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NEK7 promotes gastric cancer progression as a cell proliferation regulator

BACKGROUND: Gastric cancer is one of the most common malignant tumors of the digestive system. However, its targeted therapy develops at a slow pace. Thus, exploring the mechanisms of the malignant behavior of gastric cancer cells is crucial to exploit its treatment. Mammalian never-in-mitosis A (NI...

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Autores principales: Li, Yi-Ke, Zhu, Xiao-Ran, Zhan, Yue, Yuan, Wen-Zhen, Jin, Wei-Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379724/
https://www.ncbi.nlm.nih.gov/pubmed/34419048
http://dx.doi.org/10.1186/s12935-021-02148-8
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author Li, Yi-Ke
Zhu, Xiao-Ran
Zhan, Yue
Yuan, Wen-Zhen
Jin, Wei-Lin
author_facet Li, Yi-Ke
Zhu, Xiao-Ran
Zhan, Yue
Yuan, Wen-Zhen
Jin, Wei-Lin
author_sort Li, Yi-Ke
collection PubMed
description BACKGROUND: Gastric cancer is one of the most common malignant tumors of the digestive system. However, its targeted therapy develops at a slow pace. Thus, exploring the mechanisms of the malignant behavior of gastric cancer cells is crucial to exploit its treatment. Mammalian never-in-mitosis A (NIMA)-related kinases (NEKs) are considered to play a significant role in cancer cell proliferation. However, no study has reported on NIMA family proteins in gastric cancer. METHODS: Bioinformatics analysis was employed to clarify the expression patterns of NEK1–NEK11 and their effects on prognosis. The effects of NEK7 on immune infiltration and NEK7 related pathways were also analyzed. At the cell level, 5-ethynyl-2-deoxyuridine, cell cycle, and Cell Counting Kit-8 assays were utilized to clarify the effect of NEK7 on gastric cancer cell proliferation. A mouse subcutaneous model revealed the regulating effect of NEK7 on gastric cancer cell proliferation in vivo. RESULTS: Bioinformatics analysis revealed that NEK7 is upregulated in gastric cancer and is related to poor prognosis. NEK7 is also related to T-stage, which is closely associated with cell proliferation. Further analysis showed that NEK7 was correlated with infiltration of multiple immune cells as well as gastric cancer-related pathways. Cell experiments indicated the promoting effect of NEK7 on cell proliferation, while the absence of NEK7 could lead to inhibition of gastric cancer proliferation and G1/S arrest. CONCLUSION: NEK7 exerts a regulatory effect on cell proliferation and is closely related to tumor immune infiltration. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12935-021-02148-8.
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spelling pubmed-83797242021-08-23 NEK7 promotes gastric cancer progression as a cell proliferation regulator Li, Yi-Ke Zhu, Xiao-Ran Zhan, Yue Yuan, Wen-Zhen Jin, Wei-Lin Cancer Cell Int Primary Research BACKGROUND: Gastric cancer is one of the most common malignant tumors of the digestive system. However, its targeted therapy develops at a slow pace. Thus, exploring the mechanisms of the malignant behavior of gastric cancer cells is crucial to exploit its treatment. Mammalian never-in-mitosis A (NIMA)-related kinases (NEKs) are considered to play a significant role in cancer cell proliferation. However, no study has reported on NIMA family proteins in gastric cancer. METHODS: Bioinformatics analysis was employed to clarify the expression patterns of NEK1–NEK11 and their effects on prognosis. The effects of NEK7 on immune infiltration and NEK7 related pathways were also analyzed. At the cell level, 5-ethynyl-2-deoxyuridine, cell cycle, and Cell Counting Kit-8 assays were utilized to clarify the effect of NEK7 on gastric cancer cell proliferation. A mouse subcutaneous model revealed the regulating effect of NEK7 on gastric cancer cell proliferation in vivo. RESULTS: Bioinformatics analysis revealed that NEK7 is upregulated in gastric cancer and is related to poor prognosis. NEK7 is also related to T-stage, which is closely associated with cell proliferation. Further analysis showed that NEK7 was correlated with infiltration of multiple immune cells as well as gastric cancer-related pathways. Cell experiments indicated the promoting effect of NEK7 on cell proliferation, while the absence of NEK7 could lead to inhibition of gastric cancer proliferation and G1/S arrest. CONCLUSION: NEK7 exerts a regulatory effect on cell proliferation and is closely related to tumor immune infiltration. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12935-021-02148-8. BioMed Central 2021-08-21 /pmc/articles/PMC8379724/ /pubmed/34419048 http://dx.doi.org/10.1186/s12935-021-02148-8 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Primary Research
Li, Yi-Ke
Zhu, Xiao-Ran
Zhan, Yue
Yuan, Wen-Zhen
Jin, Wei-Lin
NEK7 promotes gastric cancer progression as a cell proliferation regulator
title NEK7 promotes gastric cancer progression as a cell proliferation regulator
title_full NEK7 promotes gastric cancer progression as a cell proliferation regulator
title_fullStr NEK7 promotes gastric cancer progression as a cell proliferation regulator
title_full_unstemmed NEK7 promotes gastric cancer progression as a cell proliferation regulator
title_short NEK7 promotes gastric cancer progression as a cell proliferation regulator
title_sort nek7 promotes gastric cancer progression as a cell proliferation regulator
topic Primary Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379724/
https://www.ncbi.nlm.nih.gov/pubmed/34419048
http://dx.doi.org/10.1186/s12935-021-02148-8
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