Determination of arsenic chemical species in rice grains using high-performance liquid chromatography coupled to hydride generator with atomic fluorescence detector (HPLC-HG-AFS)

The objective of this work is the validation and implementation of an analytical method for the determination of arsenic chemical species in rice grain samples using High-performance liquid chromatography coupled to a hydride generator with atomic fluorescence detector (HPLC-HG-AFS). The extraction protocol developed was based on HNO(3) 0.28 M (90 °C, 2 h), microwave-assisted. The results showed recovery percentages of arsenite (As (III)) (99–101%), arsenate (As (V)) (91–96%), dimethylarsinic acid (DMA) (92–102%) and monomethylarsonic acid (MMA) (94–97%). The precision of the method presented coefficients of variation lower than 7% and 8% for repeatability and reproducibility respectively. The detection limits were 2.5, 3.75, 7.5 and 4.0 µg kg(−1) • The extraction protocol developed was based on Microwave-assisted acid extraction. • This methodology offers good sensitivity, precision, accuracy, detection and quantification limits. • It was successfully applied to determination of arsenic chemical species in rice grains.