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In vitro investigation of the impact of pulsatile blood flow on the vascular architecture of decellularized porcine kidneys
A method was established using a scaffold-bioreactor system to examine the impact pulsatile blood flow has on the decellularized porcine kidney vascular architecture and functionality. These scaffolds were subjected to continuous arterial perfusion of whole blood at normal physiological (650 ml/min ...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379263/ https://www.ncbi.nlm.nih.gov/pubmed/34417499 http://dx.doi.org/10.1038/s41598-021-95924-5 |
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author | Corridon, Peter R. |
author_facet | Corridon, Peter R. |
author_sort | Corridon, Peter R. |
collection | PubMed |
description | A method was established using a scaffold-bioreactor system to examine the impact pulsatile blood flow has on the decellularized porcine kidney vascular architecture and functionality. These scaffolds were subjected to continuous arterial perfusion of whole blood at normal physiological (650 ml/min and 500 ml/min) and pathophysiological (200 ml/min) rates to examine dynamic changes in venous outflow and micro-/macrovascular structure and patency. Scaffolds subjected to normal arterial perfusion rates observed drops in venous outflow over 24 h. These reductions rose from roughly 40% after 12 h to 60% after 24 h. There were no apparent signs of clotting at the renal artery, renal vein, and ureter. In comparison, venous flow rates decreased by 80% to 100% across the 24 h in acellular scaffolds hypoperfused at a rate of 200 ml/min. These kidneys also appeared intact on the surface after perfusion. However, they presented several arterial, venous, and ureteral clots. Fluoroscopic angiography confirmed substantial alterations to normal arterial branching patterns and patency, as well as parenchymal damage. Scanning electron microscopy revealed that pulsatile blood perfusion significantly disrupted glomerular microarchitecture. This study provides new insight into circumstances that limit scaffold viability and a simplified model to analyze conditions needed to prepare more durable scaffolds for long-term transplantation. |
format | Online Article Text |
id | pubmed-8379263 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-83792632021-09-01 In vitro investigation of the impact of pulsatile blood flow on the vascular architecture of decellularized porcine kidneys Corridon, Peter R. Sci Rep Article A method was established using a scaffold-bioreactor system to examine the impact pulsatile blood flow has on the decellularized porcine kidney vascular architecture and functionality. These scaffolds were subjected to continuous arterial perfusion of whole blood at normal physiological (650 ml/min and 500 ml/min) and pathophysiological (200 ml/min) rates to examine dynamic changes in venous outflow and micro-/macrovascular structure and patency. Scaffolds subjected to normal arterial perfusion rates observed drops in venous outflow over 24 h. These reductions rose from roughly 40% after 12 h to 60% after 24 h. There were no apparent signs of clotting at the renal artery, renal vein, and ureter. In comparison, venous flow rates decreased by 80% to 100% across the 24 h in acellular scaffolds hypoperfused at a rate of 200 ml/min. These kidneys also appeared intact on the surface after perfusion. However, they presented several arterial, venous, and ureteral clots. Fluoroscopic angiography confirmed substantial alterations to normal arterial branching patterns and patency, as well as parenchymal damage. Scanning electron microscopy revealed that pulsatile blood perfusion significantly disrupted glomerular microarchitecture. This study provides new insight into circumstances that limit scaffold viability and a simplified model to analyze conditions needed to prepare more durable scaffolds for long-term transplantation. Nature Publishing Group UK 2021-08-20 /pmc/articles/PMC8379263/ /pubmed/34417499 http://dx.doi.org/10.1038/s41598-021-95924-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Corridon, Peter R. In vitro investigation of the impact of pulsatile blood flow on the vascular architecture of decellularized porcine kidneys |
title | In vitro investigation of the impact of pulsatile blood flow on the vascular architecture of decellularized porcine kidneys |
title_full | In vitro investigation of the impact of pulsatile blood flow on the vascular architecture of decellularized porcine kidneys |
title_fullStr | In vitro investigation of the impact of pulsatile blood flow on the vascular architecture of decellularized porcine kidneys |
title_full_unstemmed | In vitro investigation of the impact of pulsatile blood flow on the vascular architecture of decellularized porcine kidneys |
title_short | In vitro investigation of the impact of pulsatile blood flow on the vascular architecture of decellularized porcine kidneys |
title_sort | in vitro investigation of the impact of pulsatile blood flow on the vascular architecture of decellularized porcine kidneys |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379263/ https://www.ncbi.nlm.nih.gov/pubmed/34417499 http://dx.doi.org/10.1038/s41598-021-95924-5 |
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