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Accumulation of geranylgeranylated chlorophylls in the pigment-protein complexes of Arabidopsis thaliana acclimated to green light: effects on the organization of light-harvesting complex II and photosystem II functions

Light quality significantly influences plant metabolism, growth and development. Recently, we have demonstrated that leaves of barley and other plant species grown under monochromatic green light (500–590 nm) accumulated a large pool of chlorophyll a (Chl a) intermediates with incomplete hydrogenati...

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Autores principales: Karlický, Václav, Kmecová Materová, Zuzana, Kurasová, Irena, Nezval, Jakub, Štroch, Michal, Garab, Győző, Špunda, Vladimír
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8382614/
https://www.ncbi.nlm.nih.gov/pubmed/33948813
http://dx.doi.org/10.1007/s11120-021-00827-1
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author Karlický, Václav
Kmecová Materová, Zuzana
Kurasová, Irena
Nezval, Jakub
Štroch, Michal
Garab, Győző
Špunda, Vladimír
author_facet Karlický, Václav
Kmecová Materová, Zuzana
Kurasová, Irena
Nezval, Jakub
Štroch, Michal
Garab, Győző
Špunda, Vladimír
author_sort Karlický, Václav
collection PubMed
description Light quality significantly influences plant metabolism, growth and development. Recently, we have demonstrated that leaves of barley and other plant species grown under monochromatic green light (500–590 nm) accumulated a large pool of chlorophyll a (Chl a) intermediates with incomplete hydrogenation of their phytyl chains. In this work, we studied accumulation of these geranylgeranylated Chls a and b in pigment-protein complexes (PPCs) of Arabidopsis plants acclimated to green light and their structural–functional consequences on the photosynthetic apparatus. We found that geranylgeranylated Chls are present in all major PPCs, although their presence was more pronounced in light-harvesting complex II (LHCII) and less prominent in supercomplexes of photosystem II (PSII). Accumulation of geranylgeranylated Chls hampered the formation of PSII and PSI super- and megacomplexes in the thylakoid membranes as well as their assembly into chiral macrodomains; it also lowered the temperature stability of the PPCs, especially that of LHCII trimers, which led to their monomerization and an anomaly in the photoprotective mechanism of non-photochemical quenching. Role of geranylgeranylated Chls in adverse effects on photosynthetic apparatus of plants acclimated to green light is discussed. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11120-021-00827-1.
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spelling pubmed-83826142021-09-09 Accumulation of geranylgeranylated chlorophylls in the pigment-protein complexes of Arabidopsis thaliana acclimated to green light: effects on the organization of light-harvesting complex II and photosystem II functions Karlický, Václav Kmecová Materová, Zuzana Kurasová, Irena Nezval, Jakub Štroch, Michal Garab, Győző Špunda, Vladimír Photosynth Res Original Article Light quality significantly influences plant metabolism, growth and development. Recently, we have demonstrated that leaves of barley and other plant species grown under monochromatic green light (500–590 nm) accumulated a large pool of chlorophyll a (Chl a) intermediates with incomplete hydrogenation of their phytyl chains. In this work, we studied accumulation of these geranylgeranylated Chls a and b in pigment-protein complexes (PPCs) of Arabidopsis plants acclimated to green light and their structural–functional consequences on the photosynthetic apparatus. We found that geranylgeranylated Chls are present in all major PPCs, although their presence was more pronounced in light-harvesting complex II (LHCII) and less prominent in supercomplexes of photosystem II (PSII). Accumulation of geranylgeranylated Chls hampered the formation of PSII and PSI super- and megacomplexes in the thylakoid membranes as well as their assembly into chiral macrodomains; it also lowered the temperature stability of the PPCs, especially that of LHCII trimers, which led to their monomerization and an anomaly in the photoprotective mechanism of non-photochemical quenching. Role of geranylgeranylated Chls in adverse effects on photosynthetic apparatus of plants acclimated to green light is discussed. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11120-021-00827-1. Springer Netherlands 2021-05-04 2021 /pmc/articles/PMC8382614/ /pubmed/33948813 http://dx.doi.org/10.1007/s11120-021-00827-1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Karlický, Václav
Kmecová Materová, Zuzana
Kurasová, Irena
Nezval, Jakub
Štroch, Michal
Garab, Győző
Špunda, Vladimír
Accumulation of geranylgeranylated chlorophylls in the pigment-protein complexes of Arabidopsis thaliana acclimated to green light: effects on the organization of light-harvesting complex II and photosystem II functions
title Accumulation of geranylgeranylated chlorophylls in the pigment-protein complexes of Arabidopsis thaliana acclimated to green light: effects on the organization of light-harvesting complex II and photosystem II functions
title_full Accumulation of geranylgeranylated chlorophylls in the pigment-protein complexes of Arabidopsis thaliana acclimated to green light: effects on the organization of light-harvesting complex II and photosystem II functions
title_fullStr Accumulation of geranylgeranylated chlorophylls in the pigment-protein complexes of Arabidopsis thaliana acclimated to green light: effects on the organization of light-harvesting complex II and photosystem II functions
title_full_unstemmed Accumulation of geranylgeranylated chlorophylls in the pigment-protein complexes of Arabidopsis thaliana acclimated to green light: effects on the organization of light-harvesting complex II and photosystem II functions
title_short Accumulation of geranylgeranylated chlorophylls in the pigment-protein complexes of Arabidopsis thaliana acclimated to green light: effects on the organization of light-harvesting complex II and photosystem II functions
title_sort accumulation of geranylgeranylated chlorophylls in the pigment-protein complexes of arabidopsis thaliana acclimated to green light: effects on the organization of light-harvesting complex ii and photosystem ii functions
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8382614/
https://www.ncbi.nlm.nih.gov/pubmed/33948813
http://dx.doi.org/10.1007/s11120-021-00827-1
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