Replication of cowpox virus in macrophages is dependent on the host range factor p28/N1R

Zoonotic orthopoxvirus infections continue to represent a threat to human health. The disease caused by distinct orthopoxviruses differs in terms of symptoms and severity, which may be explained by the unique repertoire of virus factors that modulate the host’s immune response and cellular machinery...

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Autores principales: Bourquain, Daniel, Schrick, Livia, Tischer, Bernd Karsten, Osterrieder, Klaus, Schaade, Lars, Nitsche, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8381512/
https://www.ncbi.nlm.nih.gov/pubmed/34425838
http://dx.doi.org/10.1186/s12985-021-01640-x
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author Bourquain, Daniel
Schrick, Livia
Tischer, Bernd Karsten
Osterrieder, Klaus
Schaade, Lars
Nitsche, Andreas
author_facet Bourquain, Daniel
Schrick, Livia
Tischer, Bernd Karsten
Osterrieder, Klaus
Schaade, Lars
Nitsche, Andreas
author_sort Bourquain, Daniel
collection PubMed
description Zoonotic orthopoxvirus infections continue to represent a threat to human health. The disease caused by distinct orthopoxviruses differs in terms of symptoms and severity, which may be explained by the unique repertoire of virus factors that modulate the host’s immune response and cellular machinery. We report here on the construction of recombinant cowpox viruses (CPXV) which either lack the host range factor p28 completely or express truncated variants of p28. We show that p28 is essential for CPXV replication in macrophages of human or mouse origin and that the C-terminal RING finger domain of p28 is necessary to allow CPXV replication in macrophages. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12985-021-01640-x.
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spelling pubmed-83815122021-08-23 Replication of cowpox virus in macrophages is dependent on the host range factor p28/N1R Bourquain, Daniel Schrick, Livia Tischer, Bernd Karsten Osterrieder, Klaus Schaade, Lars Nitsche, Andreas Virol J Short Report Zoonotic orthopoxvirus infections continue to represent a threat to human health. The disease caused by distinct orthopoxviruses differs in terms of symptoms and severity, which may be explained by the unique repertoire of virus factors that modulate the host’s immune response and cellular machinery. We report here on the construction of recombinant cowpox viruses (CPXV) which either lack the host range factor p28 completely or express truncated variants of p28. We show that p28 is essential for CPXV replication in macrophages of human or mouse origin and that the C-terminal RING finger domain of p28 is necessary to allow CPXV replication in macrophages. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12985-021-01640-x. BioMed Central 2021-08-23 /pmc/articles/PMC8381512/ /pubmed/34425838 http://dx.doi.org/10.1186/s12985-021-01640-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Short Report
Bourquain, Daniel
Schrick, Livia
Tischer, Bernd Karsten
Osterrieder, Klaus
Schaade, Lars
Nitsche, Andreas
Replication of cowpox virus in macrophages is dependent on the host range factor p28/N1R
title Replication of cowpox virus in macrophages is dependent on the host range factor p28/N1R
title_full Replication of cowpox virus in macrophages is dependent on the host range factor p28/N1R
title_fullStr Replication of cowpox virus in macrophages is dependent on the host range factor p28/N1R
title_full_unstemmed Replication of cowpox virus in macrophages is dependent on the host range factor p28/N1R
title_short Replication of cowpox virus in macrophages is dependent on the host range factor p28/N1R
title_sort replication of cowpox virus in macrophages is dependent on the host range factor p28/n1r
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8381512/
https://www.ncbi.nlm.nih.gov/pubmed/34425838
http://dx.doi.org/10.1186/s12985-021-01640-x
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