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Abnormal expression profile of plasma-derived exosomal microRNAs in patients with treatment-resistant depression

Whether microRNAs (miRNAs) from plasma exosomes might be dysregulated in patients with depression, especially treatment-resistant depression (TRD), remains unclear, based on study of which novel biomarkers and therapeutic targets could be discovered. To this end, a small sample study was performed b...

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Autores principales: Li, Lian-Di, Naveed, Muhammad, Du, Zi-Wei, Ding, Huachen, Gu, Kai, Wei, Lu-Lu, Zhou, Ya-Ping, Meng, Fan, Wang, Chun, Han, Feng, Zhou, Qi-Gang, Zhang, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379796/
https://www.ncbi.nlm.nih.gov/pubmed/34419170
http://dx.doi.org/10.1186/s40246-021-00354-z
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author Li, Lian-Di
Naveed, Muhammad
Du, Zi-Wei
Ding, Huachen
Gu, Kai
Wei, Lu-Lu
Zhou, Ya-Ping
Meng, Fan
Wang, Chun
Han, Feng
Zhou, Qi-Gang
Zhang, Jing
author_facet Li, Lian-Di
Naveed, Muhammad
Du, Zi-Wei
Ding, Huachen
Gu, Kai
Wei, Lu-Lu
Zhou, Ya-Ping
Meng, Fan
Wang, Chun
Han, Feng
Zhou, Qi-Gang
Zhang, Jing
author_sort Li, Lian-Di
collection PubMed
description Whether microRNAs (miRNAs) from plasma exosomes might be dysregulated in patients with depression, especially treatment-resistant depression (TRD), remains unclear, based on study of which novel biomarkers and therapeutic targets could be discovered. To this end, a small sample study was performed by isolation of plasma exosomes from patients with TRD diagnosed by Hamilton scale. In this study, 4 peripheral plasma samples from patients with TRD and 4 healthy controls were collected for extraction of plasma exosomes. Exosomal miRNAs were analyzed by miRNA sequencing, followed by image collection, expression difference analysis, target gene GO enrichment analysis, and KEGG pathway enrichment analysis. Compared with the healthy controls, 2 miRNAs in the plasma exosomes of patients with TRD showed significant differences in expression, among which has-miR-335-5p were significantly upregulated and has-miR-1292-3p were significantly downregulated. Go and KEGG analysis showed that dysregulated miRNAs affect postsynaptic density and axonogenesis as well as the signaling pathway of axon formation and cell growths. The identification of these miRNAs and their target genes may provide novel biomarkers for improving diagnosis accuracy and treatment effectiveness of TRD. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40246-021-00354-z.
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spelling pubmed-83797962021-08-23 Abnormal expression profile of plasma-derived exosomal microRNAs in patients with treatment-resistant depression Li, Lian-Di Naveed, Muhammad Du, Zi-Wei Ding, Huachen Gu, Kai Wei, Lu-Lu Zhou, Ya-Ping Meng, Fan Wang, Chun Han, Feng Zhou, Qi-Gang Zhang, Jing Hum Genomics Primary Research Whether microRNAs (miRNAs) from plasma exosomes might be dysregulated in patients with depression, especially treatment-resistant depression (TRD), remains unclear, based on study of which novel biomarkers and therapeutic targets could be discovered. To this end, a small sample study was performed by isolation of plasma exosomes from patients with TRD diagnosed by Hamilton scale. In this study, 4 peripheral plasma samples from patients with TRD and 4 healthy controls were collected for extraction of plasma exosomes. Exosomal miRNAs were analyzed by miRNA sequencing, followed by image collection, expression difference analysis, target gene GO enrichment analysis, and KEGG pathway enrichment analysis. Compared with the healthy controls, 2 miRNAs in the plasma exosomes of patients with TRD showed significant differences in expression, among which has-miR-335-5p were significantly upregulated and has-miR-1292-3p were significantly downregulated. Go and KEGG analysis showed that dysregulated miRNAs affect postsynaptic density and axonogenesis as well as the signaling pathway of axon formation and cell growths. The identification of these miRNAs and their target genes may provide novel biomarkers for improving diagnosis accuracy and treatment effectiveness of TRD. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40246-021-00354-z. BioMed Central 2021-08-21 /pmc/articles/PMC8379796/ /pubmed/34419170 http://dx.doi.org/10.1186/s40246-021-00354-z Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Primary Research
Li, Lian-Di
Naveed, Muhammad
Du, Zi-Wei
Ding, Huachen
Gu, Kai
Wei, Lu-Lu
Zhou, Ya-Ping
Meng, Fan
Wang, Chun
Han, Feng
Zhou, Qi-Gang
Zhang, Jing
Abnormal expression profile of plasma-derived exosomal microRNAs in patients with treatment-resistant depression
title Abnormal expression profile of plasma-derived exosomal microRNAs in patients with treatment-resistant depression
title_full Abnormal expression profile of plasma-derived exosomal microRNAs in patients with treatment-resistant depression
title_fullStr Abnormal expression profile of plasma-derived exosomal microRNAs in patients with treatment-resistant depression
title_full_unstemmed Abnormal expression profile of plasma-derived exosomal microRNAs in patients with treatment-resistant depression
title_short Abnormal expression profile of plasma-derived exosomal microRNAs in patients with treatment-resistant depression
title_sort abnormal expression profile of plasma-derived exosomal micrornas in patients with treatment-resistant depression
topic Primary Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379796/
https://www.ncbi.nlm.nih.gov/pubmed/34419170
http://dx.doi.org/10.1186/s40246-021-00354-z
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