Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries

The limited sensitivity of Förster Resonance Energy Transfer (FRET) biosensors hinders their broader applications. Here, we develop an approach integrating high-throughput FRET sorting and next-generation sequencing (FRET-Seq) to identify sensitive biosensors with varying substrate sequences from la...

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Detalles Bibliográficos
Autores principales: Liu, Longwei, Limsakul, Praopim, Meng, Xianhui, Huang, Yan, Harrison, Reed E. S., Huang, Tse-Shun, Shi, Yiwen, Yu, Yiyan, Charupanit, Krit, Zhong, Sheng, Lu, Shaoying, Zhang, Jin, Chien, Shu, Sun, Jie, Wang, Yingxiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8376904/
https://www.ncbi.nlm.nih.gov/pubmed/34413312
http://dx.doi.org/10.1038/s41467-021-25323-x

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