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Analysis of local protein accumulation kinetics by live-cell imaging in yeast systems
Microscopy-based analysis of protein accumulation at a given subcellular location in real time provides invaluable insights into the function of a protein in a specific process. Here, we describe a detailed protocol for determining protein accumulation kinetics at the division site in the budding ye...
Autores principales: | Okada, Hiroki, MacTaggart, Brittany, Bi, Erfei |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379524/ https://www.ncbi.nlm.nih.gov/pubmed/34458867 http://dx.doi.org/10.1016/j.xpro.2021.100733 |
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