Iodine-based contrast staining improves micro-computed tomography of atherosclerotic coronary arteries

We sought to develop a reversible staining protocol using micro-computed tomography (micro-CT) paired with a radiopaque contrast agent that allows for three-dimensional in situ • Iohexol enhances ex vivo micro-CT imaging of atherosclerotic coronary arteries; • Iohexol allows for improved tissue segm...

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Autores principales: Self, Trevor S., Ginn-Hedman, Anne-Marie, Newell-Fugate, Annie E., Weeks, Brad R., Heaps, Cristine L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Method Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8374297/
https://www.ncbi.nlm.nih.gov/pubmed/34434817
http://dx.doi.org/10.1016/j.mex.2021.101297
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author Self, Trevor S.
Ginn-Hedman, Anne-Marie
Newell-Fugate, Annie E.
Weeks, Brad R.
Heaps, Cristine L.
author_facet Self, Trevor S.
Ginn-Hedman, Anne-Marie
Newell-Fugate, Annie E.
Weeks, Brad R.
Heaps, Cristine L.
author_sort Self, Trevor S.
collection PubMed
description We sought to develop a reversible staining protocol using micro-computed tomography (micro-CT) paired with a radiopaque contrast agent that allows for three-dimensional in situ • Iohexol enhances ex vivo micro-CT imaging of atherosclerotic coronary arteries; • Iohexol allows for improved tissue segmentation during micro-CT image analysis; • Effectiveness of iohexol penetration of the tissue was dependent on concentration and duration of incubation.
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spelling pubmed-83742972021-08-24 Iodine-based contrast staining improves micro-computed tomography of atherosclerotic coronary arteries Self, Trevor S. Ginn-Hedman, Anne-Marie Newell-Fugate, Annie E. Weeks, Brad R. Heaps, Cristine L. MethodsX Method Article We sought to develop a reversible staining protocol using micro-computed tomography (micro-CT) paired with a radiopaque contrast agent that allows for three-dimensional in situ • Iohexol enhances ex vivo micro-CT imaging of atherosclerotic coronary arteries; • Iohexol allows for improved tissue segmentation during micro-CT image analysis; • Effectiveness of iohexol penetration of the tissue was dependent on concentration and duration of incubation. Elsevier 2021-03-05 /pmc/articles/PMC8374297/ /pubmed/34434817 http://dx.doi.org/10.1016/j.mex.2021.101297 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Method Article
Self, Trevor S.
Ginn-Hedman, Anne-Marie
Newell-Fugate, Annie E.
Weeks, Brad R.
Heaps, Cristine L.
Iodine-based contrast staining improves micro-computed tomography of atherosclerotic coronary arteries
title Iodine-based contrast staining improves micro-computed tomography of atherosclerotic coronary arteries
title_full Iodine-based contrast staining improves micro-computed tomography of atherosclerotic coronary arteries
title_fullStr Iodine-based contrast staining improves micro-computed tomography of atherosclerotic coronary arteries
title_full_unstemmed Iodine-based contrast staining improves micro-computed tomography of atherosclerotic coronary arteries
title_short Iodine-based contrast staining improves micro-computed tomography of atherosclerotic coronary arteries
title_sort iodine-based contrast staining improves micro-computed tomography of atherosclerotic coronary arteries
topic Method Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8374297/
https://www.ncbi.nlm.nih.gov/pubmed/34434817
http://dx.doi.org/10.1016/j.mex.2021.101297
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