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Resistance and virulence distribution in enterococci isolated from broilers reared in two farming systems

BACKGROUND: The impact of enterococci in human health has been growing for the last decades, mainly due to their resistance to several antimicrobial agents. Human consumption of contaminated meat, especially poultry, has been identified as a possible route of transmission. The aim of the present stu...

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Autores principales: Semedo-Lemsaddek, Teresa, Bettencourt Cota, João, Ribeiro, Tânia, Pimentel, Amélia, Tavares, Luís, Bernando, Fernando, Oliveira, Manuela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379790/
https://www.ncbi.nlm.nih.gov/pubmed/34416919
http://dx.doi.org/10.1186/s13620-021-00201-6
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author Semedo-Lemsaddek, Teresa
Bettencourt Cota, João
Ribeiro, Tânia
Pimentel, Amélia
Tavares, Luís
Bernando, Fernando
Oliveira, Manuela
author_facet Semedo-Lemsaddek, Teresa
Bettencourt Cota, João
Ribeiro, Tânia
Pimentel, Amélia
Tavares, Luís
Bernando, Fernando
Oliveira, Manuela
author_sort Semedo-Lemsaddek, Teresa
collection PubMed
description BACKGROUND: The impact of enterococci in human health has been growing for the last decades, mainly due to their resistance to several antimicrobial agents. Human consumption of contaminated meat, especially poultry, has been identified as a possible route of transmission. The aim of the present study was to evaluate and compare the antimicrobial resistance profiles and virulence genes of enterococci isolated from Portuguese conventional and free-range broiler farms. RESULTS: Antibiotic susceptibility testing showed high frequencies of resistance to tetracycline in both farming systems. Resistance to erythromycin and gentamicin were detected in about half of the isolates. Resistance to penicillin was the less frequently observed and no vancomycin resistant isolates were identified. The majority of the enterococcal isolates, from either farming systems, were resistant to more than one antibiotic, and no statistical associations were found, except for penicillin resistance which associated with the genetic clusters. No differences were found between farming systems regarding the prevalence of tet(M), erm(B), aac (6′)-Ie-aph (2″)-Ia and pbp5 genes, nevertheless pbp5 prevalence was associated with the different genetic clusters. Hemolytic activity was identified in 26.47% of all isolates and gelatinase activity in 50%. The gelE gene was identified in the majority of the isolates, whereas esp and agg genes were rarely detected. The cylA determinant was not detected in any of the isolates. CONCLUSIONS: Overall, results suggest that similar resistance patterns and virulence genes can be found in both farming systems, though enterococci in free-range conditions should be less prone to acquire further resistance genes.
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spelling pubmed-83797902021-08-23 Resistance and virulence distribution in enterococci isolated from broilers reared in two farming systems Semedo-Lemsaddek, Teresa Bettencourt Cota, João Ribeiro, Tânia Pimentel, Amélia Tavares, Luís Bernando, Fernando Oliveira, Manuela Ir Vet J Research BACKGROUND: The impact of enterococci in human health has been growing for the last decades, mainly due to their resistance to several antimicrobial agents. Human consumption of contaminated meat, especially poultry, has been identified as a possible route of transmission. The aim of the present study was to evaluate and compare the antimicrobial resistance profiles and virulence genes of enterococci isolated from Portuguese conventional and free-range broiler farms. RESULTS: Antibiotic susceptibility testing showed high frequencies of resistance to tetracycline in both farming systems. Resistance to erythromycin and gentamicin were detected in about half of the isolates. Resistance to penicillin was the less frequently observed and no vancomycin resistant isolates were identified. The majority of the enterococcal isolates, from either farming systems, were resistant to more than one antibiotic, and no statistical associations were found, except for penicillin resistance which associated with the genetic clusters. No differences were found between farming systems regarding the prevalence of tet(M), erm(B), aac (6′)-Ie-aph (2″)-Ia and pbp5 genes, nevertheless pbp5 prevalence was associated with the different genetic clusters. Hemolytic activity was identified in 26.47% of all isolates and gelatinase activity in 50%. The gelE gene was identified in the majority of the isolates, whereas esp and agg genes were rarely detected. The cylA determinant was not detected in any of the isolates. CONCLUSIONS: Overall, results suggest that similar resistance patterns and virulence genes can be found in both farming systems, though enterococci in free-range conditions should be less prone to acquire further resistance genes. BioMed Central 2021-08-20 /pmc/articles/PMC8379790/ /pubmed/34416919 http://dx.doi.org/10.1186/s13620-021-00201-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Semedo-Lemsaddek, Teresa
Bettencourt Cota, João
Ribeiro, Tânia
Pimentel, Amélia
Tavares, Luís
Bernando, Fernando
Oliveira, Manuela
Resistance and virulence distribution in enterococci isolated from broilers reared in two farming systems
title Resistance and virulence distribution in enterococci isolated from broilers reared in two farming systems
title_full Resistance and virulence distribution in enterococci isolated from broilers reared in two farming systems
title_fullStr Resistance and virulence distribution in enterococci isolated from broilers reared in two farming systems
title_full_unstemmed Resistance and virulence distribution in enterococci isolated from broilers reared in two farming systems
title_short Resistance and virulence distribution in enterococci isolated from broilers reared in two farming systems
title_sort resistance and virulence distribution in enterococci isolated from broilers reared in two farming systems
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379790/
https://www.ncbi.nlm.nih.gov/pubmed/34416919
http://dx.doi.org/10.1186/s13620-021-00201-6
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