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Dental Implants Surface in vitro Decontamination Protocols
Objective The number of patients rehabilitated with dental implants has contributed to increased incidence of peri-implant diseases. Due to complex and difficult treatment, peri-implantitis is a challenge and an efficient clinical protocol is not yet established. Aim of this study was to evaluate t...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Thieme Medical and Scientific Publishers Pvt. Ltd.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8382458/ https://www.ncbi.nlm.nih.gov/pubmed/33285571 http://dx.doi.org/10.1055/s-0040-1721550 |
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author | Batalha, Vanessa Coelho Bueno, Raquel Abreu Fronchetti Junior, Edemar Mariano, José Ricardo Santin, Gabriela Cristina Freitas, Karina Maria Salvatore Ortiz, Mariana Aparecida Lopes Salmeron, Samira |
author_facet | Batalha, Vanessa Coelho Bueno, Raquel Abreu Fronchetti Junior, Edemar Mariano, José Ricardo Santin, Gabriela Cristina Freitas, Karina Maria Salvatore Ortiz, Mariana Aparecida Lopes Salmeron, Samira |
author_sort | Batalha, Vanessa Coelho |
collection | PubMed |
description | Objective The number of patients rehabilitated with dental implants has contributed to increased incidence of peri-implant diseases. Due to complex and difficult treatment, peri-implantitis is a challenge and an efficient clinical protocol is not yet established. Aim of this study was to evaluate the efficacy of two protocols for in vitro decontamination of dental implants surface. Materials and Methods Twenty titanium implants (BioHE-Bioconect) were used. Implants were divided into five groups ( n = 4). NC group (negative control): sterile implants; PC group (positive control): biofilm contaminated implants; S group: biofilm contaminated implants, brushed with sterile saline; SB group: biofilm contaminated implants, brushed with sterile saline and treated with air-powder abrasive system with sodium bicarbonate (1 minute); and antimicrobial photodynamic therapy (aPDT) group: biofilm contaminated implants, brushed with sterile saline and treated with antimicrobial photodynamic therapy (red laser + toluidine blue O). The implants were contaminated in vitro with subgingival biofilm and distributed in groups PC, S, SB, and aPDT. Each group received the respective decontamination treatment, except groups NC and PC. Then, all implants were placed in tubes containing culture medium for later sowing and counting of colony-forming units (CFUs). Statistical Analysis One-way analysis of variance and Tukey tests were performed, at 5% significance level. Results Significantly fewer CFUs were observed in the aPDT group (19.38 × 10 (5) ) when compared with groups SB (26.88 × 10 (5) ), S (47.75 × 10 (5) ), and PC (59.88 × 10 (5) ) ( p < 0.01). Both the aPDT and SB groups were statistically different from the NC group ( p < 0.01). Conclusion Proposed protocols, using air-powder abrasive system with sodium bicarbonate and aPDT, showed to be efficacious in the decontamination of dental implants surface in vitro . |
format | Online Article Text |
id | pubmed-8382458 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Thieme Medical and Scientific Publishers Pvt. Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83824582021-08-24 Dental Implants Surface in vitro Decontamination Protocols Batalha, Vanessa Coelho Bueno, Raquel Abreu Fronchetti Junior, Edemar Mariano, José Ricardo Santin, Gabriela Cristina Freitas, Karina Maria Salvatore Ortiz, Mariana Aparecida Lopes Salmeron, Samira Eur J Dent Objective The number of patients rehabilitated with dental implants has contributed to increased incidence of peri-implant diseases. Due to complex and difficult treatment, peri-implantitis is a challenge and an efficient clinical protocol is not yet established. Aim of this study was to evaluate the efficacy of two protocols for in vitro decontamination of dental implants surface. Materials and Methods Twenty titanium implants (BioHE-Bioconect) were used. Implants were divided into five groups ( n = 4). NC group (negative control): sterile implants; PC group (positive control): biofilm contaminated implants; S group: biofilm contaminated implants, brushed with sterile saline; SB group: biofilm contaminated implants, brushed with sterile saline and treated with air-powder abrasive system with sodium bicarbonate (1 minute); and antimicrobial photodynamic therapy (aPDT) group: biofilm contaminated implants, brushed with sterile saline and treated with antimicrobial photodynamic therapy (red laser + toluidine blue O). The implants were contaminated in vitro with subgingival biofilm and distributed in groups PC, S, SB, and aPDT. Each group received the respective decontamination treatment, except groups NC and PC. Then, all implants were placed in tubes containing culture medium for later sowing and counting of colony-forming units (CFUs). Statistical Analysis One-way analysis of variance and Tukey tests were performed, at 5% significance level. Results Significantly fewer CFUs were observed in the aPDT group (19.38 × 10 (5) ) when compared with groups SB (26.88 × 10 (5) ), S (47.75 × 10 (5) ), and PC (59.88 × 10 (5) ) ( p < 0.01). Both the aPDT and SB groups were statistically different from the NC group ( p < 0.01). Conclusion Proposed protocols, using air-powder abrasive system with sodium bicarbonate and aPDT, showed to be efficacious in the decontamination of dental implants surface in vitro . Thieme Medical and Scientific Publishers Pvt. Ltd. 2021-07 2020-12-07 /pmc/articles/PMC8382458/ /pubmed/33285571 http://dx.doi.org/10.1055/s-0040-1721550 Text en European Journal of Dentistry. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/). https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited. |
spellingShingle | Batalha, Vanessa Coelho Bueno, Raquel Abreu Fronchetti Junior, Edemar Mariano, José Ricardo Santin, Gabriela Cristina Freitas, Karina Maria Salvatore Ortiz, Mariana Aparecida Lopes Salmeron, Samira Dental Implants Surface in vitro Decontamination Protocols |
title |
Dental Implants Surface
in vitro
Decontamination Protocols
|
title_full |
Dental Implants Surface
in vitro
Decontamination Protocols
|
title_fullStr |
Dental Implants Surface
in vitro
Decontamination Protocols
|
title_full_unstemmed |
Dental Implants Surface
in vitro
Decontamination Protocols
|
title_short |
Dental Implants Surface
in vitro
Decontamination Protocols
|
title_sort | dental implants surface
in vitro
decontamination protocols |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8382458/ https://www.ncbi.nlm.nih.gov/pubmed/33285571 http://dx.doi.org/10.1055/s-0040-1721550 |
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