Cargando…

Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue

Tissue engineering principles allow the generation of functional tissues for biomedical applications. Reconstruction of large-scale bone defects with tissue-engineered bone has still not entered the clinical routine. In the present study, a bone substitute in combination with mesenchymal stem cells...

Descripción completa

Detalles Bibliográficos
Autores principales: Kengelbach-Weigand, Annika, Thielen, Carolina, Bäuerle, Tobias, Götzl, Rebekka, Gerber, Thomas, Körner, Carolin, Beier, Justus P., Horch, Raymund E., Boos, Anja M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377075/
https://www.ncbi.nlm.nih.gov/pubmed/34413320
http://dx.doi.org/10.1038/s41536-021-00158-8
Descripción
Sumario:Tissue engineering principles allow the generation of functional tissues for biomedical applications. Reconstruction of large-scale bone defects with tissue-engineered bone has still not entered the clinical routine. In the present study, a bone substitute in combination with mesenchymal stem cells (MSC) and endothelial progenitor cells (EPC) with or without growth factors BMP-2 and VEGF-A was prevascularized by an arteriovenous (AV) loop and transplanted into a critical-size tibia defect in the sheep model. With 3D imaging and immunohistochemistry, we could show that this approach is a feasible and simple alternative to the current clinical therapeutic option. This study serves as proof of concept for using large-scale transplantable, vascularized, and customizable bone, generated in a living organism for the reconstruction of load-bearing bone defects, individually tailored to the patient’s needs. With this approach in personalized medicine for the reconstruction of critical-size bone defects, regeneration of parts of the human body will become possible in the near future.