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Motilimonas cestriensis sp. nov., isolated from an inland brine spring in Northern England
A novel slightly halophilic Gram-stain-negative bacterial strain (MKS20(T)) was isolated from a brine sample collected from one of the Anderton brine springs in the Cheshire salt district, located in Northern England. Phylogenetic analysis of the 16S rRNA gene sequence revealed a close proximity to...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Microbiology Society
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8375427/ https://www.ncbi.nlm.nih.gov/pubmed/33739249 http://dx.doi.org/10.1099/ijsem.0.004763 |
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author | Kelbrick, Matthew Abed, Raeid M. M. Antunes, André |
author_facet | Kelbrick, Matthew Abed, Raeid M. M. Antunes, André |
author_sort | Kelbrick, Matthew |
collection | PubMed |
description | A novel slightly halophilic Gram-stain-negative bacterial strain (MKS20(T)) was isolated from a brine sample collected from one of the Anderton brine springs in the Cheshire salt district, located in Northern England. Phylogenetic analysis of the 16S rRNA gene sequence revealed a close proximity to Motilimonas eburnea (98.30 %), followed by Motilimonas pumila (96.62 %), the two currently described species within the genus Motilimonas. Strain MKS20(T) forms white-beige-pigmented colonies and grows optimally at 28–30 °C, in 1–3 % (w/v) NaCl and at pH 7–7.5. The strain was facultatively anaerobic and showed a broader range of carbohydrate use than other species in the genus Motilimonas. Q-8 was the sole respiratory quinone and the major fatty acids (>10 %) were summed feature 3 (C(16 : 1) ω6c and/or C(16 : 1) ω7c) and C(16 : 0). The polar lipid profile included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidyglycerol and several unidentified lipids. The G+C content of the genomic DNA was 44.2 mol%. Average nucleotide identity and DNA–DNA hybridization data were consistent with assignment to a separate species. Based on the phylogenetic and genomic-based analyses, as well as physiological and biochemical characteristics, we propose that strain MKS20(T) (=DSM 109936(T), MCCC 1K04071(T)) represents a new species of the genus Motilimonas, with the name Motilimonas cestriensis sp. nov. |
format | Online Article Text |
id | pubmed-8375427 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Microbiology Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-83754272021-08-20 Motilimonas cestriensis sp. nov., isolated from an inland brine spring in Northern England Kelbrick, Matthew Abed, Raeid M. M. Antunes, André Int J Syst Evol Microbiol New Taxa A novel slightly halophilic Gram-stain-negative bacterial strain (MKS20(T)) was isolated from a brine sample collected from one of the Anderton brine springs in the Cheshire salt district, located in Northern England. Phylogenetic analysis of the 16S rRNA gene sequence revealed a close proximity to Motilimonas eburnea (98.30 %), followed by Motilimonas pumila (96.62 %), the two currently described species within the genus Motilimonas. Strain MKS20(T) forms white-beige-pigmented colonies and grows optimally at 28–30 °C, in 1–3 % (w/v) NaCl and at pH 7–7.5. The strain was facultatively anaerobic and showed a broader range of carbohydrate use than other species in the genus Motilimonas. Q-8 was the sole respiratory quinone and the major fatty acids (>10 %) were summed feature 3 (C(16 : 1) ω6c and/or C(16 : 1) ω7c) and C(16 : 0). The polar lipid profile included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidyglycerol and several unidentified lipids. The G+C content of the genomic DNA was 44.2 mol%. Average nucleotide identity and DNA–DNA hybridization data were consistent with assignment to a separate species. Based on the phylogenetic and genomic-based analyses, as well as physiological and biochemical characteristics, we propose that strain MKS20(T) (=DSM 109936(T), MCCC 1K04071(T)) represents a new species of the genus Motilimonas, with the name Motilimonas cestriensis sp. nov. Microbiology Society 2021-03-19 /pmc/articles/PMC8375427/ /pubmed/33739249 http://dx.doi.org/10.1099/ijsem.0.004763 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License. |
spellingShingle | New Taxa Kelbrick, Matthew Abed, Raeid M. M. Antunes, André Motilimonas cestriensis sp. nov., isolated from an inland brine spring in Northern England |
title | Motilimonas cestriensis sp. nov., isolated from an inland brine spring in Northern England |
title_full | Motilimonas cestriensis sp. nov., isolated from an inland brine spring in Northern England |
title_fullStr | Motilimonas cestriensis sp. nov., isolated from an inland brine spring in Northern England |
title_full_unstemmed | Motilimonas cestriensis sp. nov., isolated from an inland brine spring in Northern England |
title_short | Motilimonas cestriensis sp. nov., isolated from an inland brine spring in Northern England |
title_sort | motilimonas cestriensis sp. nov., isolated from an inland brine spring in northern england |
topic | New Taxa |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8375427/ https://www.ncbi.nlm.nih.gov/pubmed/33739249 http://dx.doi.org/10.1099/ijsem.0.004763 |
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