Cargando…
Natural variation identifies SNI1, the SMC5/6 component, as a modifier of meiotic crossover in Arabidopsis
The frequency and distribution of meiotic crossovers are tightly controlled; however, variation in this process can be observed both within and between species. Using crosses of two natural Arabidopsis thaliana accessions, Col and Ler, we mapped a crossover modifier locus to semidominant polymorphis...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379953/ https://www.ncbi.nlm.nih.gov/pubmed/34385313 http://dx.doi.org/10.1073/pnas.2021970118 |
_version_ | 1783741106145460224 |
---|---|
author | Zhu, Longfei Fernández-Jiménez, Nadia Szymanska-Lejman, Maja Pelé, Alexandre Underwood, Charles J. Serra, Heïdi Lambing, Christophe Dluzewska, Julia Bieluszewski, Tomasz Pradillo, Mónica Henderson, Ian R. Ziolkowski, Piotr A. |
author_facet | Zhu, Longfei Fernández-Jiménez, Nadia Szymanska-Lejman, Maja Pelé, Alexandre Underwood, Charles J. Serra, Heïdi Lambing, Christophe Dluzewska, Julia Bieluszewski, Tomasz Pradillo, Mónica Henderson, Ian R. Ziolkowski, Piotr A. |
author_sort | Zhu, Longfei |
collection | PubMed |
description | The frequency and distribution of meiotic crossovers are tightly controlled; however, variation in this process can be observed both within and between species. Using crosses of two natural Arabidopsis thaliana accessions, Col and Ler, we mapped a crossover modifier locus to semidominant polymorphisms in SUPPRESSOR OF NPR1-1 INDUCIBLE 1 (SNI1), which encodes a component of the SMC5/6 complex. The sni1 mutant exhibits a modified pattern of recombination across the genome with crossovers elevated in chromosome distal regions but reduced in pericentromeres. Mutations in SNI1 result in reduced crossover interference and can partially restore the fertility of a Class I crossover pathway mutant, which suggests that the protein affects noninterfering crossover repair. Therefore, we tested genetic interactions between SNI1 and both RECQ4 and FANCM DNA helicases, which showed that additional Class II crossovers observed in the sni1 mutant are FANCM independent. Furthermore, genetic analysis of other SMC5/6 mutants confirms the observations of crossover redistribution made for SNI1. The study reveals the importance of the SMC5/6 complex in ensuring the proper progress of meiotic recombination in plants. |
format | Online Article Text |
id | pubmed-8379953 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-83799532021-08-30 Natural variation identifies SNI1, the SMC5/6 component, as a modifier of meiotic crossover in Arabidopsis Zhu, Longfei Fernández-Jiménez, Nadia Szymanska-Lejman, Maja Pelé, Alexandre Underwood, Charles J. Serra, Heïdi Lambing, Christophe Dluzewska, Julia Bieluszewski, Tomasz Pradillo, Mónica Henderson, Ian R. Ziolkowski, Piotr A. Proc Natl Acad Sci U S A Biological Sciences The frequency and distribution of meiotic crossovers are tightly controlled; however, variation in this process can be observed both within and between species. Using crosses of two natural Arabidopsis thaliana accessions, Col and Ler, we mapped a crossover modifier locus to semidominant polymorphisms in SUPPRESSOR OF NPR1-1 INDUCIBLE 1 (SNI1), which encodes a component of the SMC5/6 complex. The sni1 mutant exhibits a modified pattern of recombination across the genome with crossovers elevated in chromosome distal regions but reduced in pericentromeres. Mutations in SNI1 result in reduced crossover interference and can partially restore the fertility of a Class I crossover pathway mutant, which suggests that the protein affects noninterfering crossover repair. Therefore, we tested genetic interactions between SNI1 and both RECQ4 and FANCM DNA helicases, which showed that additional Class II crossovers observed in the sni1 mutant are FANCM independent. Furthermore, genetic analysis of other SMC5/6 mutants confirms the observations of crossover redistribution made for SNI1. The study reveals the importance of the SMC5/6 complex in ensuring the proper progress of meiotic recombination in plants. National Academy of Sciences 2021-08-17 2021-08-12 /pmc/articles/PMC8379953/ /pubmed/34385313 http://dx.doi.org/10.1073/pnas.2021970118 Text en Copyright © 2021 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Biological Sciences Zhu, Longfei Fernández-Jiménez, Nadia Szymanska-Lejman, Maja Pelé, Alexandre Underwood, Charles J. Serra, Heïdi Lambing, Christophe Dluzewska, Julia Bieluszewski, Tomasz Pradillo, Mónica Henderson, Ian R. Ziolkowski, Piotr A. Natural variation identifies SNI1, the SMC5/6 component, as a modifier of meiotic crossover in Arabidopsis |
title | Natural variation identifies SNI1, the SMC5/6 component, as a modifier of meiotic crossover in Arabidopsis |
title_full | Natural variation identifies SNI1, the SMC5/6 component, as a modifier of meiotic crossover in Arabidopsis |
title_fullStr | Natural variation identifies SNI1, the SMC5/6 component, as a modifier of meiotic crossover in Arabidopsis |
title_full_unstemmed | Natural variation identifies SNI1, the SMC5/6 component, as a modifier of meiotic crossover in Arabidopsis |
title_short | Natural variation identifies SNI1, the SMC5/6 component, as a modifier of meiotic crossover in Arabidopsis |
title_sort | natural variation identifies sni1, the smc5/6 component, as a modifier of meiotic crossover in arabidopsis |
topic | Biological Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379953/ https://www.ncbi.nlm.nih.gov/pubmed/34385313 http://dx.doi.org/10.1073/pnas.2021970118 |
work_keys_str_mv | AT zhulongfei naturalvariationidentifiessni1thesmc56componentasamodifierofmeioticcrossoverinarabidopsis AT fernandezjimeneznadia naturalvariationidentifiessni1thesmc56componentasamodifierofmeioticcrossoverinarabidopsis AT szymanskalejmanmaja naturalvariationidentifiessni1thesmc56componentasamodifierofmeioticcrossoverinarabidopsis AT pelealexandre naturalvariationidentifiessni1thesmc56componentasamodifierofmeioticcrossoverinarabidopsis AT underwoodcharlesj naturalvariationidentifiessni1thesmc56componentasamodifierofmeioticcrossoverinarabidopsis AT serraheidi naturalvariationidentifiessni1thesmc56componentasamodifierofmeioticcrossoverinarabidopsis AT lambingchristophe naturalvariationidentifiessni1thesmc56componentasamodifierofmeioticcrossoverinarabidopsis AT dluzewskajulia naturalvariationidentifiessni1thesmc56componentasamodifierofmeioticcrossoverinarabidopsis AT bieluszewskitomasz naturalvariationidentifiessni1thesmc56componentasamodifierofmeioticcrossoverinarabidopsis AT pradillomonica naturalvariationidentifiessni1thesmc56componentasamodifierofmeioticcrossoverinarabidopsis AT hendersonianr naturalvariationidentifiessni1thesmc56componentasamodifierofmeioticcrossoverinarabidopsis AT ziolkowskipiotra naturalvariationidentifiessni1thesmc56componentasamodifierofmeioticcrossoverinarabidopsis |