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Analysis of local protein accumulation kinetics by live-cell imaging in yeast systems

Microscopy-based analysis of protein accumulation at a given subcellular location in real time provides invaluable insights into the function of a protein in a specific process. Here, we describe a detailed protocol for determining protein accumulation kinetics at the division site in the budding ye...

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Detalles Bibliográficos
Autores principales: Okada, Hiroki, MacTaggart, Brittany, Bi, Erfei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379524/
https://www.ncbi.nlm.nih.gov/pubmed/34458867
http://dx.doi.org/10.1016/j.xpro.2021.100733

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