Cargando…

Dose response of biochar and wood vinegar on in vitro batch culture ruminal fermentation using contrasting feed substrates

Within Australia, approximately 6.4% of total greenhouse gas emissions are from animal methane (CH(4)) derived from enteric fermentation. Mitigation of ruminant CH(4) is a key concept in support of sustainable agriculture production; dietary manipulations a viable strategy to lower CH(4) release dur...

Descripción completa

Detalles Bibliográficos
Autores principales: O’Reilly, Grace Celia, Huo, Yuxin, Meale, Sarah Jade, Chaves, Alex V
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379518/
https://www.ncbi.nlm.nih.gov/pubmed/34430799
http://dx.doi.org/10.1093/tas/txab107
_version_ 1783741025952464896
author O’Reilly, Grace Celia
Huo, Yuxin
Meale, Sarah Jade
Chaves, Alex V
author_facet O’Reilly, Grace Celia
Huo, Yuxin
Meale, Sarah Jade
Chaves, Alex V
author_sort O’Reilly, Grace Celia
collection PubMed
description Within Australia, approximately 6.4% of total greenhouse gas emissions are from animal methane (CH(4)) derived from enteric fermentation. Mitigation of ruminant CH(4) is a key concept in support of sustainable agriculture production; dietary manipulations a viable strategy to lower CH(4) release during enteric fermentation. In order to determine the effects of dose response of biochar and wood vinegar supplementation on fermentation parameters and CH(4) production, this study utilized in vitro batch culture incubations. It is hypothesized that the addition of either biochar or wood vinegar will successfully reduce enteric CH(4) emissions without negative modification of other fermentation parameters. Three feed substrates (vegetable mixed ration, maize silage, and winter pasture) were separated into treatments containing either biochar at 0%, 0.5%, 1%, 2%, and 4% DM replacing substrate (w/w basis), or wood vinegar at 0%, 0.25%, 0.5%, 1%, and 2% into incubation media volume (v/v). At 6, 12, and 24 hours after inoculation, total gas volume, and methane (CH(4) %) were measured. Volatile fatty acid (VFA) concentrations, media pH, and in vitro dry matter digestibility were measured at 24 hours. Biochar at various dosages had no effect (P > 0.05) on fermentation characteristics other than decreased in vitro dry matter digestibility (IVDMD; P = 0.01) at 2% and 4% (DM basis) inclusion. Similar to biochar, dose response of wood vinegar had no effect on in vitro fermentation characteristics. However, feed substrate had major effects on all fermentation parameters (P = 0.01) where winter pasture > vegetable mixed ration > maize silage for all recorded fermentation characteristics. Biochar and wood vinegar supplementation were ineffectual in mitigating CH(4) production or modifying fermentation characteristics, thus rejecting the initial hypothesis. These results suggest the use of biochar is not an effective tool for methane mitigation in ruminant livestock and infers that studies previously reporting success must better define the systemic mechanisms responsible for the reduction in CH(4).
format Online
Article
Text
id pubmed-8379518
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-83795182021-08-23 Dose response of biochar and wood vinegar on in vitro batch culture ruminal fermentation using contrasting feed substrates O’Reilly, Grace Celia Huo, Yuxin Meale, Sarah Jade Chaves, Alex V Transl Anim Sci Ruminant Nutrition Within Australia, approximately 6.4% of total greenhouse gas emissions are from animal methane (CH(4)) derived from enteric fermentation. Mitigation of ruminant CH(4) is a key concept in support of sustainable agriculture production; dietary manipulations a viable strategy to lower CH(4) release during enteric fermentation. In order to determine the effects of dose response of biochar and wood vinegar supplementation on fermentation parameters and CH(4) production, this study utilized in vitro batch culture incubations. It is hypothesized that the addition of either biochar or wood vinegar will successfully reduce enteric CH(4) emissions without negative modification of other fermentation parameters. Three feed substrates (vegetable mixed ration, maize silage, and winter pasture) were separated into treatments containing either biochar at 0%, 0.5%, 1%, 2%, and 4% DM replacing substrate (w/w basis), or wood vinegar at 0%, 0.25%, 0.5%, 1%, and 2% into incubation media volume (v/v). At 6, 12, and 24 hours after inoculation, total gas volume, and methane (CH(4) %) were measured. Volatile fatty acid (VFA) concentrations, media pH, and in vitro dry matter digestibility were measured at 24 hours. Biochar at various dosages had no effect (P > 0.05) on fermentation characteristics other than decreased in vitro dry matter digestibility (IVDMD; P = 0.01) at 2% and 4% (DM basis) inclusion. Similar to biochar, dose response of wood vinegar had no effect on in vitro fermentation characteristics. However, feed substrate had major effects on all fermentation parameters (P = 0.01) where winter pasture > vegetable mixed ration > maize silage for all recorded fermentation characteristics. Biochar and wood vinegar supplementation were ineffectual in mitigating CH(4) production or modifying fermentation characteristics, thus rejecting the initial hypothesis. These results suggest the use of biochar is not an effective tool for methane mitigation in ruminant livestock and infers that studies previously reporting success must better define the systemic mechanisms responsible for the reduction in CH(4). Oxford University Press 2021-06-12 /pmc/articles/PMC8379518/ /pubmed/34430799 http://dx.doi.org/10.1093/tas/txab107 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of the American Society of Animal Science. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Ruminant Nutrition
O’Reilly, Grace Celia
Huo, Yuxin
Meale, Sarah Jade
Chaves, Alex V
Dose response of biochar and wood vinegar on in vitro batch culture ruminal fermentation using contrasting feed substrates
title Dose response of biochar and wood vinegar on in vitro batch culture ruminal fermentation using contrasting feed substrates
title_full Dose response of biochar and wood vinegar on in vitro batch culture ruminal fermentation using contrasting feed substrates
title_fullStr Dose response of biochar and wood vinegar on in vitro batch culture ruminal fermentation using contrasting feed substrates
title_full_unstemmed Dose response of biochar and wood vinegar on in vitro batch culture ruminal fermentation using contrasting feed substrates
title_short Dose response of biochar and wood vinegar on in vitro batch culture ruminal fermentation using contrasting feed substrates
title_sort dose response of biochar and wood vinegar on in vitro batch culture ruminal fermentation using contrasting feed substrates
topic Ruminant Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379518/
https://www.ncbi.nlm.nih.gov/pubmed/34430799
http://dx.doi.org/10.1093/tas/txab107
work_keys_str_mv AT oreillygracecelia doseresponseofbiocharandwoodvinegaroninvitrobatchcultureruminalfermentationusingcontrastingfeedsubstrates
AT huoyuxin doseresponseofbiocharandwoodvinegaroninvitrobatchcultureruminalfermentationusingcontrastingfeedsubstrates
AT mealesarahjade doseresponseofbiocharandwoodvinegaroninvitrobatchcultureruminalfermentationusingcontrastingfeedsubstrates
AT chavesalexv doseresponseofbiocharandwoodvinegaroninvitrobatchcultureruminalfermentationusingcontrastingfeedsubstrates