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Induced pluripotent stem cells derived cardiomyocytes from Duchenne Muscular Dystrophy patients in vitro
OBJECTIVE: This study aimed at the in vitro generation of DMD-cardiomyocytes from patient-specific induced pluripotent stem cells derived from a Pakistani patient for future work on DMD in vitro disease modeling and drug testing for efficacy and toxicity. METHODS: This in vitro experimental study wa...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Professional Medical Publications
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377888/ https://www.ncbi.nlm.nih.gov/pubmed/34475915 http://dx.doi.org/10.12669/pjms.37.5.3104 |
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author | Ghori, Fareeha Faizan Wahid, Mohsin |
author_facet | Ghori, Fareeha Faizan Wahid, Mohsin |
author_sort | Ghori, Fareeha Faizan |
collection | PubMed |
description | OBJECTIVE: This study aimed at the in vitro generation of DMD-cardiomyocytes from patient-specific induced pluripotent stem cells derived from a Pakistani patient for future work on DMD in vitro disease modeling and drug testing for efficacy and toxicity. METHODS: This in vitro experimental study was carried out from December 2018 to January 2019 at Stem Cells and Regenerative Medicine Lab (SCRML) at Dow Research Institute of Biotechnology and Biomedical Sciences (DRIBBS), Dow University of Health Sciences (DUHS) Urine derived DMD-iPSCs were used which had been generated previously from a Pakistani DMD patient who had been selected through non-random purposive sampling. These were differentiated towards cardiomyocytes using Cardiomyocytes Differentiation media having specified growth factors and then the molecular characterization of the differentiated cells was done using immunofluorescence. RESULTS: Pakistani patient’s DMD-Cardiomyocytes were generated and their identity was confirmed by positive immunofluorescence for the expression of cardiac markers NKX2-5 and TNNT-2. CONCLUSION: This study aimed for in vitro generation of DMD cardiomyocytes for future application in disease modeling, new drug testing for efficacy and toxicity, as well as for drug-testing for tailored personalized therapy. To the best of our knowledge, this was the first time DMD-Cardiomyocytes were generated from Pakistani DMD patients using their own induced pluripotent stem cells. |
format | Online Article Text |
id | pubmed-8377888 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Professional Medical Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-83778882021-09-01 Induced pluripotent stem cells derived cardiomyocytes from Duchenne Muscular Dystrophy patients in vitro Ghori, Fareeha Faizan Wahid, Mohsin Pak J Med Sci Original Article OBJECTIVE: This study aimed at the in vitro generation of DMD-cardiomyocytes from patient-specific induced pluripotent stem cells derived from a Pakistani patient for future work on DMD in vitro disease modeling and drug testing for efficacy and toxicity. METHODS: This in vitro experimental study was carried out from December 2018 to January 2019 at Stem Cells and Regenerative Medicine Lab (SCRML) at Dow Research Institute of Biotechnology and Biomedical Sciences (DRIBBS), Dow University of Health Sciences (DUHS) Urine derived DMD-iPSCs were used which had been generated previously from a Pakistani DMD patient who had been selected through non-random purposive sampling. These were differentiated towards cardiomyocytes using Cardiomyocytes Differentiation media having specified growth factors and then the molecular characterization of the differentiated cells was done using immunofluorescence. RESULTS: Pakistani patient’s DMD-Cardiomyocytes were generated and their identity was confirmed by positive immunofluorescence for the expression of cardiac markers NKX2-5 and TNNT-2. CONCLUSION: This study aimed for in vitro generation of DMD cardiomyocytes for future application in disease modeling, new drug testing for efficacy and toxicity, as well as for drug-testing for tailored personalized therapy. To the best of our knowledge, this was the first time DMD-Cardiomyocytes were generated from Pakistani DMD patients using their own induced pluripotent stem cells. Professional Medical Publications 2021 /pmc/articles/PMC8377888/ /pubmed/34475915 http://dx.doi.org/10.12669/pjms.37.5.3104 Text en Copyright: © Pakistan Journal of Medical Sciences https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0 (https://creativecommons.org/licenses/by/3.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Ghori, Fareeha Faizan Wahid, Mohsin Induced pluripotent stem cells derived cardiomyocytes from Duchenne Muscular Dystrophy patients in vitro |
title | Induced pluripotent stem cells derived cardiomyocytes from Duchenne Muscular Dystrophy patients in vitro |
title_full | Induced pluripotent stem cells derived cardiomyocytes from Duchenne Muscular Dystrophy patients in vitro |
title_fullStr | Induced pluripotent stem cells derived cardiomyocytes from Duchenne Muscular Dystrophy patients in vitro |
title_full_unstemmed | Induced pluripotent stem cells derived cardiomyocytes from Duchenne Muscular Dystrophy patients in vitro |
title_short | Induced pluripotent stem cells derived cardiomyocytes from Duchenne Muscular Dystrophy patients in vitro |
title_sort | induced pluripotent stem cells derived cardiomyocytes from duchenne muscular dystrophy patients in vitro |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377888/ https://www.ncbi.nlm.nih.gov/pubmed/34475915 http://dx.doi.org/10.12669/pjms.37.5.3104 |
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