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Criibacterium bergeronii gen. nov., sp. nov., a new member of the family Peptostreptococcaceae, isolated from human clinical samples

A rod-shaped, motile anaerobic bacterium, designated CCRI-22567(T), was isolated from a vaginal sample of a woman diagnosed with bacterial vaginosis and subjected to a polyphasic taxonomic study. The novel strain was capable of growth at 30–42 °C (optimum, 42 °C), at pH 5.5–8.5 (optimum, pH 7.0–7.5)...

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Autores principales: Maheux, Andrée F., Boudreau, Dominique K., Abed, Jehane Y., Bérubé, Ève, Brodeur, Stéphanie, Bernard, Kathryn A., Hashimi, Ameena, Ducrey, Éloïse, Guay, Émilie F., Raymond, Frédéric, Corbeil, Jacques, Domingo, Marc-Christian, Roy, Paul H., Boissinot, Maurice, Tocheva, Elitza I., Omar, Rabeea F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8375425/
https://www.ncbi.nlm.nih.gov/pubmed/33586648
http://dx.doi.org/10.1099/ijsem.0.004691
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author Maheux, Andrée F.
Boudreau, Dominique K.
Abed, Jehane Y.
Bérubé, Ève
Brodeur, Stéphanie
Bernard, Kathryn A.
Hashimi, Ameena
Ducrey, Éloïse
Guay, Émilie F.
Raymond, Frédéric
Corbeil, Jacques
Domingo, Marc-Christian
Roy, Paul H.
Boissinot, Maurice
Tocheva, Elitza I.
Omar, Rabeea F.
author_facet Maheux, Andrée F.
Boudreau, Dominique K.
Abed, Jehane Y.
Bérubé, Ève
Brodeur, Stéphanie
Bernard, Kathryn A.
Hashimi, Ameena
Ducrey, Éloïse
Guay, Émilie F.
Raymond, Frédéric
Corbeil, Jacques
Domingo, Marc-Christian
Roy, Paul H.
Boissinot, Maurice
Tocheva, Elitza I.
Omar, Rabeea F.
author_sort Maheux, Andrée F.
collection PubMed
description A rod-shaped, motile anaerobic bacterium, designated CCRI-22567(T), was isolated from a vaginal sample of a woman diagnosed with bacterial vaginosis and subjected to a polyphasic taxonomic study. The novel strain was capable of growth at 30–42 °C (optimum, 42 °C), at pH 5.5–8.5 (optimum, pH 7.0–7.5) and in the presence of 0–1.5 % (w/v) NaCl (optimally at 0.5 % NaCl). The phylogenetic trees based on 16S rRNA gene sequences showed that strain CCRI-22567(T) forms a distinct evolutionary lineage independent of other taxa in the family Peptostreptococcaceae. Strain CCRI-22567(T) exhibited 90.1 % 16S rRNA gene sequence similarity to Peptoanaerobacter stomatis ACC19a(T) and 89.7 % to Eubacterium yurii subsp. schtitka ATCC 43716. The three closest organisms with an available whole genome were compared to strain CCRI-22567(T) for genomic relatedness assessment. The genomic average nucleotide identities (OrthoANIu) obtained with Peptoanaerobacter stomatis ACC19a(T), Eubacterium yurii subsp. margaretiae ATCC 43715 and Filifactor alocis ATCC 35896(T) were 71.8, 70.3 and 69.6 %, respectively. Strain CCRI-22567(T) contained C(18 : 1) ω9c and C(18 : 1) ω9c DMA as the major fatty acids. The DNA G+C content of strain CCRI-22567(T) based on its genome sequence was 33.8 mol%. On the basis of the phylogenetic, chemotaxonomic and other phenotypic properties, strain CCRI-22567(T) is considered to represent a new genus and species within the family Peptostreptococcaceae, for which the name Criibacterium bergeronii gen. nov., sp. nov., is proposed. The type strain of Criibacterium bergeronii is CCRI-22567(T) (=LMG 31278(T)=DSM 107614(T)=CCUG 72594(T)).
