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Assessment the using of silica nanoparticles (SiO(2)NPs) biosynthesized from rice husks by Trichoderma harzianum MF780864 as water lead adsorbent for immune status of Nile tilapia (Oreochromis niloticus)

Rice husks (RHs) was used as a substrate for biosynthesis of high-value Silica nanoparticles (SiO(2)NPs). An isolate of Trichoderma harzianum MF780864 (T. harzianum) was isolated and identified based on the Internal Transcribed Spacers (ITS) sequences; it showed the potentiality to induce SiO(2)NPs...

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Autores principales: El-Gazzar, Nashwa, Almanaa, Taghreed N., Reda, Rasha M., El Gaafary, M.N., Rashwan, A.A., Mahsoub, Fatma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8381041/
https://www.ncbi.nlm.nih.gov/pubmed/34466090
http://dx.doi.org/10.1016/j.sjbs.2021.05.027
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author El-Gazzar, Nashwa
Almanaa, Taghreed N.
Reda, Rasha M.
El Gaafary, M.N.
Rashwan, A.A.
Mahsoub, Fatma
author_facet El-Gazzar, Nashwa
Almanaa, Taghreed N.
Reda, Rasha M.
El Gaafary, M.N.
Rashwan, A.A.
Mahsoub, Fatma
author_sort El-Gazzar, Nashwa
collection PubMed
description Rice husks (RHs) was used as a substrate for biosynthesis of high-value Silica nanoparticles (SiO(2)NPs). An isolate of Trichoderma harzianum MF780864 (T. harzianum) was isolated and identified based on the Internal Transcribed Spacers (ITS) sequences; it showed the potentiality to induce SiO(2)NPs in the process of RHs biotransformation. SiO(2)NPs were produced extracellularly and their size was of about 89 nm. SiO(2)NPs characterized by oval, rod and cubical particles by using Transmission Electron Microscope (TEM).The Fourier transform infrared spectroscopy (FTIR) confirmed the presence of various functional groups of biomolecules and capping protein, encapsulating SiO(2)NPs. Water and fish samples were collected from private fish farms in El-Sharkia Governorate, Egypt. Lead (Pb) was detected from water and fish samples at its highest concentration at about 0.088 mg/L. The adsorption capacity of Pb by SiO(2)NPs was evaluated by testing different concentrations of SiO(2)NPs viz. 1, 2, and 3 mg/L, wherein 1 mg/L revealed the highest Pb adsorption efficiency. Within laboratory trials, the results indicated that highest Pb adsorption efficiency revealed through the increasing of SiO(2)NPs concentrations until 120 h. In vivo trial that lasted for 8 weeks, Nile tilapia (Oreochromis niloticus) (29.78 ± 0.36 g body weight) supplemented with 0.088 mg/L Pb was divided into four experimental groups having three replicates (15 fish/replicate; 45 fish/group). The results showed that SiO(2)NPs supplementation through water revealed significant increase in growth and hematological parameters of O. niloticus. Moreover, enhancement of antioxidant capacity (TAC), and immune related gene expression of IL-1β were increased in the presence of SiO(2)NPs compared with the groups of Pb exposure. Moreover, Pb residue level in fish muscles was noticeably decreased in the SiO(2)NPs treated groups. Thus, this research opens up other possibilities in the field of using SiO(2)NPs as a lead adsorbent for water bioremediation.
