Cargando…
Environmental Surveillance of SARS-CoV-2 RNA in Wastewater and Groundwater in Quintana Roo, Mexico
The presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater has been reported as a result of fecal shedding of infected individuals. In this study, the occurrence of SARS-CoV-2 RNA was explored in primary-treated wastewater from two municipal wastewater treatment p...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8378111/ https://www.ncbi.nlm.nih.gov/pubmed/34415553 http://dx.doi.org/10.1007/s12560-021-09492-y |
_version_ | 1783740774027886592 |
---|---|
author | Rosiles-González, Gabriela Carrillo-Jovel, Victor Hugo Alzate-Gaviria, Liliana Betancourt, Walter Q. Gerba, Charles P. Moreno-Valenzuela, Oscar A. Tapia-Tussell, Raúl Hernández-Zepeda, Cecilia |
author_facet | Rosiles-González, Gabriela Carrillo-Jovel, Victor Hugo Alzate-Gaviria, Liliana Betancourt, Walter Q. Gerba, Charles P. Moreno-Valenzuela, Oscar A. Tapia-Tussell, Raúl Hernández-Zepeda, Cecilia |
author_sort | Rosiles-González, Gabriela |
collection | PubMed |
description | The presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater has been reported as a result of fecal shedding of infected individuals. In this study, the occurrence of SARS-CoV-2 RNA was explored in primary-treated wastewater from two municipal wastewater treatment plants in Quintana Roo, Mexico, along with groundwater from sinkholes, a household well, and submarine groundwater discharges. Physicochemical variables were obtained in situ, and coliphage densities were determined. Three virus concentration methods based on adsorption-elution and sequential filtration were used followed by RNA isolation. Quantification of SARS-CoV-2 was done by RT-qPCR using the CDC 2020 assay, 2019-nCoV_N1 and 2019-nCoV_N2. The Pepper mild mottle virus, one of the most abundant RNA viruses in wastewater was quantified by RT-qPCR and compared to SARS-CoV-2 concentrations. The use of three combined virus concentration methods together with two qPCR assays allowed the detection of SARS-CoV-2 RNA in 58% of the wastewater samples analyzed, whereas none of the groundwater samples were positive for SARS-CoV-2 RNA. Concentrations of SARS-CoV-2 in wastewater were from 1.8 × 10(3) to 7.5 × 10(3) genome copies per liter (GC l(−1)), using the N1 RT-qPCR assay, and from 2.4 × 10(2) to 5.9 × 10(3) GC l(−1) using the N2 RT-qPCR assay. Based on PMMoV prevalence detected in all wastewater and groundwater samples tested, the three viral concentration methods used could be successfully applied for SARS-CoV-2 RNA detection in further studies. This study represents the first detection of SARS-CoV-2 RNA in wastewater in southeast Mexico and provides a baseline for developing a wastewater-based epidemiology approach in the area. |
format | Online Article Text |
id | pubmed-8378111 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-83781112021-08-20 Environmental Surveillance of SARS-CoV-2 RNA in Wastewater and Groundwater in Quintana Roo, Mexico Rosiles-González, Gabriela Carrillo-Jovel, Victor Hugo Alzate-Gaviria, Liliana Betancourt, Walter Q. Gerba, Charles P. Moreno-Valenzuela, Oscar A. Tapia-Tussell, Raúl Hernández-Zepeda, Cecilia Food Environ Virol Original Paper The presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater has been reported as a result of fecal shedding of infected individuals. In this study, the occurrence of SARS-CoV-2 RNA was explored in primary-treated wastewater from two municipal wastewater treatment plants in Quintana Roo, Mexico, along with groundwater from sinkholes, a household well, and submarine groundwater discharges. Physicochemical variables were obtained in situ, and coliphage densities were determined. Three virus concentration methods based on adsorption-elution and sequential filtration were used followed by RNA isolation. Quantification of SARS-CoV-2 was done by RT-qPCR using the CDC 2020 assay, 2019-nCoV_N1 and 2019-nCoV_N2. The Pepper mild mottle virus, one of the most abundant RNA viruses in wastewater was quantified by RT-qPCR and compared to SARS-CoV-2 concentrations. The use of three combined virus concentration methods together with two qPCR assays allowed the detection of SARS-CoV-2 RNA in 58% of the wastewater samples analyzed, whereas none of the groundwater samples were positive for SARS-CoV-2 RNA. Concentrations of SARS-CoV-2 in wastewater were from 1.8 × 10(3) to 7.5 × 10(3) genome copies per liter (GC l(−1)), using the N1 RT-qPCR assay, and from 2.4 × 10(2) to 5.9 × 10(3) GC l(−1) using the N2 RT-qPCR assay. Based on PMMoV prevalence detected in all wastewater and groundwater samples tested, the three viral concentration methods used could be successfully applied for SARS-CoV-2 RNA detection in further studies. This study represents the first detection of SARS-CoV-2 RNA in wastewater in southeast Mexico and provides a baseline for developing a wastewater-based epidemiology approach in the area. Springer US 2021-08-20 2021 /pmc/articles/PMC8378111/ /pubmed/34415553 http://dx.doi.org/10.1007/s12560-021-09492-y Text en © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Paper Rosiles-González, Gabriela Carrillo-Jovel, Victor Hugo Alzate-Gaviria, Liliana Betancourt, Walter Q. Gerba, Charles P. Moreno-Valenzuela, Oscar A. Tapia-Tussell, Raúl Hernández-Zepeda, Cecilia Environmental Surveillance of SARS-CoV-2 RNA in Wastewater and Groundwater in Quintana Roo, Mexico |
title | Environmental Surveillance of SARS-CoV-2 RNA in Wastewater and Groundwater in Quintana Roo, Mexico |
title_full | Environmental Surveillance of SARS-CoV-2 RNA in Wastewater and Groundwater in Quintana Roo, Mexico |
title_fullStr | Environmental Surveillance of SARS-CoV-2 RNA in Wastewater and Groundwater in Quintana Roo, Mexico |
title_full_unstemmed | Environmental Surveillance of SARS-CoV-2 RNA in Wastewater and Groundwater in Quintana Roo, Mexico |
title_short | Environmental Surveillance of SARS-CoV-2 RNA in Wastewater and Groundwater in Quintana Roo, Mexico |
title_sort | environmental surveillance of sars-cov-2 rna in wastewater and groundwater in quintana roo, mexico |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8378111/ https://www.ncbi.nlm.nih.gov/pubmed/34415553 http://dx.doi.org/10.1007/s12560-021-09492-y |
work_keys_str_mv | AT rosilesgonzalezgabriela environmentalsurveillanceofsarscov2rnainwastewaterandgroundwaterinquintanaroomexico AT carrillojovelvictorhugo environmentalsurveillanceofsarscov2rnainwastewaterandgroundwaterinquintanaroomexico AT alzategavirialiliana environmentalsurveillanceofsarscov2rnainwastewaterandgroundwaterinquintanaroomexico AT betancourtwalterq environmentalsurveillanceofsarscov2rnainwastewaterandgroundwaterinquintanaroomexico AT gerbacharlesp environmentalsurveillanceofsarscov2rnainwastewaterandgroundwaterinquintanaroomexico AT morenovalenzuelaoscara environmentalsurveillanceofsarscov2rnainwastewaterandgroundwaterinquintanaroomexico AT tapiatussellraul environmentalsurveillanceofsarscov2rnainwastewaterandgroundwaterinquintanaroomexico AT hernandezzepedacecilia environmentalsurveillanceofsarscov2rnainwastewaterandgroundwaterinquintanaroomexico |