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Dose response of biochar and wood vinegar on in vitro batch culture ruminal fermentation using contrasting feed substrates
Within Australia, approximately 6.4% of total greenhouse gas emissions are from animal methane (CH(4)) derived from enteric fermentation. Mitigation of ruminant CH(4) is a key concept in support of sustainable agriculture production; dietary manipulations a viable strategy to lower CH(4) release dur...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379518/ https://www.ncbi.nlm.nih.gov/pubmed/34430799 http://dx.doi.org/10.1093/tas/txab107 |
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author | O’Reilly, Grace Celia Huo, Yuxin Meale, Sarah Jade Chaves, Alex V |
author_facet | O’Reilly, Grace Celia Huo, Yuxin Meale, Sarah Jade Chaves, Alex V |
author_sort | O’Reilly, Grace Celia |
collection | PubMed |
description | Within Australia, approximately 6.4% of total greenhouse gas emissions are from animal methane (CH(4)) derived from enteric fermentation. Mitigation of ruminant CH(4) is a key concept in support of sustainable agriculture production; dietary manipulations a viable strategy to lower CH(4) release during enteric fermentation. In order to determine the effects of dose response of biochar and wood vinegar supplementation on fermentation parameters and CH(4) production, this study utilized in vitro batch culture incubations. It is hypothesized that the addition of either biochar or wood vinegar will successfully reduce enteric CH(4) emissions without negative modification of other fermentation parameters. Three feed substrates (vegetable mixed ration, maize silage, and winter pasture) were separated into treatments containing either biochar at 0%, 0.5%, 1%, 2%, and 4% DM replacing substrate (w/w basis), or wood vinegar at 0%, 0.25%, 0.5%, 1%, and 2% into incubation media volume (v/v). At 6, 12, and 24 hours after inoculation, total gas volume, and methane (CH(4) %) were measured. Volatile fatty acid (VFA) concentrations, media pH, and in vitro dry matter digestibility were measured at 24 hours. Biochar at various dosages had no effect (P > 0.05) on fermentation characteristics other than decreased in vitro dry matter digestibility (IVDMD; P = 0.01) at 2% and 4% (DM basis) inclusion. Similar to biochar, dose response of wood vinegar had no effect on in vitro fermentation characteristics. However, feed substrate had major effects on all fermentation parameters (P = 0.01) where winter pasture > vegetable mixed ration > maize silage for all recorded fermentation characteristics. Biochar and wood vinegar supplementation were ineffectual in mitigating CH(4) production or modifying fermentation characteristics, thus rejecting the initial hypothesis. These results suggest the use of biochar is not an effective tool for methane mitigation in ruminant livestock and infers that studies previously reporting success must better define the systemic mechanisms responsible for the reduction in CH(4). |
format | Online Article Text |
id | pubmed-8379518 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-83795182021-08-23 Dose response of biochar and wood vinegar on in vitro batch culture ruminal fermentation using contrasting feed substrates O’Reilly, Grace Celia Huo, Yuxin Meale, Sarah Jade Chaves, Alex V Transl Anim Sci Ruminant Nutrition Within Australia, approximately 6.4% of total greenhouse gas emissions are from animal methane (CH(4)) derived from enteric fermentation. Mitigation of ruminant CH(4) is a key concept in support of sustainable agriculture production; dietary manipulations a viable strategy to lower CH(4) release during enteric fermentation. In order to determine the effects of dose response of biochar and wood vinegar supplementation on fermentation parameters and CH(4) production, this study utilized in vitro batch culture incubations. It is hypothesized that the addition of either biochar or wood vinegar will successfully reduce enteric CH(4) emissions without negative modification of other fermentation parameters. Three feed substrates (vegetable mixed ration, maize silage, and winter pasture) were separated into treatments containing either biochar at 0%, 0.5%, 1%, 2%, and 4% DM replacing substrate (w/w basis), or wood vinegar at 0%, 0.25%, 0.5%, 1%, and 2% into incubation media volume (v/v). At 6, 12, and 24 hours after inoculation, total gas volume, and methane (CH(4) %) were measured. Volatile fatty acid (VFA) concentrations, media pH, and in vitro dry matter digestibility were measured at 24 hours. Biochar at various dosages had no effect (P > 0.05) on fermentation characteristics other than decreased in vitro dry matter digestibility (IVDMD; P = 0.01) at 2% and 4% (DM basis) inclusion. Similar to biochar, dose response of wood vinegar had no effect on in vitro fermentation characteristics. However, feed substrate had major effects on all fermentation parameters (P = 0.01) where winter pasture > vegetable mixed ration > maize silage for all recorded fermentation characteristics. Biochar and wood vinegar supplementation were ineffectual in mitigating CH(4) production or modifying fermentation characteristics, thus rejecting the initial hypothesis. These results suggest the use of biochar is not an effective tool for methane mitigation in ruminant livestock and infers that studies previously reporting success must better define the systemic mechanisms responsible for the reduction in CH(4). Oxford University Press 2021-06-12 /pmc/articles/PMC8379518/ /pubmed/34430799 http://dx.doi.org/10.1093/tas/txab107 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of the American Society of Animal Science. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Ruminant Nutrition O’Reilly, Grace Celia Huo, Yuxin Meale, Sarah Jade Chaves, Alex V Dose response of biochar and wood vinegar on in vitro batch culture ruminal fermentation using contrasting feed substrates |
title | Dose response of biochar and wood vinegar on in vitro batch culture ruminal fermentation using contrasting feed substrates |
title_full | Dose response of biochar and wood vinegar on in vitro batch culture ruminal fermentation using contrasting feed substrates |
title_fullStr | Dose response of biochar and wood vinegar on in vitro batch culture ruminal fermentation using contrasting feed substrates |
title_full_unstemmed | Dose response of biochar and wood vinegar on in vitro batch culture ruminal fermentation using contrasting feed substrates |
title_short | Dose response of biochar and wood vinegar on in vitro batch culture ruminal fermentation using contrasting feed substrates |
title_sort | dose response of biochar and wood vinegar on in vitro batch culture ruminal fermentation using contrasting feed substrates |
topic | Ruminant Nutrition |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379518/ https://www.ncbi.nlm.nih.gov/pubmed/34430799 http://dx.doi.org/10.1093/tas/txab107 |
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