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Differential development and electrophysiological activity in cultured cortical neurons from the mouse and cynomolgus monkey

In vitro cultures of primary cortical neurons are widely used to investigate neuronal function. However, it has yet to be fully investigated whether there are significant differences in development and function between cultured rodent and primate cortical neurons, and whether these differences influ...

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Autores principales: Zhang, Xue-Yan, Li, Jun, Li, Cai-Juan, Lin, Ying-Qi, Huang, Chun-Hui, Zheng, Xiao, Song, Xi-Chen, Tu, Zhu-Chi, Li, Xiao-Jiang, Yan, Sen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8374592/
https://www.ncbi.nlm.nih.gov/pubmed/33907033
http://dx.doi.org/10.4103/1673-5374.313056
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author Zhang, Xue-Yan
Li, Jun
Li, Cai-Juan
Lin, Ying-Qi
Huang, Chun-Hui
Zheng, Xiao
Song, Xi-Chen
Tu, Zhu-Chi
Li, Xiao-Jiang
Yan, Sen
author_facet Zhang, Xue-Yan
Li, Jun
Li, Cai-Juan
Lin, Ying-Qi
Huang, Chun-Hui
Zheng, Xiao
Song, Xi-Chen
Tu, Zhu-Chi
Li, Xiao-Jiang
Yan, Sen
author_sort Zhang, Xue-Yan
collection PubMed
description In vitro cultures of primary cortical neurons are widely used to investigate neuronal function. However, it has yet to be fully investigated whether there are significant differences in development and function between cultured rodent and primate cortical neurons, and whether these differences influence the utilization of cultured cortical neurons to model pathological conditions. Using in vitro culture techniques combined with immunofluorescence and electrophysiological methods, our study found that the development and maturation of primary cerebral cortical neurons from cynomolgus monkeys were slower than those from mice. We used a microelectrode array technique to compare the electrophysiological differences in cortical neurons, and found that primary cortical neurons from the mouse brain began to show electrical activity earlier than those from the cynomolgus monkey. Although cultured monkey cortical neurons developed slowly in vitro, they exhibited typical pathological features-revealed by immunofluorescent staining-when infected with adeno-associated viral vectors expressing mutant huntingtin (HTT), the Huntington's disease protein. A quantitative analysis of the cultured monkey cortical neurons also confirmed that mutant HTT significantly reduced the length of neurites. Therefore, compared with the primary cortical neurons of mice, cultured monkey cortical neurons have longer developmental and survival times and greater sustained physiological activity, such as electrophysiological activity. Our findings also suggest that primary cynomolgus monkey neurons cultured in vitro can simulate a cell model of human neurodegenerative disease, and may be useful for investigating time-dependent neuronal death as well as treatment via neuronal regeneration. All mouse experiments and protocols were approved by the Animal Care and Use Committee of Jinan University of China (IACUC Approval No. 20200512-04) on May 12, 2020. All monkey experiments were approved by the IACUC protocol (IACUC Approval No. LDACU 20190820-01) on August 23, 2019 for animal management and use.
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spelling pubmed-83745922021-08-25 Differential development and electrophysiological activity in cultured cortical neurons from the mouse and cynomolgus monkey Zhang, Xue-Yan Li, Jun Li, Cai-Juan Lin, Ying-Qi Huang, Chun-Hui Zheng, Xiao Song, Xi-Chen Tu, Zhu-Chi Li, Xiao-Jiang Yan, Sen Neural Regen Res Research Article In vitro cultures of primary cortical neurons are widely used to investigate neuronal function. However, it has yet to be fully investigated whether there are significant differences in development and function between cultured rodent and primate cortical neurons, and whether these differences influence the utilization of cultured cortical neurons to model pathological conditions. Using in vitro culture techniques combined with immunofluorescence and electrophysiological methods, our study found that the development and maturation of primary cerebral cortical neurons from cynomolgus monkeys were slower than those from mice. We used a microelectrode array technique to compare the electrophysiological differences in cortical neurons, and found that primary cortical neurons from the mouse brain began to show electrical activity earlier than those from the cynomolgus monkey. Although cultured monkey cortical neurons developed slowly in vitro, they exhibited typical pathological features-revealed by immunofluorescent staining-when infected with adeno-associated viral vectors expressing mutant huntingtin (HTT), the Huntington's disease protein. A quantitative analysis of the cultured monkey cortical neurons also confirmed that mutant HTT significantly reduced the length of neurites. Therefore, compared with the primary cortical neurons of mice, cultured monkey cortical neurons have longer developmental and survival times and greater sustained physiological activity, such as electrophysiological activity. Our findings also suggest that primary cynomolgus monkey neurons cultured in vitro can simulate a cell model of human neurodegenerative disease, and may be useful for investigating time-dependent neuronal death as well as treatment via neuronal regeneration. All mouse experiments and protocols were approved by the Animal Care and Use Committee of Jinan University of China (IACUC Approval No. 20200512-04) on May 12, 2020. All monkey experiments were approved by the IACUC protocol (IACUC Approval No. LDACU 20190820-01) on August 23, 2019 for animal management and use. Wolters Kluwer - Medknow 2021-04-23 /pmc/articles/PMC8374592/ /pubmed/33907033 http://dx.doi.org/10.4103/1673-5374.313056 Text en Copyright: © Neural Regeneration Research https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Research Article
Zhang, Xue-Yan
Li, Jun
Li, Cai-Juan
Lin, Ying-Qi
Huang, Chun-Hui
Zheng, Xiao
Song, Xi-Chen
Tu, Zhu-Chi
Li, Xiao-Jiang
Yan, Sen
Differential development and electrophysiological activity in cultured cortical neurons from the mouse and cynomolgus monkey
title Differential development and electrophysiological activity in cultured cortical neurons from the mouse and cynomolgus monkey
title_full Differential development and electrophysiological activity in cultured cortical neurons from the mouse and cynomolgus monkey
title_fullStr Differential development and electrophysiological activity in cultured cortical neurons from the mouse and cynomolgus monkey
title_full_unstemmed Differential development and electrophysiological activity in cultured cortical neurons from the mouse and cynomolgus monkey
title_short Differential development and electrophysiological activity in cultured cortical neurons from the mouse and cynomolgus monkey
title_sort differential development and electrophysiological activity in cultured cortical neurons from the mouse and cynomolgus monkey
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8374592/
https://www.ncbi.nlm.nih.gov/pubmed/33907033
http://dx.doi.org/10.4103/1673-5374.313056
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