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Validation of gene editing efficiency with CRISPR-Cas9 system directly in rat zygotes using electroporation mediated delivery and embryo culture

Successful use of the CRISPR-Cas9 system for gene manipulation relies on identifying effective and efficient guide RNA sequences (gRNAs). When the goal is to create transgenic animal/rodent models by knocking-in desired sequences using homology-directed repair (HDR), selecting effective guides becom...

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Detalles Bibliográficos
Autores principales:	Challa, Anil K., Stanford, Denise, Allen, Antonio, Rasmussen, Lawrence, Amanor, Ferdinand K., Raju, S. Vamsee
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Method Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8374522/ https://www.ncbi.nlm.nih.gov/pubmed/34430314 http://dx.doi.org/10.1016/j.mex.2021.101419

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