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Genetic Diversity Analysis of Surface-Related Antigen (SRA) in Plasmodium falciparum Imported From Africa to China

Plasmodium falciparum surface-related antigen (SRA) is located on the surfaces of gametocyte and merozoite and has the structural and functional characteristics of potential targets for multistage vaccine development. However, little information is available regarding the genetic polymorphism of pfs...

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Autores principales: Yang, Bo, Liu, Hong, Xu, Qin-Wen, Sun, Yi-Fan, Xu, Sui, Zhang, Hao, Tang, Jian-Xia, Zhu, Guo-Ding, Liu, Yao-Bao, Cao, Jun, Cheng, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8378275/
https://www.ncbi.nlm.nih.gov/pubmed/34421996
http://dx.doi.org/10.3389/fgene.2021.688606
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author Yang, Bo
Liu, Hong
Xu, Qin-Wen
Sun, Yi-Fan
Xu, Sui
Zhang, Hao
Tang, Jian-Xia
Zhu, Guo-Ding
Liu, Yao-Bao
Cao, Jun
Cheng, Yang
author_facet Yang, Bo
Liu, Hong
Xu, Qin-Wen
Sun, Yi-Fan
Xu, Sui
Zhang, Hao
Tang, Jian-Xia
Zhu, Guo-Ding
Liu, Yao-Bao
Cao, Jun
Cheng, Yang
author_sort Yang, Bo
collection PubMed
description Plasmodium falciparum surface-related antigen (SRA) is located on the surfaces of gametocyte and merozoite and has the structural and functional characteristics of potential targets for multistage vaccine development. However, little information is available regarding the genetic polymorphism of pfsra. To determine the extent of genetic variation about P. falciparum by characterizing the sra sequence, 74 P. falciparum samples were collected from migrant workers who returned to China from 12 countries of Africa between 2015 and 2019. The full length of the sra gene was amplified and sequenced. The average pairwise nucleotide diversities (π) of P. falciparum sra gene was 0.00132, and the haplotype diversity (Hd) was 0.770. The average number of nucleotide differences (k) for pfsra was 3.049. The ratio of non-synonymous (dN) to synonymous (dS) substitutions across sites (dN/dS) was 1.365. Amino acid substitutions of P. falciparum SRA could be categorized into 35 unique amino acid variants. Neutrality tests showed that the polymorphism of PfSRA was maintained by positive diversifying selection, which indicated its role as a potential target of protective immune responses and a vaccine candidate. Overall, the ability of the N-terminal of PfSRA antibodies to evoke inhibition of merozoite invasion of erythrocytes and conserved amino acid at low genetic diversity suggest that the N-terminal of PfSRA could be evaluated as a vaccine candidate against P. falciparum infection.
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spelling pubmed-83782752021-08-21 Genetic Diversity Analysis of Surface-Related Antigen (SRA) in Plasmodium falciparum Imported From Africa to China Yang, Bo Liu, Hong Xu, Qin-Wen Sun, Yi-Fan Xu, Sui Zhang, Hao Tang, Jian-Xia Zhu, Guo-Ding Liu, Yao-Bao Cao, Jun Cheng, Yang Front Genet Genetics Plasmodium falciparum surface-related antigen (SRA) is located on the surfaces of gametocyte and merozoite and has the structural and functional characteristics of potential targets for multistage vaccine development. However, little information is available regarding the genetic polymorphism of pfsra. To determine the extent of genetic variation about P. falciparum by characterizing the sra sequence, 74 P. falciparum samples were collected from migrant workers who returned to China from 12 countries of Africa between 2015 and 2019. The full length of the sra gene was amplified and sequenced. The average pairwise nucleotide diversities (π) of P. falciparum sra gene was 0.00132, and the haplotype diversity (Hd) was 0.770. The average number of nucleotide differences (k) for pfsra was 3.049. The ratio of non-synonymous (dN) to synonymous (dS) substitutions across sites (dN/dS) was 1.365. Amino acid substitutions of P. falciparum SRA could be categorized into 35 unique amino acid variants. Neutrality tests showed that the polymorphism of PfSRA was maintained by positive diversifying selection, which indicated its role as a potential target of protective immune responses and a vaccine candidate. Overall, the ability of the N-terminal of PfSRA antibodies to evoke inhibition of merozoite invasion of erythrocytes and conserved amino acid at low genetic diversity suggest that the N-terminal of PfSRA could be evaluated as a vaccine candidate against P. falciparum infection. Frontiers Media S.A. 2021-08-05 /pmc/articles/PMC8378275/ /pubmed/34421996 http://dx.doi.org/10.3389/fgene.2021.688606 Text en Copyright © 2021 Yang, Liu, Xu, Sun, Xu, Zhang, Tang, Zhu, Liu, Cao and Cheng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Yang, Bo
Liu, Hong
Xu, Qin-Wen
Sun, Yi-Fan
Xu, Sui
Zhang, Hao
Tang, Jian-Xia
Zhu, Guo-Ding
Liu, Yao-Bao
Cao, Jun
Cheng, Yang
Genetic Diversity Analysis of Surface-Related Antigen (SRA) in Plasmodium falciparum Imported From Africa to China
title Genetic Diversity Analysis of Surface-Related Antigen (SRA) in Plasmodium falciparum Imported From Africa to China
title_full Genetic Diversity Analysis of Surface-Related Antigen (SRA) in Plasmodium falciparum Imported From Africa to China
title_fullStr Genetic Diversity Analysis of Surface-Related Antigen (SRA) in Plasmodium falciparum Imported From Africa to China
title_full_unstemmed Genetic Diversity Analysis of Surface-Related Antigen (SRA) in Plasmodium falciparum Imported From Africa to China
title_short Genetic Diversity Analysis of Surface-Related Antigen (SRA) in Plasmodium falciparum Imported From Africa to China
title_sort genetic diversity analysis of surface-related antigen (sra) in plasmodium falciparum imported from africa to china
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8378275/
https://www.ncbi.nlm.nih.gov/pubmed/34421996
http://dx.doi.org/10.3389/fgene.2021.688606
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