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Ultrasound-targeted microbubble destruction-mediated overexpression of Sirtuin 3 inhibits the progression of ovarian cancer

Ultrasound-targeted microbubble destruction (UTMD) has recently been developed as a promising noninvasive tool for organ- and tissue-specific gene or drug delivery. The aim of the present study was to explore the role of UTMD-mediated Sirtuin 3 (SIRT3) overexpression in the malignant behaviors of hu...

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Autores principales: Cheng, Li, Zhang, Dongmei, Yan, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377464/
https://www.ncbi.nlm.nih.gov/pubmed/34396428
http://dx.doi.org/10.3892/or.2021.8171
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author Cheng, Li
Zhang, Dongmei
Yan, Wei
author_facet Cheng, Li
Zhang, Dongmei
Yan, Wei
author_sort Cheng, Li
collection PubMed
description Ultrasound-targeted microbubble destruction (UTMD) has recently been developed as a promising noninvasive tool for organ- and tissue-specific gene or drug delivery. The aim of the present study was to explore the role of UTMD-mediated Sirtuin 3 (SIRT3) overexpression in the malignant behaviors of human ovarian cancer (HOC) cells. Reverse transcription-quantitative PCR was performed to detect SIRT3 mRNA expression levels in normal human ovarian epithelial cells and HOC cell lines; low SIRT3 expression was found in HOC cell lines, and the SKOV3 cell line was used in the following experiments. The SIRT3-microbubble (MB) was prepared, and the effects of ultrasound-treated SIRT3-MB on biological processes of SKOV3 cells were determined. The proliferation, migration, invasion and apoptosis of SKOV3 cells were measured after SIRT3 upregulation by UTMD. Xenograft tumors in nude mice were induced to observe tumor growth in vivo. Upregulation of SIRT3 inhibited the malignant behaviors of SKOV3 cells, whereas UTMD-mediated SIRT3 upregulation further inhibited proliferation, epithelial-mesenchymal transition, invasion and migration, and induced apoptosis of SKOV3 cells, and it also inhibited tumor formation and growth in vivo. Moreover, the present study identified hypoxia inducible factor-1α (HIF-1α) as a target of SIRT3. The present study provided evidence that UTMD-mediated overexpression of SIRT3 may suppress HOC progression through the inhibition of HIF-1α.
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spelling pubmed-83774642021-08-29 Ultrasound-targeted microbubble destruction-mediated overexpression of Sirtuin 3 inhibits the progression of ovarian cancer Cheng, Li Zhang, Dongmei Yan, Wei Oncol Rep Articles Ultrasound-targeted microbubble destruction (UTMD) has recently been developed as a promising noninvasive tool for organ- and tissue-specific gene or drug delivery. The aim of the present study was to explore the role of UTMD-mediated Sirtuin 3 (SIRT3) overexpression in the malignant behaviors of human ovarian cancer (HOC) cells. Reverse transcription-quantitative PCR was performed to detect SIRT3 mRNA expression levels in normal human ovarian epithelial cells and HOC cell lines; low SIRT3 expression was found in HOC cell lines, and the SKOV3 cell line was used in the following experiments. The SIRT3-microbubble (MB) was prepared, and the effects of ultrasound-treated SIRT3-MB on biological processes of SKOV3 cells were determined. The proliferation, migration, invasion and apoptosis of SKOV3 cells were measured after SIRT3 upregulation by UTMD. Xenograft tumors in nude mice were induced to observe tumor growth in vivo. Upregulation of SIRT3 inhibited the malignant behaviors of SKOV3 cells, whereas UTMD-mediated SIRT3 upregulation further inhibited proliferation, epithelial-mesenchymal transition, invasion and migration, and induced apoptosis of SKOV3 cells, and it also inhibited tumor formation and growth in vivo. Moreover, the present study identified hypoxia inducible factor-1α (HIF-1α) as a target of SIRT3. The present study provided evidence that UTMD-mediated overexpression of SIRT3 may suppress HOC progression through the inhibition of HIF-1α. D.A. Spandidos 2021-10 2021-08-12 /pmc/articles/PMC8377464/ /pubmed/34396428 http://dx.doi.org/10.3892/or.2021.8171 Text en Copyright: © Cheng et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Cheng, Li
Zhang, Dongmei
Yan, Wei
Ultrasound-targeted microbubble destruction-mediated overexpression of Sirtuin 3 inhibits the progression of ovarian cancer
title Ultrasound-targeted microbubble destruction-mediated overexpression of Sirtuin 3 inhibits the progression of ovarian cancer
title_full Ultrasound-targeted microbubble destruction-mediated overexpression of Sirtuin 3 inhibits the progression of ovarian cancer
title_fullStr Ultrasound-targeted microbubble destruction-mediated overexpression of Sirtuin 3 inhibits the progression of ovarian cancer
title_full_unstemmed Ultrasound-targeted microbubble destruction-mediated overexpression of Sirtuin 3 inhibits the progression of ovarian cancer
title_short Ultrasound-targeted microbubble destruction-mediated overexpression of Sirtuin 3 inhibits the progression of ovarian cancer
title_sort ultrasound-targeted microbubble destruction-mediated overexpression of sirtuin 3 inhibits the progression of ovarian cancer
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377464/
https://www.ncbi.nlm.nih.gov/pubmed/34396428
http://dx.doi.org/10.3892/or.2021.8171
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