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Application of PCR-HRM method for microsatellite polymorphism genotyping in the LDHA gene of pigeons (Columba livia)

High-resolution melting (HRM) is a post-PCR method that allows to discriminate genotypes based on fluorescence changes during the melting phase. HRM is used to detect mutations or polymorphisms (e.g. microsatellites, SNPs, indels). Here, the (TTTAT)(3-5) microsatellite polymorphism within intron 6 o...

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Detalles Bibliográficos
Autores principales: Jedrzejczak-Silicka, Magdalena, Lepczynski, Adam, Gołębiowski, Filip, Dolata, Daniel, Dybus, Andrzej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8376019/
https://www.ncbi.nlm.nih.gov/pubmed/34411134
http://dx.doi.org/10.1371/journal.pone.0256065