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Pro-inflammatory cytokines and microRNAs in male infertility
BACKGROUND: Male infertility is a problem that affects 10–15% of men of reproductive age. In particular, gametogenesis is a complex process in which inflammation may play a central role through the secretion of cytokines and the expression of microRNAs. We assessed the potential role of proinflammat...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Netherlands
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8376712/ https://www.ncbi.nlm.nih.gov/pubmed/34319544 http://dx.doi.org/10.1007/s11033-021-06593-6 |
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author | Attia, Hana Finocchi, Federica Orciani, Monia Mehdi, Meriem Zidi Jrah, Ines Lazzarini, Raffaella Balercia, Giancarlo Mattioli Belmonte, Monica |
author_facet | Attia, Hana Finocchi, Federica Orciani, Monia Mehdi, Meriem Zidi Jrah, Ines Lazzarini, Raffaella Balercia, Giancarlo Mattioli Belmonte, Monica |
author_sort | Attia, Hana |
collection | PubMed |
description | BACKGROUND: Male infertility is a problem that affects 10–15% of men of reproductive age. In particular, gametogenesis is a complex process in which inflammation may play a central role through the secretion of cytokines and the expression of microRNAs. We assessed the potential role of proinflammatory cytokines (TNF-α, IL-6 and IL-1α) and microRNAs (miR-146a-5p, miR-34a-5p and miR-23a-3p) in the seminal plasma of infertile men compared to controls, evaluating their correlation with seminal and biochemical parameters. METHODS AND RESULTS: Expression of cytokines and microRNAs was analyzed by ELISA and q-PCR. Our data shows that IL-1α was significantly increased in the azoospermic group compared to controls, TNF-α mRNA was more expressed in the oligozoospermic group than controls. There were no significant differences in miRNAs expression among the three groups. The correlations between sperm parameters and inflammatory markers were evaluated, however no significance was highlighted. CONCLUSIONS: The determination of each inflammatory marker separately in the seminal plasma of subfertile men, despite some significant differences, does not have a diagnostic value in male infertility even if an assay of selective pro-inflammatory cytokines and microRNAs in the semen may improve the diagnosis of male infertility. |
format | Online Article Text |
id | pubmed-8376712 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-83767122021-09-02 Pro-inflammatory cytokines and microRNAs in male infertility Attia, Hana Finocchi, Federica Orciani, Monia Mehdi, Meriem Zidi Jrah, Ines Lazzarini, Raffaella Balercia, Giancarlo Mattioli Belmonte, Monica Mol Biol Rep Original Article BACKGROUND: Male infertility is a problem that affects 10–15% of men of reproductive age. In particular, gametogenesis is a complex process in which inflammation may play a central role through the secretion of cytokines and the expression of microRNAs. We assessed the potential role of proinflammatory cytokines (TNF-α, IL-6 and IL-1α) and microRNAs (miR-146a-5p, miR-34a-5p and miR-23a-3p) in the seminal plasma of infertile men compared to controls, evaluating their correlation with seminal and biochemical parameters. METHODS AND RESULTS: Expression of cytokines and microRNAs was analyzed by ELISA and q-PCR. Our data shows that IL-1α was significantly increased in the azoospermic group compared to controls, TNF-α mRNA was more expressed in the oligozoospermic group than controls. There were no significant differences in miRNAs expression among the three groups. The correlations between sperm parameters and inflammatory markers were evaluated, however no significance was highlighted. CONCLUSIONS: The determination of each inflammatory marker separately in the seminal plasma of subfertile men, despite some significant differences, does not have a diagnostic value in male infertility even if an assay of selective pro-inflammatory cytokines and microRNAs in the semen may improve the diagnosis of male infertility. Springer Netherlands 2021-07-28 2021 /pmc/articles/PMC8376712/ /pubmed/34319544 http://dx.doi.org/10.1007/s11033-021-06593-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Attia, Hana Finocchi, Federica Orciani, Monia Mehdi, Meriem Zidi Jrah, Ines Lazzarini, Raffaella Balercia, Giancarlo Mattioli Belmonte, Monica Pro-inflammatory cytokines and microRNAs in male infertility |
title | Pro-inflammatory cytokines and microRNAs in male infertility |
title_full | Pro-inflammatory cytokines and microRNAs in male infertility |
title_fullStr | Pro-inflammatory cytokines and microRNAs in male infertility |
title_full_unstemmed | Pro-inflammatory cytokines and microRNAs in male infertility |
title_short | Pro-inflammatory cytokines and microRNAs in male infertility |
title_sort | pro-inflammatory cytokines and micrornas in male infertility |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8376712/ https://www.ncbi.nlm.nih.gov/pubmed/34319544 http://dx.doi.org/10.1007/s11033-021-06593-6 |
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