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Toxic potential of botulinum toxin type A on senescence in a Drosophila melanogaster model
Botulinum toxin type-A (BoNT/A) application, especially neurological disorders, has been spread nowadays while it may cause side effects. The current study aimed to assess the BoNT/A dose-dependent effect on induction of aging in the Drosophila melanogaster model. The third instar larvae of Drosophi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379625/ https://www.ncbi.nlm.nih.gov/pubmed/34458104 http://dx.doi.org/10.1016/j.toxrep.2021.08.002 |
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author | Fooladvand, Farnoosh Tahouri, Vida Baeeri, Maryam Minaei, Tayebeh Rahimifard, Mahban Hodjat, Mahshid Khorasani, Reza Haghi-Aminjan, Hamed Abdollahi, Mohammad |
author_facet | Fooladvand, Farnoosh Tahouri, Vida Baeeri, Maryam Minaei, Tayebeh Rahimifard, Mahban Hodjat, Mahshid Khorasani, Reza Haghi-Aminjan, Hamed Abdollahi, Mohammad |
author_sort | Fooladvand, Farnoosh |
collection | PubMed |
description | Botulinum toxin type-A (BoNT/A) application, especially neurological disorders, has been spread nowadays while it may cause side effects. The current study aimed to assess the BoNT/A dose-dependent effect on induction of aging in the Drosophila melanogaster model. The third instar larvae of Drosophila melanogaster were exposed to ¼ LC(50), ½ LC(50), and LC(50) of BoNT/A in the Drosophila diet for 48 h while H(2)O(2) 1% was used as a positive control. After the exposure time, some larvae were collected for molecular study, including gene expression analysis, comet assay, oxidative stress markers, and the phenotype changes. BoNT/A induced dose-dependent cytotoxicity, elevated reactive oxygen species (ROS) levels, and superoxide dismutase (SOD) enzyme activity. In addition, it caused DNA damage and activated caspase-3 and -9, and reduced the body size of the fly, especially in high doses. In line with the purpose of the study, aging markers, including β-galactosidase (β-gal), p16, p21, p38, and p53, were up-regulated by BoNT/A low dose. BoNT/A activates the aging pathway in the low dose, and increasing the dose induces toxicity, including oxidative stress, DNA damage, and apoptosis. |
format | Online Article Text |
id | pubmed-8379625 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-83796252021-08-27 Toxic potential of botulinum toxin type A on senescence in a Drosophila melanogaster model Fooladvand, Farnoosh Tahouri, Vida Baeeri, Maryam Minaei, Tayebeh Rahimifard, Mahban Hodjat, Mahshid Khorasani, Reza Haghi-Aminjan, Hamed Abdollahi, Mohammad Toxicol Rep Acute to chronic adverse effects caused by herbal products and natural bioactive compound Botulinum toxin type-A (BoNT/A) application, especially neurological disorders, has been spread nowadays while it may cause side effects. The current study aimed to assess the BoNT/A dose-dependent effect on induction of aging in the Drosophila melanogaster model. The third instar larvae of Drosophila melanogaster were exposed to ¼ LC(50), ½ LC(50), and LC(50) of BoNT/A in the Drosophila diet for 48 h while H(2)O(2) 1% was used as a positive control. After the exposure time, some larvae were collected for molecular study, including gene expression analysis, comet assay, oxidative stress markers, and the phenotype changes. BoNT/A induced dose-dependent cytotoxicity, elevated reactive oxygen species (ROS) levels, and superoxide dismutase (SOD) enzyme activity. In addition, it caused DNA damage and activated caspase-3 and -9, and reduced the body size of the fly, especially in high doses. In line with the purpose of the study, aging markers, including β-galactosidase (β-gal), p16, p21, p38, and p53, were up-regulated by BoNT/A low dose. BoNT/A activates the aging pathway in the low dose, and increasing the dose induces toxicity, including oxidative stress, DNA damage, and apoptosis. Elsevier 2021-08-16 /pmc/articles/PMC8379625/ /pubmed/34458104 http://dx.doi.org/10.1016/j.toxrep.2021.08.002 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Acute to chronic adverse effects caused by herbal products and natural bioactive compound Fooladvand, Farnoosh Tahouri, Vida Baeeri, Maryam Minaei, Tayebeh Rahimifard, Mahban Hodjat, Mahshid Khorasani, Reza Haghi-Aminjan, Hamed Abdollahi, Mohammad Toxic potential of botulinum toxin type A on senescence in a Drosophila melanogaster model |
title | Toxic potential of botulinum toxin type A on senescence in a Drosophila melanogaster model |
title_full | Toxic potential of botulinum toxin type A on senescence in a Drosophila melanogaster model |
title_fullStr | Toxic potential of botulinum toxin type A on senescence in a Drosophila melanogaster model |
title_full_unstemmed | Toxic potential of botulinum toxin type A on senescence in a Drosophila melanogaster model |
title_short | Toxic potential of botulinum toxin type A on senescence in a Drosophila melanogaster model |
title_sort | toxic potential of botulinum toxin type a on senescence in a drosophila melanogaster model |
topic | Acute to chronic adverse effects caused by herbal products and natural bioactive compound |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379625/ https://www.ncbi.nlm.nih.gov/pubmed/34458104 http://dx.doi.org/10.1016/j.toxrep.2021.08.002 |
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