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spelling pubmed-83810412021-08-30 Assessment the using of silica nanoparticles (SiO(2)NPs) biosynthesized from rice husks by Trichoderma harzianum MF780864 as water lead adsorbent for immune status of Nile tilapia (Oreochromis niloticus) El-Gazzar, Nashwa Almanaa, Taghreed N. Reda, Rasha M. El Gaafary, M.N. Rashwan, A.A. Mahsoub, Fatma Saudi J Biol Sci Original Article Rice husks (RHs) was used as a substrate for biosynthesis of high-value Silica nanoparticles (SiO(2)NPs). An isolate of Trichoderma harzianum MF780864 (T. harzianum) was isolated and identified based on the Internal Transcribed Spacers (ITS) sequences; it showed the potentiality to induce SiO(2)NPs in the process of RHs biotransformation. SiO(2)NPs were produced extracellularly and their size was of about 89 nm. SiO(2)NPs characterized by oval, rod and cubical particles by using Transmission Electron Microscope (TEM).The Fourier transform infrared spectroscopy (FTIR) confirmed the presence of various functional groups of biomolecules and capping protein, encapsulating SiO(2)NPs. Water and fish samples were collected from private fish farms in El-Sharkia Governorate, Egypt. Lead (Pb) was detected from water and fish samples at its highest concentration at about 0.088 mg/L. The adsorption capacity of Pb by SiO(2)NPs was evaluated by testing different concentrations of SiO(2)NPs viz. 1, 2, and 3 mg/L, wherein 1 mg/L revealed the highest Pb adsorption efficiency. Within laboratory trials, the results indicated that highest Pb adsorption efficiency revealed through the increasing of SiO(2)NPs concentrations until 120 h. In vivo trial that lasted for 8 weeks, Nile tilapia (Oreochromis niloticus) (29.78 ± 0.36 g body weight) supplemented with 0.088 mg/L Pb was divided into four experimental groups having three replicates (15 fish/replicate; 45 fish/group). The results showed that SiO(2)NPs supplementation through water revealed significant increase in growth and hematological parameters of O. niloticus. Moreover, enhancement of antioxidant capacity (TAC), and immune related gene expression of IL-1β were increased in the presence of SiO(2)NPs compared with the groups of Pb exposure. Moreover, Pb residue level in fish muscles was noticeably decreased in the SiO(2)NPs treated groups. Thus, this research opens up other possibilities in the field of using SiO(2)NPs as a lead adsorbent for water bioremediation. Elsevier 2021-09 2021-05-21 /pmc/articles/PMC8381041/ /pubmed/34466090 http://dx.doi.org/10.1016/j.sjbs.2021.05.027 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Original Article
El-Gazzar, Nashwa
Almanaa, Taghreed N.
Reda, Rasha M.
El Gaafary, M.N.
Rashwan, A.A.
Mahsoub, Fatma
Assessment the using of silica nanoparticles (SiO(2)NPs) biosynthesized from rice husks by Trichoderma harzianum MF780864 as water lead adsorbent for immune status of Nile tilapia (Oreochromis niloticus)
title Assessment the using of silica nanoparticles (SiO(2)NPs) biosynthesized from rice husks by Trichoderma harzianum MF780864 as water lead adsorbent for immune status of Nile tilapia (Oreochromis niloticus)
title_full Assessment the using of silica nanoparticles (SiO(2)NPs) biosynthesized from rice husks by Trichoderma harzianum MF780864 as water lead adsorbent for immune status of Nile tilapia (Oreochromis niloticus)
title_fullStr Assessment the using of silica nanoparticles (SiO(2)NPs) biosynthesized from rice husks by Trichoderma harzianum MF780864 as water lead adsorbent for immune status of Nile tilapia (Oreochromis niloticus)
title_full_unstemmed Assessment the using of silica nanoparticles (SiO(2)NPs) biosynthesized from rice husks by Trichoderma harzianum MF780864 as water lead adsorbent for immune status of Nile tilapia (Oreochromis niloticus)
title_short Assessment the using of silica nanoparticles (SiO(2)NPs) biosynthesized from rice husks by Trichoderma harzianum MF780864 as water lead adsorbent for immune status of Nile tilapia (Oreochromis niloticus)
title_sort assessment the using of silica nanoparticles (sio(2)nps) biosynthesized from rice husks by trichoderma harzianum mf780864 as water lead adsorbent for immune status of nile tilapia (oreochromis niloticus)
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8381041/
https://www.ncbi.nlm.nih.gov/pubmed/34466090
http://dx.doi.org/10.1016/j.sjbs.2021.05.027
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