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DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death
The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8373872/ https://www.ncbi.nlm.nih.gov/pubmed/34408182 http://dx.doi.org/10.1038/s41598-021-95976-7 |
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| author | Bonassi, Stefano Ceppi, Marcello Møller, Peter Azqueta, Amaya Milić, Mirta Neri, Monica Brunborg, Gunnar Godschalk, Roger Koppen, Gudrun Langie, Sabine A. S. Teixeira, João Paulo Bruzzone, Marco Da Silva, Juliana Benedetti, Danieli Cavallo, Delia Ursini, Cinzia Lucia Giovannelli, Lisa Moretti, Silvia Riso, Patrizia Del Bo’, Cristian Russo, Patrizia Dobrzyńska, Malgorzata Goroshinskaya, Irina A. Surikova, Ekaterina I. Staruchova, Marta Barančokova, Magdalena Volkovova, Katarina Kažimirova, Alena Smolkova, Bozena Laffon, Blanca Valdiglesias, Vanessa Pastor, Susana Marcos, Ricard Hernández, Alba Gajski, Goran Spremo-Potparević, Biljana Živković, Lada Boutet-Robinet, Elisa Perdry, Hervé Lebailly, Pierre Perez, Carlos L. Basaran, Nursen Nemeth, Zsuzsanna Safar, Anna Dusinska, Maria Collins, Andrew |
| author_facet | Bonassi, Stefano Ceppi, Marcello Møller, Peter Azqueta, Amaya Milić, Mirta Neri, Monica Brunborg, Gunnar Godschalk, Roger Koppen, Gudrun Langie, Sabine A. S. Teixeira, João Paulo Bruzzone, Marco Da Silva, Juliana Benedetti, Danieli Cavallo, Delia Ursini, Cinzia Lucia Giovannelli, Lisa Moretti, Silvia Riso, Patrizia Del Bo’, Cristian Russo, Patrizia Dobrzyńska, Malgorzata Goroshinskaya, Irina A. Surikova, Ekaterina I. Staruchova, Marta Barančokova, Magdalena Volkovova, Katarina Kažimirova, Alena Smolkova, Bozena Laffon, Blanca Valdiglesias, Vanessa Pastor, Susana Marcos, Ricard Hernández, Alba Gajski, Goran Spremo-Potparević, Biljana Živković, Lada Boutet-Robinet, Elisa Perdry, Hervé Lebailly, Pierre Perez, Carlos L. Basaran, Nursen Nemeth, Zsuzsanna Safar, Anna Dusinska, Maria Collins, Andrew |
| author_sort | Bonassi, Stefano |
| collection | PubMed |
| description | The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan–Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06–1.90) for overall mortality, and 1.94 (1.04–3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases. |
| format | Online Article Text |
| id | pubmed-8373872 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| publisher | Nature Publishing Group UK |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-83738722021-08-19 DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death Bonassi, Stefano Ceppi, Marcello Møller, Peter Azqueta, Amaya Milić, Mirta Neri, Monica Brunborg, Gunnar Godschalk, Roger Koppen, Gudrun Langie, Sabine A. S. Teixeira, João Paulo Bruzzone, Marco Da Silva, Juliana Benedetti, Danieli Cavallo, Delia Ursini, Cinzia Lucia Giovannelli, Lisa Moretti, Silvia Riso, Patrizia Del Bo’, Cristian Russo, Patrizia Dobrzyńska, Malgorzata Goroshinskaya, Irina A. Surikova, Ekaterina I. Staruchova, Marta Barančokova, Magdalena Volkovova, Katarina Kažimirova, Alena Smolkova, Bozena Laffon, Blanca Valdiglesias, Vanessa Pastor, Susana Marcos, Ricard Hernández, Alba Gajski, Goran Spremo-Potparević, Biljana Živković, Lada Boutet-Robinet, Elisa Perdry, Hervé Lebailly, Pierre Perez, Carlos L. Basaran, Nursen Nemeth, Zsuzsanna Safar, Anna Dusinska, Maria Collins, Andrew Sci Rep Article The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan–Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06–1.90) for overall mortality, and 1.94 (1.04–3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases. Nature Publishing Group UK 2021-08-18 /pmc/articles/PMC8373872/ /pubmed/34408182 http://dx.doi.org/10.1038/s41598-021-95976-7 Text en © The Author(s) 2021, corrected publication 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
| spellingShingle | Article Bonassi, Stefano Ceppi, Marcello Møller, Peter Azqueta, Amaya Milić, Mirta Neri, Monica Brunborg, Gunnar Godschalk, Roger Koppen, Gudrun Langie, Sabine A. S. Teixeira, João Paulo Bruzzone, Marco Da Silva, Juliana Benedetti, Danieli Cavallo, Delia Ursini, Cinzia Lucia Giovannelli, Lisa Moretti, Silvia Riso, Patrizia Del Bo’, Cristian Russo, Patrizia Dobrzyńska, Malgorzata Goroshinskaya, Irina A. Surikova, Ekaterina I. Staruchova, Marta Barančokova, Magdalena Volkovova, Katarina Kažimirova, Alena Smolkova, Bozena Laffon, Blanca Valdiglesias, Vanessa Pastor, Susana Marcos, Ricard Hernández, Alba Gajski, Goran Spremo-Potparević, Biljana Živković, Lada Boutet-Robinet, Elisa Perdry, Hervé Lebailly, Pierre Perez, Carlos L. Basaran, Nursen Nemeth, Zsuzsanna Safar, Anna Dusinska, Maria Collins, Andrew DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death |
| title | DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death |
| title_full | DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death |
| title_fullStr | DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death |
| title_full_unstemmed | DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death |
| title_short | DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death |
| title_sort | dna damage in circulating leukocytes measured with the comet assay may predict the risk of death |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8373872/ https://www.ncbi.nlm.nih.gov/pubmed/34408182 http://dx.doi.org/10.1038/s41598-021-95976-7 |
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