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Region-specific effects of ultrasound on individual neurons in the awake mammalian brain

Ultrasound modulates brain activity. However, it remains unclear how ultrasound affects individual neurons in the brain, where neural circuit architecture is intact and different brain regions exhibit distinct tissue properties. Using a high-resolution calcium imaging technique, we characterized the...

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Autores principales: Tseng, Hua-an, Sherman, Jack, Bortz, Emma, Mohammed, Ali, Gritton, Howard J., Bensussen, Seth, Tang, Rockwell P., Zemel, Dana, Szabo, Thomas, Han, Xue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379692/
https://www.ncbi.nlm.nih.gov/pubmed/34458703
http://dx.doi.org/10.1016/j.isci.2021.102955
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author Tseng, Hua-an
Sherman, Jack
Bortz, Emma
Mohammed, Ali
Gritton, Howard J.
Bensussen, Seth
Tang, Rockwell P.
Zemel, Dana
Szabo, Thomas
Han, Xue
author_facet Tseng, Hua-an
Sherman, Jack
Bortz, Emma
Mohammed, Ali
Gritton, Howard J.
Bensussen, Seth
Tang, Rockwell P.
Zemel, Dana
Szabo, Thomas
Han, Xue
author_sort Tseng, Hua-an
collection PubMed
description Ultrasound modulates brain activity. However, it remains unclear how ultrasound affects individual neurons in the brain, where neural circuit architecture is intact and different brain regions exhibit distinct tissue properties. Using a high-resolution calcium imaging technique, we characterized the effect of ultrasound stimulation on thousands of individual neurons in the hippocampus and the motor cortex of awake mice. We found that brief 100-ms-long ultrasound pulses increase intracellular calcium in a large fraction of individual neurons in both brain regions. Ultrasound-evoked calcium response in hippocampal neurons exhibits a rapid onset with a latency shorter than 50 ms. The evoked response in the hippocampus is shorter in duration and smaller in magnitude than that in the motor cortex. These results demonstrate that noninvasive ultrasound stimulation transiently increases intracellular calcium in individual neurons in awake mice, and the evoked response profiles are brain region specific.
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spelling pubmed-83796922021-08-27 Region-specific effects of ultrasound on individual neurons in the awake mammalian brain Tseng, Hua-an Sherman, Jack Bortz, Emma Mohammed, Ali Gritton, Howard J. Bensussen, Seth Tang, Rockwell P. Zemel, Dana Szabo, Thomas Han, Xue iScience Article Ultrasound modulates brain activity. However, it remains unclear how ultrasound affects individual neurons in the brain, where neural circuit architecture is intact and different brain regions exhibit distinct tissue properties. Using a high-resolution calcium imaging technique, we characterized the effect of ultrasound stimulation on thousands of individual neurons in the hippocampus and the motor cortex of awake mice. We found that brief 100-ms-long ultrasound pulses increase intracellular calcium in a large fraction of individual neurons in both brain regions. Ultrasound-evoked calcium response in hippocampal neurons exhibits a rapid onset with a latency shorter than 50 ms. The evoked response in the hippocampus is shorter in duration and smaller in magnitude than that in the motor cortex. These results demonstrate that noninvasive ultrasound stimulation transiently increases intracellular calcium in individual neurons in awake mice, and the evoked response profiles are brain region specific. Elsevier 2021-08-08 /pmc/articles/PMC8379692/ /pubmed/34458703 http://dx.doi.org/10.1016/j.isci.2021.102955 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Tseng, Hua-an
Sherman, Jack
Bortz, Emma
Mohammed, Ali
Gritton, Howard J.
Bensussen, Seth
Tang, Rockwell P.
Zemel, Dana
Szabo, Thomas
Han, Xue
Region-specific effects of ultrasound on individual neurons in the awake mammalian brain
title Region-specific effects of ultrasound on individual neurons in the awake mammalian brain
title_full Region-specific effects of ultrasound on individual neurons in the awake mammalian brain
title_fullStr Region-specific effects of ultrasound on individual neurons in the awake mammalian brain
title_full_unstemmed Region-specific effects of ultrasound on individual neurons in the awake mammalian brain
title_short Region-specific effects of ultrasound on individual neurons in the awake mammalian brain
title_sort region-specific effects of ultrasound on individual neurons in the awake mammalian brain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379692/
https://www.ncbi.nlm.nih.gov/pubmed/34458703
http://dx.doi.org/10.1016/j.isci.2021.102955
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