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Evaluation of the ability of the trypsin-like peptidase activity assay to detect severe periodontitis

OBJECTIVES: N-benzoyl-DL-arginine peptidase (trypsin-like peptidase) is specifically produced by certain strains of periodontitis-associated bacteria. We aimed to examine the effectiveness of an objectively quantified trypsin-like peptidase activity assay (TLP-AA) for detecting severe periodontitis....

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Autores principales: Iwasaki, Masanori, Usui, Michihiko, Ariyoshi, Wataru, Nakashima, Keisuke, Nagai-Yoshioka, Yoshie, Inoue, Maki, Kobayashi, Kaoru, Nishihara, Tatsuji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8378714/
https://www.ncbi.nlm.nih.gov/pubmed/34415974
http://dx.doi.org/10.1371/journal.pone.0256538
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author Iwasaki, Masanori
Usui, Michihiko
Ariyoshi, Wataru
Nakashima, Keisuke
Nagai-Yoshioka, Yoshie
Inoue, Maki
Kobayashi, Kaoru
Nishihara, Tatsuji
author_facet Iwasaki, Masanori
Usui, Michihiko
Ariyoshi, Wataru
Nakashima, Keisuke
Nagai-Yoshioka, Yoshie
Inoue, Maki
Kobayashi, Kaoru
Nishihara, Tatsuji
author_sort Iwasaki, Masanori
collection PubMed
description OBJECTIVES: N-benzoyl-DL-arginine peptidase (trypsin-like peptidase) is specifically produced by certain strains of periodontitis-associated bacteria. We aimed to examine the effectiveness of an objectively quantified trypsin-like peptidase activity assay (TLP-AA) for detecting severe periodontitis. METHODS: The study population included 347 adults (108 men and 239 women; average age, 43.3 years) who underwent a full-mouth periodontal examination. Specimens for the TLP-AA were obtained using tongue swabs. Using a color reader, the TLP-AA results were obtained as a* values, with higher positive a* values indicating an increased intense enzymatic activity. The predictive validity of the TLP-AA results for severe periodontitis was assessed using receiver operating characteristic curve analysis and the periodontitis case definition provided by the Centers for Disease Control and Prevention/American Academy of Periodontology as the gold standard. Furthermore, multivariable logistic regression analyses were performed to predict severe periodontitis using the TLP-AA results and health characteristics, as the exposure variables. RESULTS: Severe periodontitis was observed in 5.2% of the participants. TLP-AA had high diagnostic accuracy for severe periodontitis, with an area under the curve of 0.83 (95% confidence interval [CI]: 0.75–0.92). The cut-off score for the a* value that best differentiated individuals with severe periodontitis was 0.09, with a sensitivity of 83% and specificity of 77%. Multivariable logistic regression analyses revealed that the TLP-AA results were significantly associated with severe periodontitis after adjusting for health characteristics (adjusted odds ratios: 1.90 [95% CI: 1.37–2.62] for the a* value). CONCLUSIONS: Objectively quantified TLP-AA results are potentially useful for detecting severe periodontitis in epidemiological surveillance.
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spelling pubmed-83787142021-08-21 Evaluation of the ability of the trypsin-like peptidase activity assay to detect severe periodontitis Iwasaki, Masanori Usui, Michihiko Ariyoshi, Wataru Nakashima, Keisuke Nagai-Yoshioka, Yoshie Inoue, Maki Kobayashi, Kaoru Nishihara, Tatsuji PLoS One Research Article OBJECTIVES: N-benzoyl-DL-arginine peptidase (trypsin-like peptidase) is specifically produced by certain strains of periodontitis-associated bacteria. We aimed to examine the effectiveness of an objectively quantified trypsin-like peptidase activity assay (TLP-AA) for detecting severe periodontitis. METHODS: The study population included 347 adults (108 men and 239 women; average age, 43.3 years) who underwent a full-mouth periodontal examination. Specimens for the TLP-AA were obtained using tongue swabs. Using a color reader, the TLP-AA results were obtained as a* values, with higher positive a* values indicating an increased intense enzymatic activity. The predictive validity of the TLP-AA results for severe periodontitis was assessed using receiver operating characteristic curve analysis and the periodontitis case definition provided by the Centers for Disease Control and Prevention/American Academy of Periodontology as the gold standard. Furthermore, multivariable logistic regression analyses were performed to predict severe periodontitis using the TLP-AA results and health characteristics, as the exposure variables. RESULTS: Severe periodontitis was observed in 5.2% of the participants. TLP-AA had high diagnostic accuracy for severe periodontitis, with an area under the curve of 0.83 (95% confidence interval [CI]: 0.75–0.92). The cut-off score for the a* value that best differentiated individuals with severe periodontitis was 0.09, with a sensitivity of 83% and specificity of 77%. Multivariable logistic regression analyses revealed that the TLP-AA results were significantly associated with severe periodontitis after adjusting for health characteristics (adjusted odds ratios: 1.90 [95% CI: 1.37–2.62] for the a* value). CONCLUSIONS: Objectively quantified TLP-AA results are potentially useful for detecting severe periodontitis in epidemiological surveillance. Public Library of Science 2021-08-20 /pmc/articles/PMC8378714/ /pubmed/34415974 http://dx.doi.org/10.1371/journal.pone.0256538 Text en © 2021 Iwasaki et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Iwasaki, Masanori
Usui, Michihiko
Ariyoshi, Wataru
Nakashima, Keisuke
Nagai-Yoshioka, Yoshie
Inoue, Maki
Kobayashi, Kaoru
Nishihara, Tatsuji
Evaluation of the ability of the trypsin-like peptidase activity assay to detect severe periodontitis
title Evaluation of the ability of the trypsin-like peptidase activity assay to detect severe periodontitis
title_full Evaluation of the ability of the trypsin-like peptidase activity assay to detect severe periodontitis
title_fullStr Evaluation of the ability of the trypsin-like peptidase activity assay to detect severe periodontitis
title_full_unstemmed Evaluation of the ability of the trypsin-like peptidase activity assay to detect severe periodontitis
title_short Evaluation of the ability of the trypsin-like peptidase activity assay to detect severe periodontitis
title_sort evaluation of the ability of the trypsin-like peptidase activity assay to detect severe periodontitis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8378714/
https://www.ncbi.nlm.nih.gov/pubmed/34415974
http://dx.doi.org/10.1371/journal.pone.0256538
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