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Integrated single-cell analysis unveils diverging immune features of COVID-19, influenza, and other community-acquired pneumonia

The exact immunopathophysiology of community-acquired pneumonia (CAP) caused by SARS-CoV-2 (COVID-19) remains clouded by a general lack of relevant disease controls. The scarcity of single-cell investigations in the broader population of patients with CAP renders it difficult to distinguish immune f...

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Autores principales: Schuurman, Alex R, Reijnders, Tom DY, Saris, Anno, Ramirez Moral, Ivan, Schinkel, Michiel, de Brabander, Justin, van Linge, Christine, Vermeulen, Louis, Scicluna, Brendon P, Wiersinga, W Joost, Vieira Braga, Felipe A, van der Poll, Tom
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8382293/
https://www.ncbi.nlm.nih.gov/pubmed/34424199
http://dx.doi.org/10.7554/eLife.69661
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author Schuurman, Alex R
Reijnders, Tom DY
Saris, Anno
Ramirez Moral, Ivan
Schinkel, Michiel
de Brabander, Justin
van Linge, Christine
Vermeulen, Louis
Scicluna, Brendon P
Wiersinga, W Joost
Vieira Braga, Felipe A
van der Poll, Tom
author_facet Schuurman, Alex R
Reijnders, Tom DY
Saris, Anno
Ramirez Moral, Ivan
Schinkel, Michiel
de Brabander, Justin
van Linge, Christine
Vermeulen, Louis
Scicluna, Brendon P
Wiersinga, W Joost
Vieira Braga, Felipe A
van der Poll, Tom
author_sort Schuurman, Alex R
collection PubMed
description The exact immunopathophysiology of community-acquired pneumonia (CAP) caused by SARS-CoV-2 (COVID-19) remains clouded by a general lack of relevant disease controls. The scarcity of single-cell investigations in the broader population of patients with CAP renders it difficult to distinguish immune features unique to COVID-19 from the common characteristics of a dysregulated host response to pneumonia. We performed integrated single-cell transcriptomic and proteomic analyses in peripheral blood mononuclear cells from a matched cohort of eight patients with COVID-19, eight patients with CAP caused by Influenza A or other pathogens, and four non-infectious control subjects. Using this balanced, multi-omics approach, we describe shared and diverging transcriptional and phenotypic patterns—including increased levels of type I interferon-stimulated natural killer cells in COVID-19, cytotoxic CD8 T EMRA cells in both COVID-19 and influenza, and distinctive monocyte compositions between all groups—and thereby expand our understanding of the peripheral immune response in different etiologies of pneumonia.
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spelling pubmed-83822932021-08-25 Integrated single-cell analysis unveils diverging immune features of COVID-19, influenza, and other community-acquired pneumonia Schuurman, Alex R Reijnders, Tom DY Saris, Anno Ramirez Moral, Ivan Schinkel, Michiel de Brabander, Justin van Linge, Christine Vermeulen, Louis Scicluna, Brendon P Wiersinga, W Joost Vieira Braga, Felipe A van der Poll, Tom eLife Immunology and Inflammation The exact immunopathophysiology of community-acquired pneumonia (CAP) caused by SARS-CoV-2 (COVID-19) remains clouded by a general lack of relevant disease controls. The scarcity of single-cell investigations in the broader population of patients with CAP renders it difficult to distinguish immune features unique to COVID-19 from the common characteristics of a dysregulated host response to pneumonia. We performed integrated single-cell transcriptomic and proteomic analyses in peripheral blood mononuclear cells from a matched cohort of eight patients with COVID-19, eight patients with CAP caused by Influenza A or other pathogens, and four non-infectious control subjects. Using this balanced, multi-omics approach, we describe shared and diverging transcriptional and phenotypic patterns—including increased levels of type I interferon-stimulated natural killer cells in COVID-19, cytotoxic CD8 T EMRA cells in both COVID-19 and influenza, and distinctive monocyte compositions between all groups—and thereby expand our understanding of the peripheral immune response in different etiologies of pneumonia. eLife Sciences Publications, Ltd 2021-08-23 /pmc/articles/PMC8382293/ /pubmed/34424199 http://dx.doi.org/10.7554/eLife.69661 Text en © 2021, Schuurman et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Immunology and Inflammation
Schuurman, Alex R
Reijnders, Tom DY
Saris, Anno
Ramirez Moral, Ivan
Schinkel, Michiel
de Brabander, Justin
van Linge, Christine
Vermeulen, Louis
Scicluna, Brendon P
Wiersinga, W Joost
Vieira Braga, Felipe A
van der Poll, Tom
Integrated single-cell analysis unveils diverging immune features of COVID-19, influenza, and other community-acquired pneumonia
title Integrated single-cell analysis unveils diverging immune features of COVID-19, influenza, and other community-acquired pneumonia
title_full Integrated single-cell analysis unveils diverging immune features of COVID-19, influenza, and other community-acquired pneumonia
title_fullStr Integrated single-cell analysis unveils diverging immune features of COVID-19, influenza, and other community-acquired pneumonia
title_full_unstemmed Integrated single-cell analysis unveils diverging immune features of COVID-19, influenza, and other community-acquired pneumonia
title_short Integrated single-cell analysis unveils diverging immune features of COVID-19, influenza, and other community-acquired pneumonia
title_sort integrated single-cell analysis unveils diverging immune features of covid-19, influenza, and other community-acquired pneumonia
topic Immunology and Inflammation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8382293/
https://www.ncbi.nlm.nih.gov/pubmed/34424199
http://dx.doi.org/10.7554/eLife.69661
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