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A method for the transient inhibition of toxicity of secretory recombinant proteins, exemplified by bacterial alkaline phosphatase. Novel protocol for problematic DNA termini dephosphorylation

Genes encoding proteins ‘toxic' to recombinant host are difficult for cloning/expression and recombinant clones are unstable. Even tightly controlled inducible T7-lac, P(BAD), P(L), P(R) promoters are not totally silent in an uninduced state and thus not adequate for highly toxic proteins. An i...

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Detalles Bibliográficos
Autores principales:	Krawczun, Natalia, Bielawa, Marta, Szemiako, Kasjan, Łubkowska, Beata, Sobolewski, Ireneusz, Zylicz-Stachula, Agnieszka, Skowron, Piotr M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Method Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8374387/ https://www.ncbi.nlm.nih.gov/pubmed/34430244 http://dx.doi.org/10.1016/j.mex.2021.101340

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