Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver

The endoplasmic reticulum (ER) plays a central role in lipid homeostasis, but the role of individual ER subdomains in lipid biology has not been elucidated. WrappER is a curved wrapping type of rough-ER that establishes extensive contacts with almost every mitochondria of the hepatocyte in the mouse...

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Detalles Bibliográficos
Autores principales: Ilacqua, Nicolò, Anastasia, Irene, Pellegrini, Luca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377487/
https://www.ncbi.nlm.nih.gov/pubmed/34458870
http://dx.doi.org/10.1016/j.xpro.2021.100752
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author Ilacqua, Nicolò
Anastasia, Irene
Pellegrini, Luca
author_facet Ilacqua, Nicolò
Anastasia, Irene
Pellegrini, Luca
author_sort Ilacqua, Nicolò
collection PubMed
description The endoplasmic reticulum (ER) plays a central role in lipid homeostasis, but the role of individual ER subdomains in lipid biology has not been elucidated. WrappER is a curved wrapping type of rough-ER that establishes extensive contacts with almost every mitochondria of the hepatocyte in the mouse liver. Here, we describe a protocol for isolation of fractions enriched in wrappER-associated mitochondria from the mouse liver. We also provide techniques for assessing its quality by electron microscopy and biochemical/proteomic analysis. For complete information on the use and execution of this protocol, please refer to Anastasia et al. (2021).
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spelling pubmed-83774872021-08-26 Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver Ilacqua, Nicolò Anastasia, Irene Pellegrini, Luca STAR Protoc Protocol The endoplasmic reticulum (ER) plays a central role in lipid homeostasis, but the role of individual ER subdomains in lipid biology has not been elucidated. WrappER is a curved wrapping type of rough-ER that establishes extensive contacts with almost every mitochondria of the hepatocyte in the mouse liver. Here, we describe a protocol for isolation of fractions enriched in wrappER-associated mitochondria from the mouse liver. We also provide techniques for assessing its quality by electron microscopy and biochemical/proteomic analysis. For complete information on the use and execution of this protocol, please refer to Anastasia et al. (2021). Elsevier 2021-08-16 /pmc/articles/PMC8377487/ /pubmed/34458870 http://dx.doi.org/10.1016/j.xpro.2021.100752 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Ilacqua, Nicolò
Anastasia, Irene
Pellegrini, Luca
Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver
title Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver
title_full Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver
title_fullStr Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver
title_full_unstemmed Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver
title_short Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver
title_sort isolation and analysis of fractions enriched in wrapper-associated mitochondria from mouse liver
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377487/
https://www.ncbi.nlm.nih.gov/pubmed/34458870
http://dx.doi.org/10.1016/j.xpro.2021.100752
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