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A Newly Identified lncBCAS1-4_1 Associated With Vitamin D Signaling and EMT in Ovarian Cancer Cells

Long noncoding RNAs (lncRNAs) were identified rapidly due to their important role in many biological processes and human diseases including cancer. 1α,25-dihydroxyvitamin D3 [1α,25(OH)(2)D(3)] and its analogues are widely applied as preventative and therapeutic anticancer agents. However, the expres...

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Autores principales: Xue, Yaqi, Wang, Ping, Jiang, Fei, Yu, Jing, Ding, Hongmei, Zhang, Zengli, Pei, Hailong, Li, Bingyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377733/
https://www.ncbi.nlm.nih.gov/pubmed/34422647
http://dx.doi.org/10.3389/fonc.2021.691500
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author Xue, Yaqi
Wang, Ping
Jiang, Fei
Yu, Jing
Ding, Hongmei
Zhang, Zengli
Pei, Hailong
Li, Bingyan
author_facet Xue, Yaqi
Wang, Ping
Jiang, Fei
Yu, Jing
Ding, Hongmei
Zhang, Zengli
Pei, Hailong
Li, Bingyan
author_sort Xue, Yaqi
collection PubMed
description Long noncoding RNAs (lncRNAs) were identified rapidly due to their important role in many biological processes and human diseases including cancer. 1α,25-dihydroxyvitamin D3 [1α,25(OH)(2)D(3)] and its analogues are widely applied as preventative and therapeutic anticancer agents. However, the expression profile of lncRNAs regulated by 1α,25(OH)(2)D(3) in ovarian cancer remains to be clarified. In the present study, we found 606 lncRNAs and 102 mRNAs that showed differential expression (DE) based on microarray data. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that the DE genes were mainly enriched in TGF-β, MAPK, Ras, PI3K-Akt, and Hippo signaling pathways, as well as the vitamin D-related pathway. We further assessed the potential lncRNAs that linked vitamin D signaling with EMT, and lncBCAS1-4_1 was identified in the first time. Moreover, we found that the most upregulated lncBCAS1-4_1 showed 75% same transcripts with CYP24A1 (metabolic enzyme of 1α,25(OH)(2)D(3)). Finally, the lncBCAS1-4_1 gain-of-function cell model was established, which demonstrated that the knockdown of lncBCAS1-4_1 inhibited the proliferation and migration of ovarian cancer cells. Furthermore, lncBCAS1-4_1 could resist the antitumor effect of 1α,25(OH)(2)D(3), which was associated with upregulated ZEB1. These data provide new evidences that lncRNAs served as a target for the antitumor effect of 1α,25(OH)(2)D(3).
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spelling pubmed-83777332021-08-21 A Newly Identified lncBCAS1-4_1 Associated With Vitamin D Signaling and EMT in Ovarian Cancer Cells Xue, Yaqi Wang, Ping Jiang, Fei Yu, Jing Ding, Hongmei Zhang, Zengli Pei, Hailong Li, Bingyan Front Oncol Oncology Long noncoding RNAs (lncRNAs) were identified rapidly due to their important role in many biological processes and human diseases including cancer. 1α,25-dihydroxyvitamin D3 [1α,25(OH)(2)D(3)] and its analogues are widely applied as preventative and therapeutic anticancer agents. However, the expression profile of lncRNAs regulated by 1α,25(OH)(2)D(3) in ovarian cancer remains to be clarified. In the present study, we found 606 lncRNAs and 102 mRNAs that showed differential expression (DE) based on microarray data. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that the DE genes were mainly enriched in TGF-β, MAPK, Ras, PI3K-Akt, and Hippo signaling pathways, as well as the vitamin D-related pathway. We further assessed the potential lncRNAs that linked vitamin D signaling with EMT, and lncBCAS1-4_1 was identified in the first time. Moreover, we found that the most upregulated lncBCAS1-4_1 showed 75% same transcripts with CYP24A1 (metabolic enzyme of 1α,25(OH)(2)D(3)). Finally, the lncBCAS1-4_1 gain-of-function cell model was established, which demonstrated that the knockdown of lncBCAS1-4_1 inhibited the proliferation and migration of ovarian cancer cells. Furthermore, lncBCAS1-4_1 could resist the antitumor effect of 1α,25(OH)(2)D(3), which was associated with upregulated ZEB1. These data provide new evidences that lncRNAs served as a target for the antitumor effect of 1α,25(OH)(2)D(3). Frontiers Media S.A. 2021-08-05 /pmc/articles/PMC8377733/ /pubmed/34422647 http://dx.doi.org/10.3389/fonc.2021.691500 Text en Copyright © 2021 Xue, Wang, Jiang, Yu, Ding, Zhang, Pei and Li https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Xue, Yaqi
Wang, Ping
Jiang, Fei
Yu, Jing
Ding, Hongmei
Zhang, Zengli
Pei, Hailong
Li, Bingyan
A Newly Identified lncBCAS1-4_1 Associated With Vitamin D Signaling and EMT in Ovarian Cancer Cells
title A Newly Identified lncBCAS1-4_1 Associated With Vitamin D Signaling and EMT in Ovarian Cancer Cells
title_full A Newly Identified lncBCAS1-4_1 Associated With Vitamin D Signaling and EMT in Ovarian Cancer Cells
title_fullStr A Newly Identified lncBCAS1-4_1 Associated With Vitamin D Signaling and EMT in Ovarian Cancer Cells
title_full_unstemmed A Newly Identified lncBCAS1-4_1 Associated With Vitamin D Signaling and EMT in Ovarian Cancer Cells
title_short A Newly Identified lncBCAS1-4_1 Associated With Vitamin D Signaling and EMT in Ovarian Cancer Cells
title_sort newly identified lncbcas1-4_1 associated with vitamin d signaling and emt in ovarian cancer cells
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377733/
https://www.ncbi.nlm.nih.gov/pubmed/34422647
http://dx.doi.org/10.3389/fonc.2021.691500
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