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Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue

Tissue engineering principles allow the generation of functional tissues for biomedical applications. Reconstruction of large-scale bone defects with tissue-engineered bone has still not entered the clinical routine. In the present study, a bone substitute in combination with mesenchymal stem cells...

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Autores principales: Kengelbach-Weigand, Annika, Thielen, Carolina, Bäuerle, Tobias, Götzl, Rebekka, Gerber, Thomas, Körner, Carolin, Beier, Justus P., Horch, Raymund E., Boos, Anja M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377075/
https://www.ncbi.nlm.nih.gov/pubmed/34413320
http://dx.doi.org/10.1038/s41536-021-00158-8
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author Kengelbach-Weigand, Annika
Thielen, Carolina
Bäuerle, Tobias
Götzl, Rebekka
Gerber, Thomas
Körner, Carolin
Beier, Justus P.
Horch, Raymund E.
Boos, Anja M.
author_facet Kengelbach-Weigand, Annika
Thielen, Carolina
Bäuerle, Tobias
Götzl, Rebekka
Gerber, Thomas
Körner, Carolin
Beier, Justus P.
Horch, Raymund E.
Boos, Anja M.
author_sort Kengelbach-Weigand, Annika
collection PubMed
description Tissue engineering principles allow the generation of functional tissues for biomedical applications. Reconstruction of large-scale bone defects with tissue-engineered bone has still not entered the clinical routine. In the present study, a bone substitute in combination with mesenchymal stem cells (MSC) and endothelial progenitor cells (EPC) with or without growth factors BMP-2 and VEGF-A was prevascularized by an arteriovenous (AV) loop and transplanted into a critical-size tibia defect in the sheep model. With 3D imaging and immunohistochemistry, we could show that this approach is a feasible and simple alternative to the current clinical therapeutic option. This study serves as proof of concept for using large-scale transplantable, vascularized, and customizable bone, generated in a living organism for the reconstruction of load-bearing bone defects, individually tailored to the patient’s needs. With this approach in personalized medicine for the reconstruction of critical-size bone defects, regeneration of parts of the human body will become possible in the near future.
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spelling pubmed-83770752021-09-08 Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue Kengelbach-Weigand, Annika Thielen, Carolina Bäuerle, Tobias Götzl, Rebekka Gerber, Thomas Körner, Carolin Beier, Justus P. Horch, Raymund E. Boos, Anja M. NPJ Regen Med Article Tissue engineering principles allow the generation of functional tissues for biomedical applications. Reconstruction of large-scale bone defects with tissue-engineered bone has still not entered the clinical routine. In the present study, a bone substitute in combination with mesenchymal stem cells (MSC) and endothelial progenitor cells (EPC) with or without growth factors BMP-2 and VEGF-A was prevascularized by an arteriovenous (AV) loop and transplanted into a critical-size tibia defect in the sheep model. With 3D imaging and immunohistochemistry, we could show that this approach is a feasible and simple alternative to the current clinical therapeutic option. This study serves as proof of concept for using large-scale transplantable, vascularized, and customizable bone, generated in a living organism for the reconstruction of load-bearing bone defects, individually tailored to the patient’s needs. With this approach in personalized medicine for the reconstruction of critical-size bone defects, regeneration of parts of the human body will become possible in the near future. Nature Publishing Group UK 2021-08-19 /pmc/articles/PMC8377075/ /pubmed/34413320 http://dx.doi.org/10.1038/s41536-021-00158-8 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Kengelbach-Weigand, Annika
Thielen, Carolina
Bäuerle, Tobias
Götzl, Rebekka
Gerber, Thomas
Körner, Carolin
Beier, Justus P.
Horch, Raymund E.
Boos, Anja M.
Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue
title Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue
title_full Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue
title_fullStr Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue
title_full_unstemmed Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue
title_short Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue
title_sort personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377075/
https://www.ncbi.nlm.nih.gov/pubmed/34413320
http://dx.doi.org/10.1038/s41536-021-00158-8
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