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spelling pubmed-83754252021-08-20 Criibacterium bergeronii gen. nov., sp. nov., a new member of the family Peptostreptococcaceae, isolated from human clinical samples Maheux, Andrée F. Boudreau, Dominique K. Abed, Jehane Y. Bérubé, Ève Brodeur, Stéphanie Bernard, Kathryn A. Hashimi, Ameena Ducrey, Éloïse Guay, Émilie F. Raymond, Frédéric Corbeil, Jacques Domingo, Marc-Christian Roy, Paul H. Boissinot, Maurice Tocheva, Elitza I. Omar, Rabeea F. Int J Syst Evol Microbiol New Taxa A rod-shaped, motile anaerobic bacterium, designated CCRI-22567(T), was isolated from a vaginal sample of a woman diagnosed with bacterial vaginosis and subjected to a polyphasic taxonomic study. The novel strain was capable of growth at 30–42 °C (optimum, 42 °C), at pH 5.5–8.5 (optimum, pH 7.0–7.5) and in the presence of 0–1.5 % (w/v) NaCl (optimally at 0.5 % NaCl). The phylogenetic trees based on 16S rRNA gene sequences showed that strain CCRI-22567(T) forms a distinct evolutionary lineage independent of other taxa in the family Peptostreptococcaceae. Strain CCRI-22567(T) exhibited 90.1 % 16S rRNA gene sequence similarity to Peptoanaerobacter stomatis ACC19a(T) and 89.7 % to Eubacterium yurii subsp. schtitka ATCC 43716. The three closest organisms with an available whole genome were compared to strain CCRI-22567(T) for genomic relatedness assessment. The genomic average nucleotide identities (OrthoANIu) obtained with Peptoanaerobacter stomatis ACC19a(T), Eubacterium yurii subsp. margaretiae ATCC 43715 and Filifactor alocis ATCC 35896(T) were 71.8, 70.3 and 69.6 %, respectively. Strain CCRI-22567(T) contained C(18 : 1) ω9c and C(18 : 1) ω9c DMA as the major fatty acids. The DNA G+C content of strain CCRI-22567(T) based on its genome sequence was 33.8 mol%. On the basis of the phylogenetic, chemotaxonomic and other phenotypic properties, strain CCRI-22567(T) is considered to represent a new genus and species within the family Peptostreptococcaceae, for which the name Criibacterium bergeronii gen. nov., sp. nov., is proposed. The type strain of Criibacterium bergeronii is CCRI-22567(T) (=LMG 31278(T)=DSM 107614(T)=CCUG 72594(T)). Microbiology Society 2021-02-15 /pmc/articles/PMC8375425/ /pubmed/33586648 http://dx.doi.org/10.1099/ijsem.0.004691 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
spellingShingle New Taxa
Maheux, Andrée F.
Boudreau, Dominique K.
Abed, Jehane Y.
Bérubé, Ève
Brodeur, Stéphanie
Bernard, Kathryn A.
Hashimi, Ameena
Ducrey, Éloïse
Guay, Émilie F.
Raymond, Frédéric
Corbeil, Jacques
Domingo, Marc-Christian
Roy, Paul H.
Boissinot, Maurice
Tocheva, Elitza I.
Omar, Rabeea F.
Criibacterium bergeronii gen. nov., sp. nov., a new member of the family Peptostreptococcaceae, isolated from human clinical samples
title Criibacterium bergeronii gen. nov., sp. nov., a new member of the family Peptostreptococcaceae, isolated from human clinical samples
title_full Criibacterium bergeronii gen. nov., sp. nov., a new member of the family Peptostreptococcaceae, isolated from human clinical samples
title_fullStr Criibacterium bergeronii gen. nov., sp. nov., a new member of the family Peptostreptococcaceae, isolated from human clinical samples
title_full_unstemmed Criibacterium bergeronii gen. nov., sp. nov., a new member of the family Peptostreptococcaceae, isolated from human clinical samples
title_short Criibacterium bergeronii gen. nov., sp. nov., a new member of the family Peptostreptococcaceae, isolated from human clinical samples
title_sort criibacterium bergeronii gen. nov., sp. nov., a new member of the family peptostreptococcaceae, isolated from human clinical samples
topic New Taxa
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8375425/
https://www.ncbi.nlm.nih.gov/pubmed/33586648
http://dx.doi.org/10.1099/ijsem.0.004691
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