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Impact on Mechanical Properties of 10 versus 20 Minute Treatment of Human Pericardium with Glutaraldehyde in OZAKI Procedure

Purpose: The aim of this study was to analyze the effects of 10-minute (standard term) versus 20-minute treatment with glutaraldehyde (GA) on mechanical stability and physical strength of human pericardium in the setting of the OZAKI procedure. Methods: Leftover pericardium (6 patients) was bisected...

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Autores principales: Koechlin, Luca, Isu, Giuseppe, Borisov, Vladislav, Robles Diaz, Diana, Eckstein, Friedrich S., Marsano, Anna, Reuthebuch, Oliver
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Editorial Committee of Annals of Thoracic and Cardiovascular Surgery 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8374088/
https://www.ncbi.nlm.nih.gov/pubmed/33536387
http://dx.doi.org/10.5761/atcs.nm.20-00125
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author Koechlin, Luca
Isu, Giuseppe
Borisov, Vladislav
Robles Diaz, Diana
Eckstein, Friedrich S.
Marsano, Anna
Reuthebuch, Oliver
author_facet Koechlin, Luca
Isu, Giuseppe
Borisov, Vladislav
Robles Diaz, Diana
Eckstein, Friedrich S.
Marsano, Anna
Reuthebuch, Oliver
author_sort Koechlin, Luca
collection PubMed
description Purpose: The aim of this study was to analyze the effects of 10-minute (standard term) versus 20-minute treatment with glutaraldehyde (GA) on mechanical stability and physical strength of human pericardium in the setting of the OZAKI procedure. Methods: Leftover pericardium (6 patients) was bisected directly after the operation, and one-half was further fixed for 10 additional minutes. Uniaxial tensile tests were performed and ultimate tensile strength (UTS), ultimate tensile strain (uts), and collagen elastic modulus were evaluated. Results: Both treatments resulted in similar values of uniaxial stretching-generated elongations at rupture (10 minutes 25 ± 7 % vs. 20 minutes: 22 ± 5 %; p = 0.05), UTS (5.16 ± 2 MPa vs. 6.54 ± 3 MPa; p = 0.59), and collagen fiber stiffness (elastic modulus: 31.80 ± 15.05 MPa vs. 37.35 ± 15.78 MPa; p = 0.25). Conclusion: Prolongation of the fixation time of autologous pericardium has no significant effect on its mechanical stability; thus, extending the intraoperative treatment cannot be recommended.
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spelling pubmed-83740882021-08-19 Impact on Mechanical Properties of 10 versus 20 Minute Treatment of Human Pericardium with Glutaraldehyde in OZAKI Procedure Koechlin, Luca Isu, Giuseppe Borisov, Vladislav Robles Diaz, Diana Eckstein, Friedrich S. Marsano, Anna Reuthebuch, Oliver Ann Thorac Cardiovasc Surg New Methods Purpose: The aim of this study was to analyze the effects of 10-minute (standard term) versus 20-minute treatment with glutaraldehyde (GA) on mechanical stability and physical strength of human pericardium in the setting of the OZAKI procedure. Methods: Leftover pericardium (6 patients) was bisected directly after the operation, and one-half was further fixed for 10 additional minutes. Uniaxial tensile tests were performed and ultimate tensile strength (UTS), ultimate tensile strain (uts), and collagen elastic modulus were evaluated. Results: Both treatments resulted in similar values of uniaxial stretching-generated elongations at rupture (10 minutes 25 ± 7 % vs. 20 minutes: 22 ± 5 %; p = 0.05), UTS (5.16 ± 2 MPa vs. 6.54 ± 3 MPa; p = 0.59), and collagen fiber stiffness (elastic modulus: 31.80 ± 15.05 MPa vs. 37.35 ± 15.78 MPa; p = 0.25). Conclusion: Prolongation of the fixation time of autologous pericardium has no significant effect on its mechanical stability; thus, extending the intraoperative treatment cannot be recommended. The Editorial Committee of Annals of Thoracic and Cardiovascular Surgery 2021-02-03 2021 /pmc/articles/PMC8374088/ /pubmed/33536387 http://dx.doi.org/10.5761/atcs.nm.20-00125 Text en ©2021 Annals of Thoracic and Cardiovascular Surgery https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NonDerivatives International License (https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle New Methods
Koechlin, Luca
Isu, Giuseppe
Borisov, Vladislav
Robles Diaz, Diana
Eckstein, Friedrich S.
Marsano, Anna
Reuthebuch, Oliver
Impact on Mechanical Properties of 10 versus 20 Minute Treatment of Human Pericardium with Glutaraldehyde in OZAKI Procedure
title Impact on Mechanical Properties of 10 versus 20 Minute Treatment of Human Pericardium with Glutaraldehyde in OZAKI Procedure
title_full Impact on Mechanical Properties of 10 versus 20 Minute Treatment of Human Pericardium with Glutaraldehyde in OZAKI Procedure
title_fullStr Impact on Mechanical Properties of 10 versus 20 Minute Treatment of Human Pericardium with Glutaraldehyde in OZAKI Procedure
title_full_unstemmed Impact on Mechanical Properties of 10 versus 20 Minute Treatment of Human Pericardium with Glutaraldehyde in OZAKI Procedure
title_short Impact on Mechanical Properties of 10 versus 20 Minute Treatment of Human Pericardium with Glutaraldehyde in OZAKI Procedure
title_sort impact on mechanical properties of 10 versus 20 minute treatment of human pericardium with glutaraldehyde in ozaki procedure
topic New Methods
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8374088/
https://www.ncbi.nlm.nih.gov/pubmed/33536387
http://dx.doi.org/10.5761/atcs.nm.20-00125
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