 assay with 53 sera obtained 6 months after SARS-CoV-2 infection. The sensitivity of the Roche, Abbott SARS-CoV-2 IgG II Quant and Siemens antibody assays was 94.3% (95% confidence interval (CI) 84.3–98.8%), 98.1 % (95% CI: 89.9–100%) and 100 % (95% CI: 93.3–100%). The sensitivity of the N-based Abbott SARS-CoV-2 IgG and the glycoprotein-based Euroimmun ELISA was 45.3 % (95% CI: 31.6–59.6%) and 83.3% (95% CI: 70.2–91.9%). The nucleoprotein-based Roche and the glycoprotein-based Abbott receptor binding domain (RBD) and Siemens tests were more sensitive than the N-based Abbott and the Euroimmun antibody tests (p = 0.0001 to p = 0.039). The N-based Abbott antibody test was less sensitive 6 months than 4–10 weeks after SARS-CoV-2 infection (p = 0.0001). The findings show that most SARS-CoV-2 antibody assays correctly identified previous infection 6 months after infection. The sensitivity of pan-Ig antibody tests was not reduced at 6 months when IgM antibodies have usually disappeared. However, one of the nucleoprotein-based antibody tests significantly lost diagnostic sensitivity over time. The Author(s). Published by Elsevier Ltd. 2021-09 2021-08-18 /pmc/articles/PMC8372433/ /pubmed/35262021 http://dx.doi.org/10.1016/j.jcvp.2021.100038 Text en © 2021 The Author(s) Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Kannenberg, Judith Schnurra, Carolin Reiners, Nina Henschler, Reinhard Buhmann, Raymund Kaiser, Thorsten Biemann, Ronald Hönemann, Mario Ackermann, Grit Trawinski, Henning Jassoy, Christian Sensitivity of SARS-CoV-2 antibody tests with late convalescent sera |
title | Sensitivity of SARS-CoV-2 antibody tests with late convalescent sera |
title_full | Sensitivity of SARS-CoV-2 antibody tests with late convalescent sera |
title_fullStr | Sensitivity of SARS-CoV-2 antibody tests with late convalescent sera |
title_full_unstemmed | Sensitivity of SARS-CoV-2 antibody tests with late convalescent sera |
title_short | Sensitivity of SARS-CoV-2 antibody tests with late convalescent sera |
title_sort | sensitivity of sars-cov-2 antibody tests with late convalescent sera |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8372433/ https://www.ncbi.nlm.nih.gov/pubmed/35262021 http://dx.doi.org/10.1016/j.jcvp.2021.100038 |
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