A method to establish a chronic restraint stress mouse model with colorectal cancer xenografts

Colorectal cancer (CRC) remains one of the most common clinical cancers of digestive tract. Recently, a large number of researches have shown that chronic stress can actively participate in the development of CRC. The proposed method successfully established the model of chronic stress mouse model with colorectal cancer. • Chronic restraint stress (CRS) was used to establish chronic stress model. • CRS was combined with a colorectal cancer xenografts model. • Behavioural tests and tumour growth were used to evaluate model construction.